ID M3V5R5_9ACTN Unreviewed; 484 AA.
AC M3V5R5;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=diacylglycerol O-acyltransferase {ECO:0000256|ARBA:ARBA00013244};
DE EC=2.3.1.20 {ECO:0000256|ARBA:ARBA00013244};
GN ORFNames=GP2_030_00060 {ECO:0000313|EMBL:GAC85117.1};
OS Gordonia paraffinivorans NBRC 108238.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1223543 {ECO:0000313|EMBL:GAC85117.1, ECO:0000313|Proteomes:UP000035021};
RN [1] {ECO:0000313|EMBL:GAC85117.1, ECO:0000313|Proteomes:UP000035021}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 108238 {ECO:0000313|EMBL:GAC85117.1,
RC ECO:0000313|Proteomes:UP000035021};
RA Isaki-Nakamura S., Hosoyama A., Tsuchikane K., Ando Y., Baba S., Ohji S.,
RA Hamada M., Tamura T., Yamazoe A., Yamazaki S., Fujita N.;
RT "Whole genome shotgun sequence of Gordonia paraffinivorans NBRC 108238.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAC85117.1}.
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DR EMBL; BAOQ01000030; GAC85117.1; -; Genomic_DNA.
DR RefSeq; WP_006901342.1; NZ_BAOQ01000030.1.
DR AlphaFoldDB; M3V5R5; -.
DR STRING; 1223543.GP2_030_00060; -.
DR eggNOG; COG1020; Bacteria.
DR OrthoDB; 9810950at2; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000035021; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 23..288
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 327..465
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 484 AA; 52982 MW; 08F292AE5A5C823E CRC64;
MSNDADLTHG VDEPLDWARD PHMSPVDTIM WRGDTDRRMR GTICMLEIYD CDPDWDRLVA
AHEWASRMAP RFRQRVVDSP FGTGTPSWAV DPDFDLHYHL RRVRLGGDGS MRELLTTCEQ
LAMTALDAAR PPWEGYLFEG LPDGKAAYFL KAHHALTDGL GAIIGLAQLH SPTREHNPKK
PQPPSPDPVN ITTLDILERQ VAEEIRRVPH RIDLTLKGIR ALAHPREALS TALRYGRSVP
RVAGLVSPPG SPLLANRSVS WRFGAFEVPF ADLKAAATAA RSSVNDVYLA GLIGGFRYYH
EKMNCPVEAI PVAIPISVRR PEDPAGGNRI AVGRLAGPVG IADPFERVLT IREQVRQARH
EPAVDIFNTL GSVMAWLPGS VLMRFSGATS QNDLQASNVP GIPWDTYVAG AKVERMFPFG
PLPGCAMMAT MITHNGVACL GINSDGAAVT DQELWAECLV AGFNEVLALA ADDNRPPGQL
KRVV
//
