ID   M2XUJ0_9MICC            Unreviewed;       849 AA.
AC   M2XUJ0;
DT   01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2013, sequence version 1.
DT   27-MAR-2024, entry version 54.
DE   RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE            EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN   ORFNames=C884_00460 {ECO:0000313|EMBL:EME36473.1};
OS   Kocuria palustris PEL.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Micrococcaceae;
OC   Kocuria.
OX   NCBI_TaxID=1236550 {ECO:0000313|EMBL:EME36473.1, ECO:0000313|Proteomes:UP000009877};
RN   [1] {ECO:0000313|EMBL:EME36473.1, ECO:0000313|Proteomes:UP000009877}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PEL {ECO:0000313|EMBL:EME36473.1,
RC   ECO:0000313|Proteomes:UP000009877};
RX   PubMed=24504000;
RA   Sharma G., Khatri I., Subramanian S.;
RT   "Draft Genome Sequence of Kocuria palustris PEL.";
RL   Genome Announc. 2:e01261-13(2014).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-dependent breakage, passage and rejoining of double-
CC         stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC   -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC       family. {ECO:0000256|ARBA:ARBA00008263}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EME36473.1}.
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DR   EMBL; ANHZ02000014; EME36473.1; -; Genomic_DNA.
DR   RefSeq; WP_006214881.1; NZ_ANHZ02000014.1.
DR   AlphaFoldDB; M2XUJ0; -.
DR   STRING; 71999.KPaMU14_07020; -.
DR   Proteomes; UP000009877; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00187; TOP4c; 1.
DR   Gene3D; 3.30.1360.40; -; 1.
DR   Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR   Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 1.
DR   Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR   InterPro; IPR006691; GyrA/parC_rep.
DR   InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR   InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR   InterPro; IPR013758; Topo_IIA_A/C_ab.
DR   InterPro; IPR013757; Topo_IIA_A_a_sf.
DR   InterPro; IPR002205; Topo_IIA_dom_A.
DR   PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR   Pfam; PF03989; DNA_gyraseA_C; 2.
DR   Pfam; PF00521; DNA_topoisoIV; 1.
DR   SMART; SM00434; TOP4c; 1.
DR   SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR   SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:EME36473.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000009877};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          27..480
FT                   /note="DNA topoisomerase type IIA"
FT                   /evidence="ECO:0000259|SMART:SM00434"
FT   REGION          808..849
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          445..479
FT                   /evidence="ECO:0000256|SAM:Coils"
SQ   SEQUENCE   849 AA;  91595 MW;  5C1F1B3363E659CB CRC64;
     MAPRRSKAQK SALDELPADF EGNVVDIDVT REMEDSFLEY SYSVIYSRAL PDARDGLKPV
     QRRILYMMDE MGLRPDRGHV KSARVVGEVM GKLHPHGDSA IYDAMVRMAL PFAQRMPLVD
     GHGNFGSLDD GPAASRYTEA RLAPAALQLT ADLGEDVVDF VPNYDNQFMQ PSVLPAAYPN
     LLVNGTTGIA VGMATNMAPH NLREAVAGAR HLLEHPEASL EDVMKHIPGP DLPSGGQIVG
     LDGIRDAYRT GRGSFKTRAA VSIEQVTPRK QGIVVTELPY MVGPEKVIDR IKSAVQSKKL
     TGISDVVDLT DRTKGLNLVI EIKNGFNPQA VLAALYKHTP LEESFGINNV ALVDGRPQTL
     GLLEMLRVYN QHRLTVVRRR TAHRLGKRRD RLHLVEGLLI AIVDIDEVIQ IIRSSDQTAE
     ARERLMTIFD LTQLQADHIL ELRLRQLTKF SRIELERERE ELEREIAELE AILASEDRLR
     EVVSGELAAV AELLGDDRRT RLLEADEAPA LLAAPAAKAA AGPALEIADD PCWVFLSSTG
     RMARTNDRTP LAEPGRRLKH DALRSVVATS ARGEIGALTS SGRMIRVPVM DLPALSSYDG
     APRLEEGTAA KDFLSLAKGE RLIGLAALNE VVAMGTRQGV VKRLNGDHPL NRDDWEVISL
     KDGDEVVGMG PAPDSAQLVF ITAQAKLLTF SAELVRAQGR TAAGVAGIKL ADDDSVVFFG
     PVASQDPDAR VVTYARSSEA LPKMGGYSVK VTPLSEYPSK GRATAGVRCQ RLLSGETGLV
     LAWAGHGPAK AVSAAGVARS LPTEFGRRDG SGVGVEQLPD AIGPAWSPVS SAASAAEGAD
     GDAESEEQA
//