ID L8G2B3_PSED2 Unreviewed; 928 AA.
AC L8G2B3;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=GMDG_08333 {ECO:0000313|EMBL:ELR07262.1};
OS Pseudogymnoascus destructans (strain ATCC MYA-4855 / 20631-21) (Bat
OS white-nose syndrome fungus) (Geomyces destructans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus.
OX NCBI_TaxID=658429 {ECO:0000313|EMBL:ELR07262.1, ECO:0000313|Proteomes:UP000011064};
RN [1] {ECO:0000313|Proteomes:UP000011064}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4855 / 20631-21 {ECO:0000313|Proteomes:UP000011064};
RG The Broad Institute Genome Sequencing Platform;
RA Cuomo C.A., Blehert D.S., Lorch J.M., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Alvarado L., Arachchi H.M.,
RA Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C., Freedman E.,
RA Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heiman D.,
RA Howarth C., Larson L., Lui A., MacDonald P.J.P., Montmayeur A., Murphy C.,
RA Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The genome sequence of Geomyces destructans 20631-21.";
RL Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: ATP-binding RNA helicase involved in the biogenesis of 60S
CC ribosomal subunits and is required for the normal formation of 25S and
CC 5.8S rRNAs. {ECO:0000256|ARBA:ARBA00003706}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX54/DBP10
CC subfamily. {ECO:0000256|ARBA:ARBA00010379}.
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DR EMBL; GL573511; ELR07262.1; -; Genomic_DNA.
DR RefSeq; XP_012746794.1; XM_012891340.1.
DR AlphaFoldDB; L8G2B3; -.
DR STRING; 658429.L8G2B3; -.
DR VEuPathDB; FungiDB:GMDG_08333; -.
DR HOGENOM; CLU_003041_5_0_1; -.
DR InParanoid; L8G2B3; -.
DR OrthoDB; 5475716at2759; -.
DR Proteomes; UP000011064; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR CDD; cd17959; DEADc_DDX54; 1.
DR CDD; cd18787; SF2_C_DEAD; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR012541; DBP10_C.
DR InterPro; IPR033517; DDX54/DBP10_DEAD-box_helicase.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF8; RNA HELICASE; 1.
DR Pfam; PF08147; DBP10CT; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM01123; DBP10CT; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:ELR07262.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000011064};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 90..118
FT /note="DEAD-box RNA helicase Q"
FT /evidence="ECO:0000259|PROSITE:PS51195"
FT DOMAIN 121..293
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 338..504
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 336..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 830..928
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 90..118
FT /note="Q motif"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00552"
FT COMPBIAS 344..363
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 851..918
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 928 AA; 102117 MW; BDA3EC6578BEF639 CRC64;
MVPRNNRRAA SPAMSENEFD ITKSLFNEDV ESDDDNFGVA GEKTDIPMDG VLDMALESDG
EGDEAFIASE LAASNRKSSN VKGKSVKKGG GFQAMGLNSH LLKAIGRKGF SVPTPIQRKA
IPLILDSQDV VGMARTGSGK TAAFVIPMIE KLKAHSAKVG ARALILSPSR ELALQTLQVV
KQFGRGTDLK CVLLVGGDSL EEQFGFMASN PDIVIATPGR FLHLKVEMGL DLSSMKYVVF
DEADRLFEMG FAAQLTEILY ALPSSRQTLL FSATLPKSLV EFARAGLEEP SLVRLDAESK
ISPDLQSAFF TIKSEEKEGA LLHILHDLIR MPTGPPPAAV AAAEASKKRK RGDDGRGPKH
KPTEHSTIVF AATKHHVDYL ASLLRLSGFA VSHAYGSLDQ TARKMQVEDF RTGMTNILVV
TDVAARGIDI PVLANVVNYD FPPQPKIFVH RVGRTARAGQ RGWSYSLVKE TDAPYLLDLQ
LFLGRRLLLG RESGDSPNYA EDVIVGSVVR DKLETNQEWI NKLLRDDDDL AALRGVSVKG
ERLYLKTRNS ASSESAKRAK QSVASAQWIQ LHPLFNTETN SNEQARIDML ARIAGFRPTE
TVFEVGYKGK AGHGEAAEVM RARRQKIIPR RQKAIDDAKA EATELTDDDD TFQGISMAAP
AEDDHDDSDM EVTISGGNDV DMAEASEDEL EVTFSKAAQK GKGRTLSYKD SENFMSYTPK
NLNAAEERGY GVHSGSYNTA SQNSNFVEAA RGVTMDLTND DGAKSFAEPS QAKGMRWDKK
NSKYVARAND EDGSKGAKMI RGESGQKIAA SFQSGRFDRW RKAHKVERLP RTGESERAGG
VGATVGRGMG TRYKHKQERA PKEADKYRDD YHVRRRRVDE AKEKRVGRFR DGGGKGEIKS
TEDIRKQRQL EQRKKEKNAR PSGKNKKR
//
