ID M2UF10_COCH5 Unreviewed; 1721 AA.
AC M2UF10;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 40.
DE RecName: Full=phosphoribosylformylglycinamidine synthase {ECO:0000256|ARBA:ARBA00012747};
DE EC=6.3.5.3 {ECO:0000256|ARBA:ARBA00012747};
DE AltName: Full=Formylglycinamide ribonucleotide amidotransferase {ECO:0000256|ARBA:ARBA00032632};
DE AltName: Full=Formylglycinamide ribotide amidotransferase {ECO:0000256|ARBA:ARBA00029823};
GN ORFNames=COCHEDRAFT_1193785 {ECO:0000313|EMBL:EMD92276.1};
OS Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) (Southern
OS corn leaf blight fungus) (Bipolaris maydis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=701091 {ECO:0000313|EMBL:EMD92276.1, ECO:0000313|Proteomes:UP000016936};
RN [1] {ECO:0000313|EMBL:EMD92276.1, ECO:0000313|Proteomes:UP000016936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O {ECO:0000313|Proteomes:UP000016936};
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [2] {ECO:0000313|Proteomes:UP000016936}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O {ECO:0000313|Proteomes:UP000016936};
RX PubMed=23357949; DOI=10.1371/journal.pgen.1003233;
RA Condon B.J., Leng Y., Wu D., Bushley K.E., Ohm R.A., Otillar R., Martin J.,
RA Schackwitz W., Grimwood J., MohdZainudin N., Xue C., Wang R., Manning V.A.,
RA Dhillon B., Tu Z.J., Steffenson B.J., Salamov A., Sun H., Lowry S.,
RA LaButti K., Han J., Copeland A., Lindquist E., Barry K., Schmutz J.,
RA Baker S.E., Ciuffetti L.M., Grigoriev I.V., Zhong S., Turgeon B.G.;
RT "Comparative genome structure, secondary metabolite, and effector coding
RT capacity across Cochliobolus pathogens.";
RL PLoS Genet. 9:E1003233-E1003233(2013).
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway; 5-
CC amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-
CC phospho-D-ribosyl)glycinamide: step 1/2.
CC {ECO:0000256|ARBA:ARBA00004920}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the FGAMS family.
CC {ECO:0000256|ARBA:ARBA00008608}.
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DR EMBL; KB445575; EMD92276.1; -; Genomic_DNA.
DR STRING; 701091.M2UF10; -.
DR eggNOG; KOG1907; Eukaryota.
DR eggNOG; KOG3007; Eukaryota.
DR HOGENOM; CLU_001031_0_1_1; -.
DR OMA; LSANWMW; -.
DR UniPathway; UPA00074; UER00128.
DR Proteomes; UP000016936; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004642; F:phosphoribosylformylglycinamidine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd01740; GATase1_FGAR_AT; 1.
DR CDD; cd02203; PurL_repeat1; 1.
DR CDD; cd02204; PurL_repeat2; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.30.1780.10; ornithine cyclodeaminase, domain 1; 1.
DR Gene3D; 1.10.8.750; Phosphoribosylformylglycinamidine synthase, linker domain; 1.
DR Gene3D; 3.90.650.10; PurM-like C-terminal domain; 2.
DR Gene3D; 3.30.1330.10; PurM-like, N-terminal domain; 2.
DR HAMAP; MF_00419; PurL_1; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR040707; FGAR-AT_N.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR003462; ODC_Mu_crystall.
DR InterPro; IPR023401; ODC_N.
DR InterPro; IPR010073; PurL_large.
DR InterPro; IPR041609; PurL_linker.
DR InterPro; IPR010918; PurM-like_C_dom.
DR InterPro; IPR036676; PurM-like_C_sf.
DR InterPro; IPR036921; PurM-like_N_sf.
DR InterPro; IPR036604; PurS-like_sf.
DR NCBIfam; TIGR01735; FGAM_synt; 1.
DR PANTHER; PTHR10099; PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE; 1.
DR PANTHER; PTHR10099:SF1; PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE; 1.
DR Pfam; PF02769; AIRS_C; 2.
DR Pfam; PF18072; FGAR-AT_linker; 1.
DR Pfam; PF18076; FGAR-AT_N; 1.
DR Pfam; PF13507; GATase_5; 1.
DR Pfam; PF02423; OCD_Mu_crystall; 1.
DR SMART; SM01211; GATase_5; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
DR SUPFAM; SSF109736; FGAM synthase PurL, linker domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR SUPFAM; SSF56042; PurM C-terminal domain-like; 2.
DR SUPFAM; SSF55326; PurM N-terminal domain-like; 2.
DR SUPFAM; SSF82697; PurS-like; 1.
DR PROSITE; PS51273; GATASE_TYPE_1; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962,
KW ECO:0000256|PROSITE-ProRule:PRU00605};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Purine biosynthesis {ECO:0000256|ARBA:ARBA00022755};
KW Reference proteome {ECO:0000313|Proteomes:UP000016936}.
