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Database: UniProt
Entry: L9PIH0_9BURK
LinkDB: L9PIH0_9BURK
Original site: L9PIH0_9BURK 
ID   L9PIH0_9BURK            Unreviewed;       589 AA.
AC   L9PIH0;
DT   03-APR-2013, integrated into UniProtKB/TrEMBL.
DT   03-APR-2013, sequence version 1.
DT   10-APR-2019, entry version 45.
DE   RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993443};
DE            EC=2.7.7.- {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993444};
GN   Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN   ECO:0000313|EMBL:ELX10658.1};
GN   ORFNames=Jab_2c27570 {ECO:0000313|EMBL:ELX10658.1};
OS   Janthinobacterium sp. HH01.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Oxalobacteraceae; Janthinobacterium.
OX   NCBI_TaxID=1198452 {ECO:0000313|EMBL:ELX10658.1, ECO:0000313|Proteomes:UP000011616};
RN   [1] {ECO:0000313|EMBL:ELX10658.1, ECO:0000313|Proteomes:UP000011616}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HH01 {ECO:0000313|EMBL:ELX10658.1,
RC   ECO:0000313|Proteomes:UP000011616};
RX   PubMed=23405110; DOI=10.1371/journal.pone.0055045;
RA   Hornung C., Poehlein A., Haack F.S., Schmidt M., Dierking K.,
RA   Pohlen A., Schulenburg H., Blokesch M., Plener L., Jung K., Bonge A.,
RA   Krohn-Molt I., Utpatel C., Timmermann G., Spieck E.,
RA   Pommerening-Roser A., Bode E., Bode H.B., Daniel R., Schmeisser C.,
RA   Streit W.R.;
RT   "The Janthinobacterium sp. HH01 Genome Encodes a Homologue of the V.
RT   cholerae CqsA and L. pneumophila LqsA Autoinducer Synthases.";
RL   PLoS ONE 8:E55045-E55045(2013).
CC   -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC       molecules used as primers for DNA polymerase during DNA
CC       replication. {ECO:0000256|HAMAP-Rule:MF_00974,
CC       ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709340}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|SAAS:SAAS00709317};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC         ECO:0000256|PIRSR:PIRSR002811-1};
CC       Note=Binds 1 zinc ion per monomer. {ECO:0000256|HAMAP-
CC       Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|PIRSR:PIRSR002811-1};
CC   -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC       Rule:MF_00974}.
CC   -!- DOMAIN: Contains an N-terminal zinc-binding domain, a central core
CC       domain that contains the primase activity, and a C-terminal DnaB-
CC       binding domain. {ECO:0000256|HAMAP-Rule:MF_00974}.
CC   -!- SIMILARITY: Belongs to the DnaG primase family.
CC       {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
CC       ECO:0000256|SAAS:SAAS00709351}.
CC   -!- CAUTION: The sequence shown here is derived from an
CC       EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is
CC       preliminary data. {ECO:0000313|EMBL:ELX10658.1}.
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DR   EMBL; AMWD01000002; ELX10658.1; -; Genomic_DNA.
DR   STRING; 1198452.Jab_2c27570; -.
DR   EnsemblBacteria; ELX10658; ELX10658; Jab_2c27570.
DR   PATRIC; fig|1198452.4.peg.5323; -.
DR   Proteomes; UP000011616; Unassembled WGS sequence.
DR   GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR   Gene3D; 3.90.580.10; -; 1.
DR   Gene3D; 3.90.980.10; -; 1.
DR   HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR   InterPro; IPR013264; DNA_primase_core_N.
DR   InterPro; IPR037068; DNA_primase_core_N_sf.
DR   InterPro; IPR019475; DNA_primase_DnaB-bd.
DR   InterPro; IPR006295; DNA_primase_DnaG.
DR   InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR   InterPro; IPR030846; DnaG_bac.
DR   InterPro; IPR034151; TOPRIM_DnaG_bac.
DR   InterPro; IPR006171; TOPRIM_domain.
DR   InterPro; IPR002694; Znf_CHC2.
DR   Pfam; PF10410; DnaB_bind; 1.
DR   Pfam; PF13662; Toprim_4; 1.
DR   Pfam; PF08275; Toprim_N; 1.
DR   Pfam; PF01807; zf-CHC2; 1.
DR   PIRSF; PIRSF002811; DnaG; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SMART; SM00400; ZnF_CHCC; 1.
DR   TIGRFAMs; TIGR01391; dnaG; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Complete proteome {ECO:0000313|Proteomes:UP000011616};
KW   DNA replication {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993445};
KW   DNA-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|SAAS:SAAS00709369};
KW   DNA-directed RNA polymerase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709327};
KW   Magnesium {ECO:0000256|SAAS:SAAS00709345};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|PIRSR:PIRSR002811-1,
KW   ECO:0000256|SAAS:SAAS00709338};
KW   Nucleotidyltransferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709339,
KW   ECO:0000313|EMBL:ELX10658.1};
KW   Primosome {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709304};
KW   Reference proteome {ECO:0000313|Proteomes:UP000011616};
KW   Transcription {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00709341};
KW   Transferase {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRNR:PIRNR002811, ECO:0000256|SAAS:SAAS00993442,
KW   ECO:0000313|EMBL:ELX10658.1};
KW   Zinc {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811,
KW   ECO:0000256|PIRSR:PIRSR002811-1, ECO:0000256|SAAS:SAAS00709300};
KW   Zinc-finger {ECO:0000256|HAMAP-Rule:MF_00974,
KW   ECO:0000256|PIRSR:PIRSR002811-1, ECO:0000256|SAAS:SAAS00709301}.
FT   DOMAIN      251    333       Toprim. {ECO:0000259|PROSITE:PS50880}.
FT   ZN_FING      28     52       CHC2-type. {ECO:0000256|HAMAP-Rule:
FT                                MF_00974, ECO:0000256|PIRSR:PIRSR002811-
FT                                1}.
FT   COILED      568    588       {ECO:0000256|SAM:Coils}.
SQ   SEQUENCE   589 AA;  64719 MW;  4CACCC33A1EBCA95 CRC64;
     MLNRVDIVDV VGRYVQLKKG GANFMGLCPF HSEKSPSFTV SPTKQFYHCF GCGAHGTSIG
     FLIEYSGMGF VDAVKDLAQN VGMVVPEQDD KIPPAQRAQM AAQSLALTEA MTRASEFYRS
     QLRGATDAIA YLKNRGLTGE IAARFGLGYA PPGWDNLRSI FPDYEAVALA EAGLVIDKVD
     EDGGNRKRYD RFRERVMFPI KNTKGQVIAF GGRVLDHGEP KYLNSPETPL FSKGFELYGL
     FEARQAIRDA GYVLVTEGYM DVVALAQMGF PQAVATLGTA CTTHHVQKLL RQTDTVIFSF
     DGDKAGRRAA RRALEACLPH VTDNKTIKFL FLPTEHDPDS YVRTYGNEAF EQEIHEAMPL
     SQFLVKEAVG EHDLQSPEGR ARAQFDAKPM LQAMTPTSLR LQIVRGLAQL TQSTPSEIEV
     LFELAKPVAV ARQAPPRTGR PVPVGLELQI VRMLVAHPPL ALHIDEAAMA AFNYFGAAPA
     ERLVQLVASA QSMGPNGGFA AFAQHLKSQG DEYDALIGEI AKEPEADIDA VRMELRGIIR
     QVKTEALKQE LSQLFAAGLS SDEIGVRYRE LTAQQNQLLR EAEAELANR
//
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