ID LIMF_DICDI Reviewed; 197 AA.
AC Q86I44; Q553Z0;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 97.
DE RecName: Full=LIM domain-containing protein F;
GN Name=limF; ORFNames=DDB_G0275157;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, AND INTERACTION WITH CHLIM AND RAB21.
RX PubMed=15962002; DOI=10.1038/sj.emboj.7600716;
RA Khurana T., Brzostowski J.A., Kimmel A.R.;
RT "A Rab21/LIM-only/CH-LIM complex regulates phagocytosis via both activating
RT and inhibitory mechanisms.";
RL EMBO J. 24:2254-2264(2005).
CC -!- FUNCTION: Involved in the regulation of phagocytosis. May repress ChLim
CC and activate rab21. {ECO:0000269|PubMed:15962002}.
CC -!- SUBUNIT: Interacts with ChLim and rab21. {ECO:0000269|PubMed:15962002}.
CC -!- INTERACTION:
CC Q86I44; Q55GV9: ChLim; NbExp=3; IntAct=EBI-1808928, EBI-1808948;
CC Q86I44; P34142: rab21; NbExp=4; IntAct=EBI-1808928, EBI-1808935;
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DR EMBL; AAFI02000013; EAL69859.1; -; Genomic_DNA.
DR RefSeq; XP_643821.1; XM_638729.1.
DR AlphaFoldDB; Q86I44; -.
DR SMR; Q86I44; -.
DR IntAct; Q86I44; 2.
DR STRING; 44689.Q86I44; -.
DR PaxDb; 44689-DDB0231985; -.
DR EnsemblProtists; EAL69859; EAL69859; DDB_G0275157.
DR GeneID; 8619868; -.
DR KEGG; ddi:DDB_G0275157; -.
DR dictyBase; DDB_G0275157; limF.
DR eggNOG; KOG1044; Eukaryota.
DR HOGENOM; CLU_1386427_0_0_1; -.
DR InParanoid; Q86I44; -.
DR OMA; CEECKMS; -.
DR PhylomeDB; Q86I44; -.
DR PRO; PR:Q86I44; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0001891; C:phagocytic cup; IDA:dictyBase.
DR GO; GO:0008270; F:zinc ion binding; TAS:dictyBase.
DR GO; GO:0030866; P:cortical actin cytoskeleton organization; IMP:dictyBase.
DR GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR GO; GO:0050766; P:positive regulation of phagocytosis; IMP:dictyBase.
DR GO; GO:1902351; P:response to imidacloprid; IEP:dictyBase.
DR Gene3D; 2.10.110.10; Cysteine Rich Protein; 3.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24212:SF8; LIM DOMAIN CONTAINING PROTEIN; 1.
DR PANTHER; PTHR24212; ZYXIN/TRIP6; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00132; LIM; 3.
DR SUPFAM; SSF57716; Glucocorticoid receptor-like (DNA-binding domain); 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 3.
DR PROSITE; PS50023; LIM_DOMAIN_2; 3.
PE 1: Evidence at protein level;
KW LIM domain; Metal-binding; Phagocytosis; Reference proteome; Repeat; Zinc.
FT CHAIN 1..197
FT /note="LIM domain-containing protein F"
FT /id="PRO_0000328169"
FT DOMAIN 7..68
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 77..139
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 141..197
FT /note="LIM zinc-binding 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
SQ SEQUENCE 197 AA; 22734 MW; B5E7A4ECDD4DA695 CRC64;
MSGQNILKCK KCQLEITNKI ITNHNSDTFH EQCFVCKLCS TPISDPYFTD KETGDFYCAK
CEVIRNDQSK PLRESLGFCS LCYKYFRQNE DILTIDLERY HIGCLKCTIC KKGINNEKYY
REKMTSKLSN YCCEDCFEKV DKCNGCNSMT LGQTLLAMGK NYHANCFKCF KCSEIIKPNS
PYSINKQTNT PSCQKCN
//
