ID L7W7B4_NONDD Unreviewed; 346 AA.
AC L7W7B4;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=Pseudouridine synthase {ECO:0000256|RuleBase:RU362028};
DE EC=5.4.99.- {ECO:0000256|RuleBase:RU362028};
GN Name=rluD {ECO:0000313|EMBL:AGC75686.1};
GN OrderedLocusNames=DDD_0559 {ECO:0000313|EMBL:AGC75686.1};
OS Nonlabens dokdonensis (strain DSM 17205 / KCTC 12402 / DSW-6) (Donghaeana
OS dokdonensis).
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Nonlabens.
OX NCBI_TaxID=592029 {ECO:0000313|EMBL:AGC75686.1, ECO:0000313|Proteomes:UP000011173};
RN [1] {ECO:0000313|EMBL:AGC75686.1, ECO:0000313|Proteomes:UP000011173}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 17205 / KCTC 12402 / DSW-6
RC {ECO:0000313|Proteomes:UP000011173};
RX PubMed=23292138; DOI=10.1093/gbe/evs134;
RA Kwon S.K., Kim B.K., Song J.Y., Kwak M.J., Lee C.H., Yoon J.H., Oh T.K.,
RA Kim J.F.;
RT "Genomic makeup of the marine flavobacterium Nonlabens (Donghaeana)
RT dokdonensis DSW-6 and identification of a novel class of rhodopsins.";
RL Genome Biol. Evol. 5:187-187(2013).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil.
CC {ECO:0000256|RuleBase:RU362028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC Evidence={ECO:0000256|RuleBase:RU362028};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC {ECO:0000256|ARBA:ARBA00010876, ECO:0000256|RuleBase:RU362028}.
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DR EMBL; CP001397; AGC75686.1; -; Genomic_DNA.
DR RefSeq; WP_015361184.1; NC_020156.1.
DR AlphaFoldDB; L7W7B4; -.
DR STRING; 592029.DDD_0559; -.
DR KEGG; ndo:DDD_0559; -.
DR PATRIC; fig|592029.3.peg.555; -.
DR eggNOG; COG0564; Bacteria.
DR HOGENOM; CLU_016902_4_3_10; -.
DR OrthoDB; 9807829at2; -.
DR Proteomes; UP000011173; Chromosome.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProt.
DR GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR CDD; cd00165; S4; 1.
DR Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR InterPro; IPR002942; S4_RNA-bd.
DR InterPro; IPR036986; S4_RNA-bd_sf.
DR NCBIfam; TIGR00005; rluA_subfam; 1.
DR PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR PANTHER; PTHR21600:SF44; PSEUDOU_SYNTH_2 DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00849; PseudoU_synth_2; 1.
DR Pfam; PF01479; S4; 1.
DR SMART; SM00363; S4; 1.
DR SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS01129; PSI_RLU; 1.
DR PROSITE; PS50889; S4; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|RuleBase:RU362028, ECO:0000313|EMBL:AGC75686.1};
KW RNA-binding {ECO:0000256|PROSITE-ProRule:PRU00182}.
FT DOMAIN 30..95
FT /note="RNA-binding S4"
FT /evidence="ECO:0000259|SMART:SM00363"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 153
FT /evidence="ECO:0000256|PIRSR:PIRSR606225-1"
SQ SEQUENCE 346 AA; 39423 MW; 5A793B5CEF27DA3E CRC64;
MNKEIDHEDL EDQGKHEHYQ FTAGSGQDPL RVDKYLMNFI ENATRSKIQQ GIKEGAVTVN
GEIVKSNYKV KPHDDIRVSF DHPPHESLLI AEDIPLDIVY EDDHLVVVNK PAGMVVHPGH
GNYSGTLINA LIHHFENLPN NSSNRPGLVH RIDKDTSGLL VVAKTEEAMA YLSAQFAAKT
SEREYIAIVW GNFTEKSGTV EGNIGRHPKN RLQNTVWLDD EAYKGKPAVT HYTVTEDLGY
VSVISCRLET GRTHQIRVHM KYIGHTLFND ERYGGDRILK GTNFTKYKQF VDNCFKVLPR
QALHARTLGF EHPHTKEWMS FTSVVPQDMV ECIEKWRVYA ENSKDM
//
