ID M3WLK3_FELCA Unreviewed; 2173 AA.
AC M3WLK3;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 28-FEB-2018, sequence version 2.
DT 24-JAN-2024, entry version 65.
DE RecName: Full=Mediator of RNA polymerase II transcription subunit 13 {ECO:0000256|RuleBase:RU364134};
GN Name=MED13 {ECO:0000313|Ensembl:ENSFCAP00000013826.4,
GN ECO:0000313|VGNC:VGNC:63433};
OS Felis catus (Cat) (Felis silvestris catus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX NCBI_TaxID=9685 {ECO:0000313|Ensembl:ENSFCAP00000013826.4, ECO:0000313|Proteomes:UP000011712};
RN [1] {ECO:0000313|Ensembl:ENSFCAP00000013826.4, ECO:0000313|Proteomes:UP000011712}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000013826.4,
RC ECO:0000313|Proteomes:UP000011712};
RX PubMed=17975172; DOI=10.1101/gr.6380007;
RA Pontius J.U., Mullikin J.C., Smith D.R., Lindblad-Toh K., Gnerre S.,
RA Clamp M., Chang J., Stephens R., Neelam B., Volfovsky N., Schaffer A.A.,
RA Agarwala R., Narfstrom K., Murphy W.J., Giger U., Roca A.L., Antunes A.,
RA Menotti-Raymond M., Yuhki N., Pecon-Slattery J., Johnson W.E., Bourque G.,
RA Tesler G., O'Brien S.J.;
RT "Initial sequence and comparative analysis of the cat genome.";
RL Genome Res. 17:1675-1689(2007).
RN [2] {ECO:0000313|Ensembl:ENSFCAP00000013826.4, ECO:0000313|Proteomes:UP000011712}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000013826.4,
RC ECO:0000313|Proteomes:UP000011712};
RA Hillier L.W., Warren W., Obrien S., Wilson R.K.;
RT "Sequence assembly of the Felis catus genome version 6.2.";
RL Submitted (SEP-2011) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Ensembl:ENSFCAP00000013826.4}
RP IDENTIFICATION.
RC STRAIN=breed Abyssinian {ECO:0000313|Ensembl:ENSFCAP00000013826.4};
RG Ensembl;
RL Submitted (SEP-2023) to UniProtKB.
CC -!- FUNCTION: Component of the Mediator complex, a coactivator involved in
CC the regulated transcription of nearly all RNA polymerase II-dependent
CC genes. Mediator functions as a bridge to convey information from gene-
CC specific regulatory proteins to the basal RNA polymerase II
CC transcription machinery. Mediator is recruited to promoters by direct
CC interactions with regulatory proteins and serves as a scaffold for the
CC assembly of a functional preinitiation complex with RNA polymerase II
CC and the general transcription factors. {ECO:0000256|RuleBase:RU364134}.
CC -!- SUBUNIT: Component of the Mediator complex, which is composed of MED1,
CC MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L,
CC MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23,
CC MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and
CC CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct
CC module termed the CDK8 module. Mediator containing the CDK8 module is
CC less active than Mediator lacking this module in supporting
CC transcriptional activation. Individual preparations of the Mediator
CC complex lacking one or more distinct subunits have been variously
CC termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.
CC {ECO:0000256|RuleBase:RU364134}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123,
CC ECO:0000256|RuleBase:RU364134}.
CC -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC {ECO:0000256|ARBA:ARBA00009354, ECO:0000256|RuleBase:RU364134}.
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DR EMBL; AANG04003358; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_011287389.1; XM_011289087.2.
DR STRING; 9685.ENSFCAP00000013826; -.
DR PaxDb; 9685-ENSFCAP00000013826; -.
DR Ensembl; ENSFCAT00000014909.6; ENSFCAP00000013826.4; ENSFCAG00000014905.6.
DR GeneID; 101091826; -.
DR KEGG; fca:101091826; -.
DR CTD; 9969; -.
DR VGNC; VGNC:63433; MED13.
DR eggNOG; KOG3600; Eukaryota.
DR GeneTree; ENSGT00390000013680; -.
DR HOGENOM; CLU_000508_0_0_1; -.
DR InParanoid; M3WLK3; -.
DR OMA; WWGEDPS; -.
DR OrthoDB; 9289at2759; -.
DR Proteomes; UP000011712; Chromosome E1.
DR Bgee; ENSFCAG00000014905; Expressed in embryonic head and 10 other cell types or tissues.
DR GO; GO:1990508; C:CKM complex; IEA:Ensembl.
DR GO; GO:0016592; C:mediator complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0046966; F:nuclear thyroid hormone receptor binding; IEA:Ensembl.
DR GO; GO:0003713; F:transcription coactivator activity; IBA:GO_Central.
DR GO; GO:0042632; P:cholesterol homeostasis; IEA:Ensembl.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060261; P:positive regulation of transcription initiation by RNA polymerase II; IEA:Ensembl.
DR GO; GO:0070328; P:triglyceride homeostasis; IEA:Ensembl.
DR InterPro; IPR009401; Med13_C.
DR InterPro; IPR041285; MID_MedPIWI.
DR PANTHER; PTHR48249; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13; 1.
DR PANTHER; PTHR48249:SF4; MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 13; 1.
DR Pfam; PF06333; Med13_C; 1.
DR Pfam; PF18296; MID_MedPIWI; 1.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|RuleBase:RU364134};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU364134};
KW Reference proteome {ECO:0000313|Proteomes:UP000011712};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|RuleBase:RU364134};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU364134};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|RuleBase:RU364134}.
