ID LEG1B_DANRE Reviewed; 364 AA.
AC Q4QRF7; Q6P0T9;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-MAR-2015, sequence version 2.
DT 27-MAR-2024, entry version 74.
DE RecName: Full=Protein leg1b {ECO:0000303|PubMed:21857963};
DE Short=Leg1-B {ECO:0000303|PubMed:21857963};
DE AltName: Full=Liver-enriched gene protein 1-B {ECO:0000303|PubMed:21857963};
DE Flags: Precursor;
GN Name=leg1b {ECO:0000303|PubMed:21857963}; ORFNames=zgc:77778;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=AB; TISSUE=Liver;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=21857963; DOI=10.1371/journal.pone.0022910;
RA Chang C., Hu M., Zhu Z., Lo L.J., Chen J., Peng J.;
RT "liver-enriched gene 1a and 1b encode novel secretory proteins essential
RT for normal liver development in zebrafish.";
RL PLoS ONE 6:E22910-E22910(2011).
CC -!- FUNCTION: Involved in early development of liver, exocrine pancreas and
CC intestine, probably through cell cycle regulation. In liver, its
CC function is partially redundant with leg1a function.
CC {ECO:0000269|PubMed:21857963}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:21857963}.
CC -!- TISSUE SPECIFICITY: Detected in all tissues tested, with the highest
CC levels in serum (at protein level). At mRNA level, only expressed in
CC liver. {ECO:0000269|PubMed:21857963}.
CC -!- DEVELOPMENTAL STAGE: Expressed as early as 24 hours post fecondation
CC (hpf), expressed at lower levels than leg1a.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown of the protein causes a
CC small liver phenotype. Morpholino which can simultaneously block the
CC translation of leg1a and leg1b, causes a more severe small liver
CC phenotype with hypoplastic exocrine pancreas and intestinal tube as
CC well. {ECO:0000269|PubMed:21857963}.
CC -!- SIMILARITY: Belongs to the LEG1 family. {ECO:0000305}.
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DR EMBL; BC065450; AAH65450.1; -; mRNA.
DR EMBL; BC097023; AAH97023.1; -; mRNA.
DR RefSeq; NP_998368.1; NM_213203.1.
DR AlphaFoldDB; Q4QRF7; -.
DR SMR; Q4QRF7; -.
DR STRING; 7955.ENSDARP00000016639; -.
DR GlyCosmos; Q4QRF7; 1 site, No reported glycans.
DR PaxDb; 7955-ENSDARP00000016639; -.
DR PeptideAtlas; Q4QRF7; -.
DR GeneID; 406484; -.
DR KEGG; dre:406484; -.
DR AGR; ZFIN:ZDB-GENE-040426-2281; -.
DR CTD; 406484; -.
DR ZFIN; ZDB-GENE-040426-2281; leg1.2.
DR eggNOG; ENOG502R60Y; Eukaryota.
DR InParanoid; Q4QRF7; -.
DR OrthoDB; 2903711at2759; -.
DR PRO; PR:Q4QRF7; -.
DR Proteomes; UP000000437; Chromosome 20.
DR GO; GO:0005615; C:extracellular space; IDA:ZFIN.
DR GO; GO:0055123; P:digestive system development; IMP:ZFIN.
DR GO; GO:0001889; P:liver development; IMP:ZFIN.
DR InterPro; IPR008499; Leg1.
DR PANTHER; PTHR18820; LEG1; 1.
DR PANTHER; PTHR18820:SF1; PROTEIN LEG1 HOMOLOG; 1.
DR Pfam; PF05612; Leg1; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..364
FT /note="Protein leg1b"
FT /id="PRO_0000252387"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 198
FT /note="Q -> H (in Ref. 1; AAH65450)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="E -> D (in Ref. 1; AAH65450)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="S -> A (in Ref. 1; AAH65450)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 364 AA; 39839 MW; 91A2E4833435314B CRC64;
MSEMGFLRSV AAVLLLAVFS HAAVVTENGL PIQWRKAPSD LSHLPISNTG VQVNPWVYSQ
RMTMLKMVIN ATNAYMSSMG PGEQENPLWS LPLQLGWKLK SGRLADPTPD SSSTCGSEAS
DPVCISPLSF FGCVNYYLSV LPFLAAVETG VVSSGGHQML IQVPAEVAQD YCSSYSDCST
KHPNTMAKWH LFFQSLRQVS QSKESDFNKK DSILGLMWAA EEESIQTASG ACTERQKLYS
SPEVSFQQSS VNLGAFISAA HYHASIERSG KFMSHLPSRV LQEADSPPNI ADLSTEENHA
LYMLSWMNSI NQLLGGSLVD LWRKAMCSAQ AREKGQAFLD DLILDPKFPG SSLWSIICVM
STSC
//
