ID M2RD04_COCSN Unreviewed; 489 AA.
AC M2RD04;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=Vacuolar calcium ion transporter {ECO:0000256|RuleBase:RU365028};
GN ORFNames=COCSADRAFT_140739 {ECO:0000313|EMBL:EMD64684.1};
OS Cochliobolus sativus (strain ND90Pr / ATCC 201652) (Common root rot and
OS spot blotch fungus) (Bipolaris sorokiniana).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=665912 {ECO:0000313|EMBL:EMD64684.1, ECO:0000313|Proteomes:UP000016934};
RN [1] {ECO:0000313|EMBL:EMD64684.1, ECO:0000313|Proteomes:UP000016934}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ND90Pr / ATCC 201652 {ECO:0000313|Proteomes:UP000016934};
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [2] {ECO:0000313|Proteomes:UP000016934}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ND90Pr / ATCC 201652 {ECO:0000313|Proteomes:UP000016934};
RX PubMed=23357949; DOI=10.1371/journal.pgen.1003233;
RA Condon B.J., Leng Y., Wu D., Bushley K.E., Ohm R.A., Otillar R., Martin J.,
RA Schackwitz W., Grimwood J., MohdZainudin N., Xue C., Wang R., Manning V.A.,
RA Dhillon B., Tu Z.J., Steffenson B.J., Salamov A., Sun H., Lowry S.,
RA LaButti K., Han J., Copeland A., Lindquist E., Barry K., Schmutz J.,
RA Baker S.E., Ciuffetti L.M., Grigoriev I.V., Zhong S., Turgeon B.G.;
RT "Comparative genome structure, secondary metabolite, and effector coding
RT capacity across Cochliobolus pathogens.";
RL PLoS Genet. 9:E1003233-E1003233(2013).
CC -!- FUNCTION: Has a role in promoting intracellular calcium ion
CC sequestration via the exchange of calcium ions for hydrogen ions across
CC the vacuolar membrane. Involved also in manganese ion homeostasis via
CC its uptake into the vacuole. {ECO:0000256|RuleBase:RU365028}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|RuleBase:RU365028}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000256|ARBA:ARBA00008170, ECO:0000256|RuleBase:RU365028}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU365028}.
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DR EMBL; KB445642; EMD64684.1; -; Genomic_DNA.
DR RefSeq; XP_007699284.1; XM_007701094.1.
DR AlphaFoldDB; M2RD04; -.
DR GeneID; 19131176; -.
DR KEGG; bsc:COCSADRAFT_140739; -.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_1_1_1; -.
DR OMA; FQMRTHH; -.
DR OrthoDB; 276371at2759; -.
DR Proteomes; UP000016934; Unassembled WGS sequence.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1420.30; NCX, central ion-binding region; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR NCBIfam; TIGR00378; cax; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR PANTHER; PTHR31503:SF14; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|RuleBase:RU365028};
KW Calcium {ECO:0000256|RuleBase:RU365028};
KW Calcium transport {ECO:0000256|RuleBase:RU365028};
KW Ion transport {ECO:0000256|RuleBase:RU365028};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365028};
KW Reference proteome {ECO:0000313|Proteomes:UP000016934};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU365028};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU365028}; Transport {ECO:0000256|RuleBase:RU365028};
KW Vacuole {ECO:0000256|RuleBase:RU365028}.
FT TRANSMEM 118..139
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 151..172
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 184..205
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 226..244
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 264..282
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 314..338
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 383..409
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 415..436
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT DOMAIN 117..284
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT DOMAIN 320..460
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT REGION 1..72
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 489 AA; 53040 MW; 6D7D95A42FC0EE38 CRC64;
MAHSSRPSRD YPHAHTHGHG HRSSRDRAYS TDKPRSRRPS EDHRGRRRES LPQYNHNGHK
VSKGIHPEGE SGRSGFHPIK FLVISGKSSS TPSMLVNLLW PIVPIAIALH FVKPEWHLAV
FITNYIAMLP AANMLGFAGQ EFSRKMPNKS FAVVLETTFG SIVEIILFMV LLRTSKGDSN
VQVIKAAILG SILANLLLCL GSCFIAGGLK HNQQEFHEAI SENGSGLMLV AAMALVLPAV
FYSYLNQNPD YESNVDIAYN TRLISRGVAI ISIVGYLIYI VFQTVSHDGL LHEIYEEDDA
KDKDRHKELK KPKLTLIEVL VALVISLACV SLVAIALVQE IPYIVERGVS DAFVGLILIP
LVEKIAEHLL AIDEAYDNQI NMALAHVLGA SIQTALLNAP LVVLVGWGLG VGMDYNFSIF
DAVALVLAVL TVGSFLRDGK SNYLEGVLCV MTYIIIAVCA FYFPNPAGHG ASAGHGAAGG
AEGGAEGGH
//
