ID M0M7P6_9EURY Unreviewed; 741 AA.
AC M0M7P6;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=ATP-dependent DNA helicase Hel308 {ECO:0000256|HAMAP-Rule:MF_00442};
DE EC=5.6.2.4 {ECO:0000256|HAMAP-Rule:MF_00442};
GN Name=hel308 {ECO:0000256|HAMAP-Rule:MF_00442};
GN ORFNames=C447_03886 {ECO:0000313|EMBL:EMA40629.1};
OS Halococcus hamelinensis 100A6.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Halococcaceae; Halococcus.
OX NCBI_TaxID=1132509 {ECO:0000313|EMBL:EMA40629.1, ECO:0000313|Proteomes:UP000011566};
RN [1] {ECO:0000313|EMBL:EMA40629.1, ECO:0000313|Proteomes:UP000011566}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=100A6 {ECO:0000313|EMBL:EMA40629.1,
RC ECO:0000313|Proteomes:UP000011566};
RX PubMed=25393412; DOI=10.1371/journal.pgen.1004784;
RA Becker E.A., Seitzer P.M., Tritt A., Larsen D., Krusor M., Yao A.I., Wu D.,
RA Madern D., Eisen J.A., Darling A.E., Facciotti M.T.;
RT "Phylogenetically driven sequencing of extremely halophilic archaea reveals
RT strategies for static and dynamic osmo-response.";
RL PLoS Genet. 10:E1004784-E1004784(2014).
CC -!- FUNCTION: DNA-dependent ATPase and 3'-5' DNA helicase that may be
CC involved in repair of stalled replication forks. {ECO:0000256|HAMAP-
CC Rule:MF_00442}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618, ECO:0000256|HAMAP-
CC Rule:MF_00442};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617, ECO:0000256|HAMAP-
CC Rule:MF_00442};
CC -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_00442}.
CC -!- SIMILARITY: Belongs to the helicase family. Hel308 subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMA40629.1}.
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DR EMBL; AOMB01000010; EMA40629.1; -; Genomic_DNA.
DR RefSeq; WP_007691118.1; NZ_AOMB01000010.1.
DR AlphaFoldDB; M0M7P6; -.
DR PATRIC; fig|1132509.6.peg.904; -.
DR eggNOG; arCOG00553; Archaea.
DR OrthoDB; 371946at2157; -.
DR Proteomes; UP000011566; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR CDD; cd18028; DEXHc_archSki2; 1.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR Gene3D; 1.10.3380.30; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR HAMAP; MF_00442; Helicase_Hel308; 1.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR048772; Hel308-like_dom4.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR022965; Helicase_Hel308.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR47961:SF10; ATP-DEPENDENT DNA HELICASE HEL308; 1.
DR PANTHER; PTHR47961; DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21280; Helicase_dom4_arc; 1.
DR Pfam; PF14520; HHH_5; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF158702; Sec63 N-terminal domain-like; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00442};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00442};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00442}; DNA-binding {ECO:0000256|HAMAP-Rule:MF_00442};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|HAMAP-Rule:MF_00442};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00442};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_00442};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00442}; Reference proteome {ECO:0000313|Proteomes:UP000011566}.
FT DOMAIN 34..200
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 230..435
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT BINDING 29
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00442"
SQ SEQUENCE 741 AA; 80801 MW; F50358F4CAFFC49C CRC64;
MNVTELSGVP DWLPDHLRDE GVEELYPPQA EAVEAGATEG ESLVASVPTA SGKTLIAELA
MTASVARGGT ALYIVPLRAL AGEKRTEFEA FEQYGLTVGV STGNYDSDGE WLATCDIIVA
TSEKVDSLVR NGAPWIDDLS CVVADEVHLV DDGNRGPTLE VTLAKLRQRN PGLQVVALSA
TIGNPEILAE WLDAELVDST WRPIDLKKGV HYGQAVHLED GNQRELQVRD GEKATEAIVR
DTLNDGGSTL VFVNSRRNAE AAARRLAGTV ENELTNEERT QLASVAAEIR DVSDTETSED
LASAVERGGA FHHAGLASEH RELVEGAFRD RLLKVVSATP TLAAGVNTPS RRVVVRDWRR
YSGEAGGMAP LSVLEVHQMC GRAGRPGLDP YGEALLLAKS HDELDELFDR YIWTDPEAVR
SKLAAEPALR THLLATVASG FASSREGLLE FLDQTLYATQ TTEGGRLERV TDDMLDYLEV
NDFLERDGDD LTATSLGHTV SRLYLDPMSA AEIVDGLQTA EDRPSALGLF HLVSRTPDMY
ELYLRSGDRE EYTMLANERE TELLGMKPSE FEARWEDWLS ALKTARLLED WASELDESEI
TDRYSVGPGD LRGKVDTAEW LLSAAERLAG ELGLEWAPAV REARTRVANG IRTELIDLAG
VRGVGRKRAR RLYEAGIETR TDLRNADKAV VLGALRGRRK TAENVLDNVG RTDPSMADVE
ADGSVAVVGE GENDQSNLGE F
//
