ID M1DIW5_SOLTU Unreviewed; 547 AA.
AC M1DIW5;
DT 03-APR-2013, integrated into UniProtKB/TrEMBL.
DT 03-APR-2013, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE SubName: Full=Reticuline oxidase {ECO:0000313|EnsemblPlants:PGSC0003DMT400089776};
GN Name=102578664 {ECO:0000313|EnsemblPlants:PGSC0003DMT400089776};
OS Solanum tuberosum (Potato).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113 {ECO:0000313|EnsemblPlants:PGSC0003DMT400089776, ECO:0000313|Proteomes:UP000011115};
RN [1] {ECO:0000313|Proteomes:UP000011115}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DM1-3 516 R44 {ECO:0000313|Proteomes:UP000011115};
RX PubMed=21743474; DOI=10.1038/nature10158;
RG The Potato Genome Sequencing Consortium;
RT "Genome sequence and analysis of the tuber crop potato.";
RL Nature 475:189-195(2011).
RN [2] {ECO:0000313|EnsemblPlants:PGSC0003DMT400089776}
RP IDENTIFICATION.
RC STRAIN=DM1-3 516 R44 {ECO:0000313|EnsemblPlants:PGSC0003DMT400089776};
RG EnsemblPlants;
RL Submitted (JUN-2015) to UniProtKB.
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC {ECO:0000256|ARBA:ARBA00034114}.
CC -!- SIMILARITY: Belongs to the oxygen-dependent FAD-linked oxidoreductase
CC family. {ECO:0000256|ARBA:ARBA00005466}.
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DR RefSeq; XP_006353032.1; XM_006352970.2.
DR AlphaFoldDB; M1DIW5; -.
DR STRING; 4113.M1DIW5; -.
DR PaxDb; 4113-PGSC0003DMT400089776; -.
DR EnsemblPlants; PGSC0003DMT400089776; PGSC0003DMT400089776; PGSC0003DMG400039347.
DR GeneID; 102578664; -.
DR Gramene; PGSC0003DMT400089776; PGSC0003DMT400089776; PGSC0003DMG400039347.
DR KEGG; sot:102578664; -.
DR eggNOG; ENOG502QQWK; Eukaryota.
DR HOGENOM; CLU_018354_6_0_1; -.
DR InParanoid; M1DIW5; -.
DR OMA; ISMSYQA; -.
DR OrthoDB; 1094055at2759; -.
DR UniPathway; UPA00107; -.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-KW.
DR GO; GO:0071949; F:FAD binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009820; P:alkaloid metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0042179; P:nicotine biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.465.10; -; 1.
DR Gene3D; 3.40.462.20; -; 1.
DR Gene3D; 3.30.43.10; Uridine Diphospho-n-acetylenolpyruvylglucosamine Reductase, domain 2; 1.
DR InterPro; IPR012951; BBE.
DR InterPro; IPR016166; FAD-bd_PCMH.
DR InterPro; IPR036318; FAD-bd_PCMH-like_sf.
DR InterPro; IPR016167; FAD-bd_PCMH_sub1.
DR InterPro; IPR016169; FAD-bd_PCMH_sub2.
DR InterPro; IPR006094; Oxid_FAD_bind_N.
DR PANTHER; PTHR32448:SF59; FAD-BINDING PCMH-TYPE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR32448; OS08G0158400 PROTEIN; 1.
DR Pfam; PF08031; BBE; 1.
DR Pfam; PF01565; FAD_binding_4; 1.
DR SUPFAM; SSF56176; FAD-binding/transporter-associated domain-like; 1.
DR PROSITE; PS51387; FAD_PCMH; 1.
PE 3: Inferred from homology;
KW Alkaloid metabolism {ECO:0000256|ARBA:ARBA00022589};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Reference proteome {ECO:0000313|Proteomes:UP000011115};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..547
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004013890"
FT DOMAIN 76..254
FT /note="FAD-binding PCMH-type"
FT /evidence="ECO:0000259|PROSITE:PS51387"
SQ SEQUENCE 547 AA; 61160 MW; 5A77B11F925E332D CRC64;
MASSISMNIL FSFILLFSAS CVVSYTIQDQ FYQCITLHSD RSIPFSTAFF TPTSNATSFN
SVLKSTAQNL RCLTPSQQKP LLIFTALIES HVQAAVICAK ELKVQLRVRS GGHDYEGISY
TSEMKRSVSF ILLDLAKLRA IKVDIEDNSA WVQAGATIGE VYYRIAEKSK THGYPAGLCT
SLGIGGHITG GAYGPMMRKY GLGADNAVDA RIVDASGRVL DRALMGEDLF WAIRGGGGGS
FGIILAWKVR LVPVPSTVTV FTVPKTLETG ATKVLNKWQH VAHKIDEDLF IRILITTVNS
TKGKKTVQTA YQALFLGKTD RLLDVMNHSF PELGLTRKDC VEMSWIQSVI YIAGYPSNIK
PEFLLQGKSL FPTVNFKAKS DFLRVPVPVT GLQGMWEKFL QDDSPMMIWN PYGGMMGKIS
ESSIPFPHRK GVICKIQYLT SWTDGDKKTA DKHINWIRGL YEYMGTFVSK FPREAYVNYR
DLDLGMNKNA NSNFLEASVW GKKYYKNNYD RLVLVKTEVD PDNFFWHEQS LPILPFKVGQ
DGKSLIH
//
