ID   M3H4N2_LEPBO            Unreviewed;       648 AA.
AC   M3H4N2;
DT   01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2013, sequence version 1.
DT   27-MAR-2024, entry version 34.
DE   SubName: Full=Penicillin-binding protein 2 {ECO:0000313|EMBL:EMG02019.1};
GN   Name=mrdA {ECO:0000313|EMBL:EMG02019.1};
GN   ORFNames=LEP1GSC123_0202 {ECO:0000313|EMBL:EMG02019.1};
OS   Leptospira borgpetersenii str. 200701203.
OC   Bacteria; Spirochaetota; Spirochaetia; Leptospirales; Leptospiraceae;
OC   Leptospira.
OX   NCBI_TaxID=1193007 {ECO:0000313|EMBL:EMG02019.1, ECO:0000313|Proteomes:UP000011783};
RN   [1] {ECO:0000313|EMBL:EMG02019.1, ECO:0000313|Proteomes:UP000011783}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=200701203 {ECO:0000313|EMBL:EMG02019.1,
RC   ECO:0000313|Proteomes:UP000011783};
RA   Harkins D.M., Durkin A.S., Brinkac L.M., Haft D.H., Selengut J.D.,
RA   Sanka R., DePew J., Purushe J., Picardeau M., Werts C., Goarant C.,
RA   Vinetz J.M., Sutton G.G., Nierman W.C., Fouts D.E.;
RL   Submitted (JAN-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004167}; Single-
CC       pass membrane protein {ECO:0000256|ARBA:ARBA00004167}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EMG02019.1}.
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DR   EMBL; AKWO02000010; EMG02019.1; -; Genomic_DNA.
DR   AlphaFoldDB; M3H4N2; -.
DR   Proteomes; UP000011783; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; IEA:InterPro.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR   Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   InterPro; IPR017790; Penicillin-binding_protein_2.
DR   NCBIfam; TIGR03423; pbp2_mrdA; 1.
DR   PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR   PANTHER; PTHR30627:SF2; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE MRDA; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE   4: Predicted;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000011783};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        21..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          64..244
FT                   /note="Penicillin-binding protein dimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF03717"
FT   DOMAIN          277..615
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
FT   REGION          627..648
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        628..648
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   648 AA;  71131 MW;  27F54FA15A022E1D CRC64;
     MFGGAQSASE YRLEKSFRLR LYIFSGLVVF TLAAFIFQLF NLQILNGTSN ALKAEKFVRK
     SETMPAARGQ MFDRNFLTPE TSMALVSNYS SLDAILNTSL FKYDPASVKA FIQEFARTLS
     IPISYYENEL IEPRFSRNLK AKKPIVLLEG ITHAQQERIS AFDNISKYII LLPSPRRIYK
     MGPALAHVTG YIGKPTREDL LSGEIKSYQY LGKSGLELEY DSELRGVDGF RIQKRSSEGN
     IEEERVVEHS SPGNNLILTI DKDIQIAAHK ALKGSRGTAI AIKVSTGEIL AMVSNPSYDP
     NILSGKNKAD RTAHYNRVNS NGGFLNLAIQ SKFPPASTYK TLVALAALES QHKIGYTPET
     TFSCNGSFVL KSTFAGVPDQ VFNCWEKGGH GINDLAHALQ KSCSVYFYNL GYKLGSDAIL
     NYSRLFGLED KSKVDLPGEI TGFVPSSAWK KRTYGTKWFD GDTINLSIGQ GFISTTPMGM
     ALFYMGLLNR GQIYQPYVVS EIRDPVDNSI INRTTPQILR DIPINQSSAE AIKEGLKLVI
     KSGTAAYVLN KPFLPDIAGK TGTAQTRRRG SSGSNHAWFV GYAPANAPVS EQVLVVVFVE
     YGVGGAAGAA PVAREMFHAA FPPGTFKKSQ DITPSSPSTG QEGTFEAR
//