ID   L7LD98_9ACTN            Unreviewed;       572 AA.
AC   L7LD98;
DT   06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT   06-MAR-2013, sequence version 1.
DT   27-MAR-2024, entry version 35.
DE   SubName: Full=Putative 3-ketosteroid delta(1)-dehydrogenase {ECO:0000313|EMBL:GAC57998.1};
GN   ORFNames=GOHSU_28_00530 {ECO:0000313|EMBL:GAC57998.1};
OS   Gordonia hirsuta DSM 44140 = NBRC 16056.
OC   Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC   Gordonia.
OX   NCBI_TaxID=1121927 {ECO:0000313|EMBL:GAC57998.1, ECO:0000313|Proteomes:UP000053405};
RN   [1] {ECO:0000313|EMBL:GAC57998.1, ECO:0000313|Proteomes:UP000053405}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 16056 {ECO:0000313|EMBL:GAC57998.1,
RC   ECO:0000313|Proteomes:UP000053405};
RA   Isaki-Nakamura S., Hosoyama A., Tsuchikane K., Katsumata H., Baba S.,
RA   Yamazaki S., Fujita N.;
RT   "Whole genome shotgun sequence of Gordonia hirsuta NBRC 16056.";
RL   Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAC57998.1}.
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DR   EMBL; BANT01000028; GAC57998.1; -; Genomic_DNA.
DR   RefSeq; WP_005941243.1; NZ_BANT01000028.1.
DR   AlphaFoldDB; L7LD98; -.
DR   STRING; 1121927.GOHSU_28_00530; -.
DR   eggNOG; COG1053; Bacteria.
DR   OrthoDB; 9813348at2; -.
DR   Proteomes; UP000053405; Unassembled WGS sequence.
DR   GO; GO:0033765; F:steroid dehydrogenase activity, acting on the CH-CH group of donors; IEA:UniProt.
DR   GO; GO:0008202; P:steroid metabolic process; IEA:UniProt.
DR   Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR   Gene3D; 3.90.700.10; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
DR   InterPro; IPR003953; FAD-binding_2.
DR   InterPro; IPR036188; FAD/NAD-bd_sf.
DR   InterPro; IPR027477; Succ_DH/fumarate_Rdtase_cat_sf.
DR   PANTHER; PTHR43400:SF7; FAD_BINDING_2 DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR43400; FUMARATE REDUCTASE; 1.
DR   Pfam; PF00890; FAD_binding_2; 1.
DR   SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
DR   SUPFAM; SSF56425; Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain; 1.
PE   4: Predicted;
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW   Reference proteome {ECO:0000313|Proteomes:UP000053405}.
FT   DOMAIN          7..536
FT                   /note="FAD-dependent oxidoreductase 2 FAD binding"
FT                   /evidence="ECO:0000259|Pfam:PF00890"
SQ   SEQUENCE   572 AA;  61034 MW;  83D3FC10AC79D84D CRC64;
     MPQETVDVVV VGAGGAGLSA ALTAASQGLR TVLIEKSPYW GGSTSRSGGG VWIPNNSVLR
     RDGVTDTPQE ARTYAHAIIG EHAPADKIDA YLDRGPEALD YLMAHAPLDL EWVRGYSDYY
     PEAPGGRAGG RSIEPRPFDA RRLGDDLNTL HPQYTKAPLN MVVLQSDYKW MNVGFRHWRG
     VAKMAKVAGR FTWSRSRNKK MIAMGAALAA ELLLGCRAAG VDLRLNTPLT GLLTDGDTVT
     GVRLEHDGQE VDLRARYGVI LASGGFENNA DMRARYQRAP IGTDWTTGAP SNTGDGILAA
     ARIGADLSLM DDAWWGPTIP LPKGPWFALS ERSVPGTFMV NQHGERFMNE SLPYVEAVHQ
     MYGGEFGQGE GPGANVPAWM IMDQRCRNRY LFAGIPARQP LPKSWLESGV VVKAVSIAEL
     AEKIGVDPAA LSATTARFNG FARSGTDEDF RRGASAYDNY YGDPTNKPNP NLGAVDKAPF
     YAVKMVPGDL GTKGGVDTDA TGRVLRADGS VIDGLYAAGN TSAPVMGHTY AGPGATIGPA
     MVFGYLAALD VLERSRSDAT DDPATDATAG VQ
//