ID M3YJ50_MUSPF Unreviewed; 1250 AA.
AC M3YJ50;
DT 01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 01-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=Apoptotic protease-activating factor 1 {ECO:0000256|PIRNR:PIRNR037646};
DE Short=APAF-1 {ECO:0000256|PIRNR:PIRNR037646};
GN Name=APAF1 {ECO:0000313|Ensembl:ENSMPUP00000011357.1,
GN ECO:0000313|RefSeq:XP_004742988.1, ECO:0000313|RefSeq:XP_004742989.1,
GN ECO:0000313|RefSeq:XP_004742990.1, ECO:0000313|RefSeq:XP_004742992.1};
OS Mustela putorius furo (European domestic ferret) (Mustela furo).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Musteloidea; Mustelidae;
OC Mustelinae; Mustela.
OX NCBI_TaxID=9669 {ECO:0000313|Ensembl:ENSMPUP00000011357.1};
RN [1] {ECO:0000313|Ensembl:ENSMPUP00000011357.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAR-2023) to UniProtKB.
RN [2] {ECO:0000313|RefSeq:XP_004742988.1, ECO:0000313|RefSeq:XP_004742989.1}
RP IDENTIFICATION.
RC TISSUE=Brain {ECO:0000313|RefSeq:XP_004742988.1,
RC ECO:0000313|RefSeq:XP_004742989.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent
CC autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the
CC activation of caspase-3 and apoptosis. This activation requires ATP.
CC {ECO:0000256|PIRNR:PIRNR037646}.
CC -!- SUBUNIT: Monomer. Oligomerizes upon binding of cytochrome c and dATP.
CC {ECO:0000256|PIRNR:PIRNR037646}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR037646}.
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DR EMBL; AEYP01012181; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012182; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012183; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012184; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012185; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012186; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012187; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AEYP01012188; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_004742988.1; XM_004742931.3.
DR RefSeq; XP_004742989.1; XM_004742932.3.
DR RefSeq; XP_004742990.1; XM_004742933.3.
DR RefSeq; XP_004742991.1; XM_004742934.2.
DR RefSeq; XP_004742992.1; XM_004742935.3.
DR RefSeq; XP_012906957.1; XM_013051503.1.
DR STRING; 9669.ENSMPUP00000011357; -.
DR Ensembl; ENSMPUT00000011545.1; ENSMPUP00000011357.1; ENSMPUG00000011447.1.
DR GeneID; 101691091; -.
DR KEGG; mpuf:101691091; -.
DR CTD; 317; -.
DR eggNOG; KOG4155; Eukaryota.
DR eggNOG; KOG4658; Eukaryota.
DR GeneTree; ENSGT00940000157710; -.
DR HOGENOM; CLU_005071_1_0_1; -.
DR OMA; YHMANAN; -.
DR OrthoDB; 2995530at2759; -.
DR Proteomes; UP000000715; Unplaced.
DR GO; GO:0043293; C:apoptosome; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; IEA:Ensembl.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008656; F:cysteine-type endopeptidase activator activity involved in apoptotic process; IEA:Ensembl.
DR GO; GO:0042802; F:identical protein binding; IEA:Ensembl.
DR GO; GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0030900; P:forebrain development; IEA:Ensembl.
DR GO; GO:0070059; P:intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress; IEA:Ensembl.
DR GO; GO:0001843; P:neural tube closure; IEA:Ensembl.
DR GO; GO:0051402; P:neuron apoptotic process; IEA:Ensembl.
DR GO; GO:2001235; P:positive regulation of apoptotic signaling pathway; IEA:Ensembl.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:1902510; P:regulation of apoptotic DNA fragmentation; IEA:Ensembl.
DR CDD; cd08323; CARD_APAF1; 1.
DR CDD; cd00200; WD40; 2.
DR Gene3D; 1.25.40.370; -; 1.
DR Gene3D; 1.10.533.10; Death Domain, Fas; 1.
DR Gene3D; 1.10.8.430; Helical domain of apoptotic protease-activating factors; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR017251; Apaf-1.
DR InterPro; IPR041452; APAF1_C.
DR InterPro; IPR037963; APAF1_CARD_dom.
DR InterPro; IPR048975; APAF1_WHD.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR001315; CARD.
