ID   L9VN17_9EURY            Unreviewed;       908 AA.
AC   L9VN17;
DT   03-APR-2013, integrated into UniProtKB/TrEMBL.
DT   03-APR-2013, sequence version 1.
DT   27-MAR-2024, entry version 45.
DE   RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE            EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN   ORFNames=C496_17552 {ECO:0000313|EMBL:ELY38451.1};
OS   Natronorubrum tibetense GA33.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Natrialbales;
OC   Natrialbaceae; Natronorubrum.
OX   NCBI_TaxID=1114856 {ECO:0000313|EMBL:ELY38451.1, ECO:0000313|Proteomes:UP000011599};
RN   [1] {ECO:0000313|EMBL:ELY38451.1, ECO:0000313|Proteomes:UP000011599}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GA33 {ECO:0000313|EMBL:ELY38451.1,
RC   ECO:0000313|Proteomes:UP000011599};
RX   PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA   Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA   Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT   "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT   strategies for static and dynamic osmo-response.";
RL   PLoS Genet. 10:E1004784-E1004784(2014).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC         ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC         Evidence={ECO:0000256|ARBA:ARBA00023501};
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000256|ARBA:ARBA00001966};
CC   -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC       {ECO:0000256|ARBA:ARBA00007185}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ELY38451.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AOHW01000042; ELY38451.1; -; Genomic_DNA.
DR   RefSeq; WP_006091562.1; NZ_KB913017.1.
DR   AlphaFoldDB; L9VN17; -.
DR   STRING; 1114856.GCA_000383975_00185; -.
DR   PATRIC; fig|1114856.3.peg.3638; -.
DR   eggNOG; arCOG01697; Archaea.
DR   OrthoDB; 255at2157; -.
DR   Proteomes; UP000011599; Unassembled WGS sequence.
DR   GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR   GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd01580; AcnA_IRP_Swivel; 1.
DR   Gene3D; 6.10.190.10; -; 1.
DR   Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR   Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR   InterPro; IPR044137; AcnA_IRP_Swivel.
DR   InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR   InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR   InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR   InterPro; IPR006249; Aconitase/IRP2.
DR   InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR   InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR   InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR   NCBIfam; TIGR01341; aconitase_1; 1.
DR   PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR   PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR   Pfam; PF00330; Aconitase; 1.
DR   Pfam; PF00694; Aconitase_C; 1.
DR   PRINTS; PR00415; ACONITASE.
DR   SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR   SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR   PROSITE; PS00450; ACONITASE_1; 1.
DR   PROSITE; PS01244; ACONITASE_2; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|ARBA:ARBA00023004};
KW   Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW   Lyase {ECO:0000313|EMBL:ELY38451.1};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT   DOMAIN          77..576
FT                   /note="Aconitase/3-isopropylmalate dehydratase large
FT                   subunit alpha/beta/alpha"
FT                   /evidence="ECO:0000259|Pfam:PF00330"
FT   DOMAIN          707..833
FT                   /note="Aconitase A/isopropylmalate dehydratase small
FT                   subunit swivel"
FT                   /evidence="ECO:0000259|Pfam:PF00694"
SQ   SEQUENCE   908 AA;  99425 MW;  163DAC8A6CA978F7 CRC64;
     MAIDEFSDAI REFEHDGETY KMADLTVLEE QGLCDLEKLP VSIRVLLEAV LRNADGDMIS
     AQDVENAASW QPEVPDVEVP FQPSRVVLQD LTGVPAVVDL AALRSAADRA DKDPSIVEPE
     VPCDLVIDHS VQVDYFGSED AYEQNVELEY ERNEERYRAI KWAEQAFEDF NVVPPGTGIV
     HQVNLEHLGE VVHAREEDGE QWLLPDTLVG TDSHTPMING IGVVGWGVGG IEAEAALLGQ
     PITMTLPEVV GVELTGELPE GATATDLVLH ITEKLRQVGV VDKFVEFYGD GVSQLSVADR
     ATISNMSPEH GCTISMFPVD EKTLDYLELT GRDEDHIELV REYLEAQGLF GDHDPEFTET
     VEFDLGSVEP SLAGHKKPHA RIPMGDLDEH FPTLLEEQGV IPSGAAESDG GLVAKEQPGL
     DEKIPVTLED GTEVEIGHGS VLVSAITSCT NTSNPSVMVA AGLLARNALE QGLDVPDYVK
     TSLAPGSKVV TEYLKQADLL EDLEGLGYHV VGYGCTTCIG NAGPLAEPIE AAIDEHDLWT
     TSVLSGNRNF EARIHPKIRA NYLASPPLVV AYGLAGKMDI DLETEPIGTN DDGEEIFLED
     IWPDPEEVRQ TIHESVSAEL FKDKYASVFE GDDRWEALDA PTGDVYEWDS ESTYIREPPF
     FQDFPLEEPG VEDIADARCL LTLGDTVTTD HISPAGPFSE DLPAGQWLSE RGVEPYEFNT
     YGSRRGNHEV MMRGTFANVR IENQLLDGKE GGYTIHHPTE EETTVFEASE RYRDEDTPLI
     VMAGEELGTG SSRDWAAKGT DLLGIRATIG QSYERIYRDN LLGMGVLPLQ FEDGEGWEEL
     GLDGSEYFDI KGLDDGLEPN AELTVVAEDD DGETTEFDVT AQVGTPAAVK YVENGGVLHL
     VLRRMLTE
//