FT DOMAIN 1366..1723
FT /note="MID"
FT /evidence="ECO:0000259|Pfam:PF18296"
FT DOMAIN 1759..2162
FT /note="Mediator complex subunit Med13 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06333"
FT REGION 318..341
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 710..736
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 749..769
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 787..816
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 961..1053
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1483..1506
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1532..1628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2014..2044
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 428..455
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 456..473
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 710..731
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 753..769
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 972..1002
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1005..1019
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1031..1053
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1532..1611
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2173 AA; 239493 MW; 18473DA249A03A09 CRC64;
MSSSFVPNGA SLEDCHCNLF CLADLTGIKW KRYVWQGPTS APILFPVTEE DPILSSFSRC
LKADVLGVWR RDQRPGRREL WIFWWGEDPN FADLIHHDLS EEEDGVWENG LSYECRTLLF
KAVHNLLERC LMNRNFVRIG KWFVKPYEKD EKPINKSEHL SCSFTFFLHG DSNVCTSVEI
NQHQPVYLLS EEHITLAQQS NSPFQVILSP FGLNGTLTGQ AFKMSDSATK KLIGEWKQFY
PISSCLKEMS EEKQEDMDWE DDSLAAVEVL VAGVRMIYPA CFVLVPQSDI PTPSSVGSSH
CSASCLGVHQ VPASTRDPAM SSVTLTPPTS PEEVQTVDPQ SAQKWVKFSS VSDGFNSDST
SHHGGKIPRK LANHVVDRVW QECNMNRAQN KRKYSASSGG LSEEEIPDKI ASWDFVEATQ
RTNCSCLRHK NLKPRNPGQQ GQAPSLSQQQ QVLPKHKTNE KQEKSEKPQK RPLTPFHHRV
SISDDVGIDA DSASQRLVIS APDSQVRFSN IRTNDVAKTP QMHGTEMANS PQPPPLSPHP
CDVVDEGVTK TPSTPQSQHF YQMPTPDPLV PSKPMEDRID SLSQSFPAQF QEAVEPTIYV
GTAVNLEEDE ANIAWKYYKV PKKKDVEFLP PQLPSDKFKD DPVGPFGQES VTSVTELMVQ
CKKPLKVSDE LVQQYQIKNQ YLSAIASDAE QESKIDPYAF VEGDEEFLFP DKKDRQNSER
EAVKKHKVED GPSSVTVLSH EEDAMSLFSP SIKQDAPRPT SHARPPSTSL IYDSDLAVSY
TDLDNLFNSD EDELTPGSKK SANGTDDKSS CKESKTGNLD PLSCISTADL HKMYPTPPSL
EQHIMGFSPM NMSNKEYGSM DITPGGTVLE GNSSTVGTQF KIEVDEGFCS PKPSEIKDFS
YVYKPENCQV LVGCSMFAPL KSLPSQCLPP IKLPEECIYR QSWTVGKLEL LSSGPAVPFI
KEGDGSTIDQ EYGPAYTPQT HTSFGMPPSS APPSNSGAGI LPSPSTPRFP TPRTPRTPRT
PRAGGPASAQ GSVKYENSDL YSPASTPSTC RPLNSVEPAT VPSIPEAHSL YVNLILSESV
MNLFKDCNFD SCCICVCNMN IKGADVGVYI PDPMQEAQYR CTCGFSAVMN RKFGNNSGLF
LEDELDIIGR NTECGKEAEK RFEALRATSA EHVNGGLKES EKFPDELILL LQDQCTNLFS
PFGAADQDPF PRIGVISNWV RVEERDCCND CYLALEHGRQ FMDNMSGGKV DEALVKSSCL
HPWSKRNDVS MQCSQDILRM LLSLQPVLQD AIQKKRTVRP WGVQGPLTWQ QFHKMAGRGS
YGTDESPEPL PIPTFLLGYD YDFLVLSPFA LPYWERLMLE PYGSQRDIAY VVLCPENEAL
LNGAKSFFRD LTAIYESCRL GQHRPISRLL TDGIMRVGST ASKKLSEKLV AEWFSQAADG
NNEAFSKLKL YAQVCRYDLG PYLASQPLDS SLLSQPNLVA PTSQSLITPP QMTNTGNANT
PSATLASAAS STMTMTSGVA MSSSVATANS TLTTTSTSSS SSSNLSSGVP SNKLPSFPPF
GSMNSSATGS MSTQASTVQN GQLGGQQSSA LQTAGISGES SSLPTQPHPD VSESTMDRDK
VGIPTDGDSH AITYPPAIVV YIIDPFTYEN KDESTNSSNV WTLGLLRCFL EMVQTLPPHI
KSTVSVQIVP CQYLLQPVKH EDRQIYTQHL KSLAFSAFTQ CRRPLPTSTN VKTLTGFGPG
LAMETALKSP DRPECIRLYT PPFILAPVKD KQTELGETFG EAGQKYNVLF VGYCLSHDQR
WILASCTDLY GELLETCIIN IDVPNRARRK KGSARRFGLQ KLWEWCLGLV QMSSLPWRVV
IGRLGRIGHG ELKDWSSLLS RRNLQSLSKR LKDMCRMCGI SAADSPSILS ACLVAMEPQG
SFVIMPDSVS TGSVFGRSTT LNMQTSQLNT PQDTSCTHIL VFPTSASVQV ASATYTTENL
DLAFNPNNDG ADGMGIFDLL DTGDDLDPDI INILPASPTG SPVHSPGSHY PHGGDAGKGQ
GTDRLLSTES HDEVTNILQQ PLALGYFVST AKAGPLPDWF WSACPQAQYQ CPLFLKASLH
LHVPSVQSDE LLHSKHSHPL DSNQTSDVLR FVLEQYNALS WLTCDPAIQD RRSCLPIHFV
VLNQLYNFIM NML
//