FT DOMAIN 393..525
FT /note="Phosphoribosylformylglycinamidine synthase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF18076"
FT DOMAIN 548..598
FT /note="Phosphoribosylformylglycinamidine synthase linker"
FT /evidence="ECO:0000259|Pfam:PF18072"
FT DOMAIN 814..971
FT /note="PurM-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02769"
FT DOMAIN 1237..1365
FT /note="PurM-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02769"
FT ACT_SITE 1548
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 1683
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
FT ACT_SITE 1685
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00605"
SQ SEQUENCE 1721 AA; 187449 MW; B013E05E5EE94E96 CRC64;
MAFTVLSDND IRSLLCSLSP ADAEKLTSRL NQALSQYSCN DEAPYQPHRA QVTRPDGQVS
LFMPATTPSS IGVKIVGVAP SQAPPPGEKP RPALKSVLTI CDELGQAVGV LNAAELTAFR
TALGTMLLYR YRKFTQNIVV FGAGKQAEWH IRLAVLLKSN DISKITIVNR SRARADQLVE
TLTRAGLSSH VQIKVFEGGE DSLESLVKES QVMFCTTPST TPLFPASYLA SEADKPRFIS
AIGSYRLDMQ EIDPHLLSQI TTPRSLFASQ VHDACIAVDS IKGCMDEAGE LVKAGIATER
MIEVGKMDGL RQDNGAKRWL EQGFVVYKSV GVGVMDIAIG KALLELSGEK GVAHTMASTE
DPYLILPGSA AHSDFRLQRL AQAIGAKQVR SLWLHFVNPL KELADDELKT LQQILHYGEY
PDSNDRLAQT LLDAVHRGGE PRDGETVLFY VSPRAGTISP WSSLASMIAR TCTLDQAVKR
IERGMVIAAT FDRTLDADEI PNRDHLYDRM TQTISRTAPN LEAIFGEGEP AQATTISFDE
YNSAHAALDH ANRELGLAMD KSEIDYLVEA YTQELKRGPV DVELFMFAQV NSEHCRHKQF
NADFTVDGMR KSMSLFGMIR NTHQKNPQHV VSAYSDNAAV LQGEEASFWA PNDLTGEWNG
AKETVHILCK VETHNHPTAV SPFPGAATGS GGEIRDEGAV GRGSKPKAGL AGFTVSDLNL
EGFERPWELK DVGKPAHIAS SRDIMLEAPI GSAQFNNEFG RPCTVGYFRT MLMRVFTNEK
ESEIRGYHKP IMLAGGVGTV RPQHALKDPD VVPAGSHLLV IGGPAMLIGL GGGAASSIQS
GEGKVDLDFA SVQRGNPEVQ RRAQEVIDTC RSMGDKNPIL FIHDVGAGGL SNALPELVHD
SGLGAIFELR EVDSADKSMS PLQIWCCEAQ ERYVLAVAPD QLDLFKRICN RERCGYSVVG
TATKEQRLVL KDRDSKENPT PIDLPMATLF GKPPKMSRIV ESRKLRLPAF DSSLSMYIPD
TPKDGLISEA VDRVLTLPAV GSKSFLITIG DRTVGGLTVR DQMVGKWQTP VADVSVTATS
LLAGVKTGEA MTMGEKPTLA LISPVASARM AVAESLMNIA AASLFDRLSR VRLSANWMSA
SSHPGEGAAL YEAVEAIGMD LCPKLGISIP VGKDSMSMKM KWSEGGEAKE VTAPMSLVIT
AFAPVDRIDR TWTPALERVE SVGETILMYV DLAAGRKHLG GSALAQTFGQ VGDEAPDVYD
ADVIKDYFDA IDQLHESGIV LAYHDRSDGG LFTTLVEMAF AGRCGLEIML DRVAASSEPK
DVLEALFNEE LGAVFQVRKK DETAFNRCFA TCGPPPGMIK KIGRVPEASK QDISFYVGTK
NVYRNSRQKL QQRWAETSYR MQKLRDNPVC ADAEFNSILD DKDPGLSYNL TFKPSENIMP
LMSNLKSPFT AKPRVAILRE EGVNGQAEMA FAFHQAGFSA IDVHMTDIIS GRVSLAGFVG
LAACGGFSYG DVLGAGQGWA KSVLLHPETR KEFQDFFTRP NTFALGVCNG CQFMSKLKEL
IPGAELWPSF ERNASEQYEA RVCMVEVLDP KGTNTPPSVF LHGMAGSKLP IVTAHGEGRA
QFSSSASSAS TPQTLYNENL VSLRYVDNYG KQTETYPYNP NGSPMGITGV RSADGRVLAL
MPHPERTILK EVASYLPKNT EEWGELGPWS RMFKSARRWV G
//
