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Database: UniProt
Entry: M0XRW3_HORVV
LinkDB: M0XRW3_HORVV
Original site: M0XRW3_HORVV 
ID   M0XRW3_HORVV            Unreviewed;       841 AA.
AC   M0XRW3;
DT   03-APR-2013, integrated into UniProtKB/TrEMBL.
DT   03-APR-2013, sequence version 1.
DT   31-JUL-2019, entry version 48.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
OS   Hordeum vulgare subsp. vulgare (Domesticated barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=112509 {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.8, ECO:0000313|Proteomes:UP000011116};
RN   [1] {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.8, ECO:0000313|Proteomes:UP000011116}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Morex {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.8}, and
RC   subsp. vulgare {ECO:0000313|Proteomes:UP000011116};
RX   PubMed=23075845; DOI=10.1038/nature11543;
RG   The International Barley Genome Sequencing Consortium;
RT   "A physical, genetic and functional sequence assembly of the barley
RT   genome.";
RL   Nature 491:711-716(2012).
RN   [2] {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.8}
RP   IDENTIFICATION.
RC   STRAIN=subsp. vulgare {ECO:0000313|EnsemblPlants:HORVU5Hr1G114090.8};
RG   EnsemblPlants;
RL   Submitted (OCT-2017) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
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DR   STRING; 4513.MLOC_62464.1; -.
DR   PRIDE; M0XRW3; -.
DR   EnsemblPlants; HORVU5Hr1G114090.16; HORVU5Hr1G114090.16; HORVU5Hr1G114090.
DR   EnsemblPlants; HORVU5Hr1G114090.3; HORVU5Hr1G114090.3; HORVU5Hr1G114090.
DR   EnsemblPlants; HORVU5Hr1G114090.8; HORVU5Hr1G114090.8; HORVU5Hr1G114090.
DR   Gramene; HORVU5Hr1G114090.16; HORVU5Hr1G114090.16; HORVU5Hr1G114090.
DR   Gramene; HORVU5Hr1G114090.3; HORVU5Hr1G114090.3; HORVU5Hr1G114090.
DR   Gramene; HORVU5Hr1G114090.8; HORVU5Hr1G114090.8; HORVU5Hr1G114090.
DR   OMA; GFDSHIH; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000011116; Unassembled WGS sequence.
DR   ExpressionAtlas; M0XRW3; baseline and differential.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000011116};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000011116}.
FT   DOMAIN      398    841       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    589    589       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       403    403       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       405    405       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       486    486       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       486    486       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       515    515       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       541    541       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       629    629       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     488    488       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     486    486       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   841 AA;  90156 MW;  50A73FED8936F4FB CRC64;
     MKLVPREAEK LALHGAGFLA QKRLARGLRL NYTEAIALIA SQILEFVRDG DKTVTDLMDL
     GKQMLGRRQV LPAVPYLLDT VQVEGTFMDG TKLITVHDPI SSDDGNLELA LHGSYLPVPS
     LEKFSGSDVE DYPGEVHFCS GRIILNLHRR ALTLKVVNKA DRPIQIGSHY HFIEANPYLV
     FDRLRAYGMR LNIPAGTAVR FEPGDAKRVT LVSIGGRKVI RGGNGIADGA VDSSQLNEVI
     QKITENCFGH EDYPDASEGL IGDGIFDCSV DHEKYSSMYG PTTGDKIRLG DTDLFAEIEK
     DFAVYGDECI FGGGKVLRDG MGQSAGYPAS ASLDTVITNA VVIDYTGIYK ADIGIKDGLI
     IAIGKAGNPD VMDGVHSNMI VGVNTEVIAA QGMIVTAGGI DCHVHFICPQ LINEAIASGI
     TTLVGGGTGP AHGTCATTCT PAPSQMKLML QSTDEFPINV GFTGKGNTAK PEGLSEIIKA
     GAMGLKLHED WGSTPAAIDN CLSVAEAFDI QVNIHTDTLN EAGCVEHTIA AFKDRSIHTY
     HSEGAGGGHA PDIIKVCGVK NVLPSSTNPT RPFTSNTVDE HLDMLMVCHH LDKNIPEDVA
     FAESRIRAET IAAEDILHDM GAISIISSDS QAMGRIGEVI IRTWQTANKM KVQRGWLPGP
     GESDPAKYND NFRIRRYIAK YTINPAIVSG FSDFVGSVEA GKLADLVLWK PAFFGAKPEL
     IIKGGAIAWA NMGDPNASIP TPEPVMMRPM FGAYGKAGSS HSIAFVSKAA KEAGVASEYK
     LAKRVEAVGG VRSLTKLDMK LNDALPKIEV DPETYTVTAD GEVLTCQPAA TVPLSRNYFL
     F
//
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