ID M1MBM8_9CLOT Unreviewed; 672 AA.
AC M1MBM8;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=Cspa_c15590 {ECO:0000313|EMBL:AGF55329.1};
OS Clostridium saccharoperbutylacetonicum N1-4(HMT).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=931276 {ECO:0000313|EMBL:AGF55329.1, ECO:0000313|Proteomes:UP000011728};
RN [1] {ECO:0000313|EMBL:AGF55329.1, ECO:0000313|Proteomes:UP000011728}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N1-4(HMT) {ECO:0000313|Proteomes:UP000011728};
RA Poehlein A., Daniel R.;
RT "Genome sequence of Clostridium saccharoperbutylacetonicum N1-4(HMT).";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP004121; AGF55329.1; -; Genomic_DNA.
DR RefSeq; WP_015391651.1; NZ_AOIF01000129.1.
DR AlphaFoldDB; M1MBM8; -.
DR STRING; 36745.CLSAP_15220; -.
DR KEGG; csr:Cspa_c15590; -.
DR PATRIC; fig|931276.5.peg.1523; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_6_1_9; -.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000011728; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000011728}.
FT DOMAIN 3..273
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 274..540
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT COILED 470..528
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 24..31
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 672 AA; 78148 MW; 83BEC847454F7BB0 CRC64;
MGVNLDKYQM SAVKAEERNA LIVAAPGSGK TTVIINKVDH LVENKKVPNG NIIVITFTKA
AAQNMKNRYE AAFNKNRSPF FGTFHGLFYK ILLRSGKNIE IIDGSIVHKI VSNVLMKYFD
EINEDKVKEA VNNISLFKTS RVPLSEFKSS LAKEIFIEAL EKYEDYKKEN NKRDFDDLAI
EVLEMLQRDE NMLESYRKLF RYVLVDEFQD CDEMQVDFLK LINDGKENSL FAVGDEDQCI
YSFRGSKPEY MVSFDKIFKN GKKYYLSVNY RSKMNIVDKS KEIIRFNKER NNKEIHWNRE
AEGIIKWFNP YNEKMQGESL ADIIEKNRSL GTPYEDNAIL YRTNIESMTI IEVLAKRKIP
FTLLDREYNF FENFICKDLI AYLKLSINQF DLESFIQIIN KPFRYISKGN IAYIKNYAKE
ENPFDILISK EDIPPFQKKK FDDLRKEINY LNKISLSAAI SCIITDLGYM EHLKGYAKKL
NQSIEDLEDI IEEFKVAAEG SKTIFEFLSH IEEVKQSIEE SKKKTKREGV ILSTIHGVKG
MEFKNVYVIN CVEETIPHAN SIKDNIEEER RLFYVGVTRA IDNLYLFSPR NRKGQFKEVS
RFIIEGKLND LPVDTFGYEV GNKVAHRTYG VAEIAELDEK DNDKIKLKFG DGTFRSFSLK
VLVDNNLIER AE
//
