ID M2XVR2_GALSU Unreviewed; 943 AA.
AC M2XVR2;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE SubName: Full=tRNA pseudouridine synthase A {ECO:0000313|EMBL:EME27504.1};
DE EC=5.4.99.12 {ECO:0000313|EMBL:EME27504.1};
GN ORFNames=Gasu_49530 {ECO:0000313|EMBL:EME27504.1};
OS Galdieria sulphuraria (Red alga).
OC Eukaryota; Rhodophyta; Bangiophyceae; Galdieriales; Galdieriaceae;
OC Galdieria.
OX NCBI_TaxID=130081 {ECO:0000313|EMBL:EME27504.1, ECO:0000313|Proteomes:UP000030680};
RN [1] {ECO:0000313|Proteomes:UP000030680}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=074W {ECO:0000313|Proteomes:UP000030680};
RX PubMed=23471408; DOI=10.1126/science.1231707;
RA Schonknecht G., Chen W.H., Ternes C.M., Barbier G.G., Shrestha R.P.,
RA Stanke M., Brautigam A., Baker B.J., Banfield J.F., Garavito R.M., Carr K.,
RA Wilkerson C., Rensing S.A., Gagneul D., Dickenson N.E., Oesterhelt C.,
RA Lercher M.J., Weber A.P.;
RT "Gene transfer from bacteria and archaea facilitated evolution of an
RT extremophilic eukaryote.";
RL Science 339:1207-1210(2013).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; KB454531; EME27504.1; -; Genomic_DNA.
DR RefSeq; XP_005704024.1; XM_005703967.1.
DR AlphaFoldDB; M2XVR2; -.
DR STRING; 130081.M2XVR2; -.
DR EnsemblPlants; EME27504; EME27504; Gasu_49530.
DR GeneID; 17086407; -.
DR Gramene; EME27504; EME27504; Gasu_49530.
DR KEGG; gsl:Gasu_49530; -.
DR eggNOG; KOG2553; Eukaryota.
DR OrthoDB; 12038at2759; -.
DR Proteomes; UP000030680; Unassembled WGS sequence.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:EME27504.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000030680};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 246..350
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT ACT_SITE 144
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 205
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 943 AA; 109664 MW; 52E854DE7D8B887A CRC64;
MGLFWSHPQV LHNDRVLKRN RALFDTTENK VPFKKSKGIE NESLVTTTTK KDNLLLASAE
ESVSVKTSVC NSKTVAHNSN SKKRHKFAIV IGYLGAAYQG MQRNPGAVSI EGELEKALVA
TGLIPNNLEG NLRKIEWSRA ARTDKGVSAA GQVVSTYLLV DKDFEREQDI LKRVNDTLPT
DIRILGVYSV SGGFHAKNAC DRRRYSYMFP SWTLCRSKQE HEKMLKDSDY KNIMKAKVDD
LNDLLKYFIG THHFHNFTVG QTSSNGTCQR YILEFFCSLP FFIGGESFLN VTVVGQSFLL
HQIRKMIGLA IAVSKGWIPK EAFNVSLSKH HRLETPMAPS LGLFLDECLF ERYNERILKN
HSDSPAVEEL ARIRQRAELF KRQIIYEYIA CQEREHHVME RWMFLVSTKH PVDVSSIMTK
HEELMNQEII LDRKRKEYML DHISPLFDSS GIYGQHEVTL PSTLDERLNR CIEALAPKSI
SFYFRAPLES ICIMGELTKS LKVPCVYLTS GKDIIWGISV EEGNFSITLY DSRSPSKPCY
TMDRNGSLEE SFVSYDYFCF GWKVANEFFQ CEEIPHKKIS IVFDSVHDNR CKMEIAYYCS
AFFIFMKAMR PKKSLSKQEI AEILIQGMIS RSLDIPSTEI YSCICSRANH LLVIRPIMET
QKVAIEHFCV PQWLFEQMKW LEESAVLEEN NHRQIKALWE DWLQKVHIIG ALELKKSDLN
WISQTKDEIP TQAHSPGMLL WKKKGSNHRV EYPERNEERH FFLDSIHEWK ASPLEIANIL
NISWENFFSL FGEIDMEERV EWKFYLNEFI NQVNSIDSFL VNWKNMVEKD DMILMEKMKL
LERCLDNLRD SQKLLQLWFR KLSFSWFTAT GCVQYCIQPN AIIEWKPLAL VPSDWTGNDK
VCEKASLHPS SGICFWYPHF VKFVRWSSNR RKVRKSARKY RLR
//
