ID   M3AXB4_PSEFD            Unreviewed;       808 AA.
AC   M3AXB4;
DT   01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2013, sequence version 1.
DT   24-JAN-2024, entry version 46.
DE   RecName: Full=cysteine--tRNA ligase {ECO:0000256|ARBA:ARBA00012832};
DE            EC=6.1.1.16 {ECO:0000256|ARBA:ARBA00012832};
DE   AltName: Full=Cysteinyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031499};
GN   ORFNames=MYCFIDRAFT_203918 {ECO:0000313|EMBL:EME82117.1};
OS   Pseudocercospora fijiensis (strain CIRAD86) (Black leaf streak disease
OS   fungus) (Mycosphaerella fijiensis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Pseudocercospora.
OX   NCBI_TaxID=383855 {ECO:0000313|EMBL:EME82117.1, ECO:0000313|Proteomes:UP000016932};
RN   [1] {ECO:0000313|EMBL:EME82117.1, ECO:0000313|Proteomes:UP000016932}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CIRAD86 {ECO:0000313|EMBL:EME82117.1,
RC   ECO:0000313|Proteomes:UP000016932};
RX   PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA   Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA   Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA   LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA   Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA   Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA   Grigoriev I.V.;
RT   "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT   genomes of eighteen Dothideomycetes fungi.";
RL   PLoS Pathog. 8:E1003037-E1003037(2012).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000256|ARBA:ARBA00001947};
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DR   EMBL; KB446559; EME82117.1; -; Genomic_DNA.
DR   RefSeq; XP_007927553.1; XM_007929362.1.
DR   AlphaFoldDB; M3AXB4; -.
DR   STRING; 383855.M3AXB4; -.
DR   GeneID; 19336207; -.
DR   KEGG; pfj:MYCFIDRAFT_203918; -.
DR   eggNOG; KOG2007; Eukaryota.
DR   HOGENOM; CLU_013528_3_1_1; -.
DR   OrthoDB; 2140072at2759; -.
DR   Proteomes; UP000016932; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004817; F:cysteine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00672; CysRS_core; 1.
DR   Gene3D; 3.40.50.620; HUPs; 2.
DR   HAMAP; MF_00041; Cys_tRNA_synth; 1.
DR   InterPro; IPR015803; Cys-tRNA-ligase.
DR   InterPro; IPR024909; Cys-tRNA/MSH_ligase.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR032678; tRNA-synt_1_cat_dom.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   NCBIfam; TIGR00435; cysS; 1.
DR   PANTHER; PTHR10890:SF3; CYSTEINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR   PANTHER; PTHR10890; CYSTEINYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW   Reference proteome {ECO:0000313|Proteomes:UP000016932};
KW   Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT   DOMAIN          45..480
FT                   /note="tRNA synthetases class I catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF01406"
FT   REGION          707..747
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        709..747
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   808 AA;  91142 MW;  C12B7EADF7486990 CRC64;
     MAAAQPEWRA PPAPSAEVQA RLPKLSVYNS LTRTKTPFIP LDKSGKKVGW YACGPTVYDD
     SHLGHARNYV SQDIIRRIMS DYLGFHINFV MNITDVDDKI ILAAREQYLL AEFLQGRKEV
     DDELRQTTSE ALKEYASKKV PKLESYLPES SHADDAAVAK YTQGERTAYQ NVLDGKSEDG
     QGAPGDKEAK IKMHLKTVKN AVDALHSSDA TLSLFVEKAS GVLRPYIDRL NSSKVSGKDH
     HIFTKLTKKY EQRFFDDMKA LNVRYPDKLT RVTEYGPQIV DFVKQIQGNG YAYENEGSVY
     YDTTAWESSG GHYARLEPWN RNDSELQADG EGSLAAKKTS FKKSGADFAL WKASKAGEPS
     WDSPWGEGRP GWHIECSAMA SDVLGKQFDI HSGGIDLAFP HHDNELAQSE AYWHKCGAES
     EQWVNYFLHM GHLSISGSKM SKSLKNFTTI KEALSKGDFT PRSLRIIYLL GNWRDGLEIT
     PDLRKASVAW EERVDNFFLK VRDLKKNPSS DYKNGTNGTQ PDIGQLLEGS MEKFREAILD
     SFDTPAAMRV LSELITAYNS AQHVPDATSE AIGEWITQMV VMFGLDASYR KGQMGWSGID
     IPEKAKPFIE PLSELRDKVR EQAIKGQVEL ESLGRLEEKD NFYRAQEQPY AKAYADFQRK
     VFELHSKSAP AKEYLAACDD LRDSVLWSLG IYLEDRENRP ALVRPLSESL KQERQERESK
     AAEKAAAKEK AKAEAAAKEK ERLEKGKIPP AELFKTEEYK AIYSEWDDKG MPTKDKEGKE
     VAKSQVKKLA KAYENQKKLH EAWLAAQK
//