ID L7LB78_9ACTN Unreviewed; 942 AA.
AC L7LB78;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00022419, ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPC {ECO:0000256|HAMAP-Rule:MF_00595};
DE Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_00595};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305, ECO:0000256|HAMAP-Rule:MF_00595};
GN Name=ppc {ECO:0000256|HAMAP-Rule:MF_00595,
GN ECO:0000313|EMBL:GAC57298.1};
GN ORFNames=GOHSU_18_00530 {ECO:0000313|EMBL:GAC57298.1};
OS Gordonia hirsuta DSM 44140 = NBRC 16056.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1121927 {ECO:0000313|EMBL:GAC57298.1, ECO:0000313|Proteomes:UP000053405};
RN [1] {ECO:0000313|EMBL:GAC57298.1, ECO:0000313|Proteomes:UP000053405}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 16056 {ECO:0000313|EMBL:GAC57298.1,
RC ECO:0000313|Proteomes:UP000053405};
RA Isaki-Nakamura S., Hosoyama A., Tsuchikane K., Katsumata H., Baba S.,
RA Yamazaki S., Fujita N.;
RT "Whole genome shotgun sequence of Gordonia hirsuta NBRC 16056.";
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC for the tricarboxylic acid cycle. {ECO:0000256|ARBA:ARBA00003670,
CC ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071, ECO:0000256|HAMAP-
CC Rule:MF_00595};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946,
CC ECO:0000256|HAMAP-Rule:MF_00595};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346, ECO:0000256|HAMAP-Rule:MF_00595}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAC57298.1}.
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DR EMBL; BANT01000018; GAC57298.1; -; Genomic_DNA.
DR RefSeq; WP_005939153.1; NZ_BANT01000018.1.
DR AlphaFoldDB; L7LB78; -.
DR STRING; 1121927.GOHSU_18_00530; -.
DR eggNOG; COG2352; Bacteria.
DR OrthoDB; 9768133at2; -.
DR Proteomes; UP000053405; Unassembled WGS sequence.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 1.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300, ECO:0000256|HAMAP-
KW Rule:MF_00595};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_00595};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|HAMAP-Rule:MF_00595};
KW Pyruvate {ECO:0000313|EMBL:GAC57298.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000053405}.
FT ACT_SITE 169
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 605
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00595,
FT ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 942 AA; 103054 MW; 0AA320E6FA0DBBBF CRC64;
MPPPESSAEI VARAVATPMI DRIVIDDTGQ AATEPLREDI RFLGGLLGEV IAAHSGEQTF
DLVENSRRDA FAIRYNEISR DDLAARYTDP TVGELLPVIR AFSSFALLAN LAEDLHRERR
RRIHQRAGDR PQPSSLAGAL SALRDAGLDD ATVGQALAHG AVVPVITAHP TETRRRTVFE
AQNTITELMR VRARTELIPE EIERIERQIT EQVLVLWQTA LIRLRRLTIG DEITTGLRYY
GASFFQVLPN LNRDVRRALT SAYPEAGLEK LSLIRMGSWI GGDRDGNPAV TGQVVNQATS
AAAATALRHH LNEVTTLRHE LAMSARLVSP ITDDLRALSG RSADDDDEPF RSALRTVRGR
LAATAQDLLG TDFLLAAERE WAATAEPYAG PEEFLADLDV IDTALRAVVD DTLADNRLGA
LREAVRTFGF HLSGLDLRQN SEMHEQVVGE LLAWAGVCPD YAGLDEARRV ELLTAELTTR
RPLTAPDAEL SELAAKELGV VAAAADAVRR FGPEAVPTYI ISMCQSVSDL LEAMVLLKEA
GLYGLDASGA PRCVVRVVPL LETIEDLQAG ASIITAALDL DFYRALLAGQ QNVQEVMLGY
SDSNKDGGYL AANWALYRAE LELVQVARQA GLSLRLFHGR GGTVGRGGGP SYDAILAQPP
GAVQGGLRLT EQGEVIAAKY AEPISARRNL ETLVAATLES TLLDTEGLSD PARAYQVLDE
LAGLARTAYG DLVHRTPGFV EYFTDSTPLS EIGALNIGSR PTSRKQTTAI SDLRAIPWVL
SWTQCRVMLP GWYGTGSAFE QWVGDGPERL AQLADYYRQW PFFRSVMSNM AQVLAKSDLG
LAARYAELVP DADLRERVFT MLATEHERTL RMYAAITGTH DLLADNDALK RSVYNRFPYL
EPLNLLQVEL LRRFRGGEDT PQIRRAIQLT MNGLATALRN SG
//