DR InterPro; IPR011029; DEATH-like_dom_sf.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR22845; APOPTOTIC PROTEASE-ACTIVATING FACTOR 1; 1.
DR PANTHER; PTHR22845:SF5; APOPTOTIC PROTEASE-ACTIVATING FACTOR 1; 1.
DR Pfam; PF21296; APAF-1-like_WHD; 1.
DR Pfam; PF17908; APAF1_C; 1.
DR Pfam; PF00619; CARD; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF00400; WD40; 9.
DR PIRSF; PIRSF037646; Apop_pept_activating-1; 1.
DR PRINTS; PR00364; DISEASERSIST.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 13.
DR SUPFAM; SSF47986; DEATH domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 2.
DR PROSITE; PS50209; CARD; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 9.
DR PROSITE; PS50294; WD_REPEATS_REGION; 6.
PE 4: Predicted;
KW Apoptosis {ECO:0000256|ARBA:ARBA00022703, ECO:0000256|PIRNR:PIRNR037646};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR037646, ECO:0000256|PIRSR:PIRSR037646-
KW 50}; Cytoplasm {ECO:0000256|PIRNR:PIRNR037646};
KW Hydrolase {ECO:0000313|RefSeq:XP_004742988.1,
KW ECO:0000313|RefSeq:XP_004742989.1};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR037646,
KW ECO:0000256|PIRSR:PIRSR037646-50};
KW Protease {ECO:0000313|RefSeq:XP_004742988.1,
KW ECO:0000313|RefSeq:XP_004742989.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000000715};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT DOMAIN 1..90
FT /note="CARD"
FT /evidence="ECO:0000259|PROSITE:PS50209"
FT REPEAT 612..653
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 654..695
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 696..739
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 740..781
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 879..911
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1000..1041
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1041..1081
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1082..1116
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1124..1165
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT BINDING 155..162
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR037646-50"
FT BINDING 266
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR037646-50"
SQ SEQUENCE 1250 AA; 142069 MW; 099EA14F5CE3FD37 CRC64;
MDAKARNCLL QHREALEKDI KTSYIMDRMI NDGVLTVSEE EKVKNEPTQQ QRAALLIKMI
LKKDNYSYIS FYNALIHEGY KDLAALLQSG IPVISSSSGG KDSVGGITSY VKTVLCEGGV
PQRPVVFVTR KKLVNAIQQN LLKLNGEPGW VVIYGMAGCG KSVLAAESVR DHFFLDGCFP
GGVHWVSVGK QDKSGLLMKL QNLCTRLDQD ESFSQRLPLN IEEAKDRLRI LMLRKHPRSL
LILDDIWDPW VLKAFDNQCQ ILLTTRDKSV TDSVMGPKYV VSVESGLGKE KGLEILSLFV
NMKKADLPEQ AHSIIKECKG SPLVVSLIGA LLRDFPNRWD YYLRQLQNKQ FKRIRKSSSY
DYEALDEAMS ISVEMLREDI KDYYTDLSIL QKDVKVPTKV LCILWDMETE EVEDILQEFV
NKSLLFCDRN GKSFCYYLHD LQVDFLIEKN RNQLQNLHRK IITQFQKHYQ PHTLSPDQED
CMYWYNFLAY HMGSANMHKE LCALMFSLDW IKAKTELVGP AHLIHEFVEY RHILDEKDCA
VCENFQEFLS LNGHLLGRQP FPNIVQLGLC EPETSEVYQQ AKLQAKQEVD HGVLYLEWIN
KKNIKNLSRL VVRPHTDAVY HACFSEDGQR IASCGVDKTL QVFKAETGEK LLEIKAHEDE
VLCCAFSTDD RFIATCSVDK KVKIWNSMTG ELVRIYDEHS EQVNCCHFAN NSQYLLLATA
SSDCFLKLWD LNQKECRNTM FGHTNSVNHC RFSPDDKLLA SCSADGTLKL WDVKSANERK
SIDVKQFFLN SEEPQEDMEV IVKCCSWSAD GARIMVAAKN KIFLFDIHTN GLLAEIHTGH
HSTIQYCDFS PQNHLAVVAL SQCCVELWNM DSCLKVADCR GHLSWVHCVM FSPDGSSFLT
SSDDQTIRLW ETKKVCKNSA IVLKQEIDVV FQENEVMVLA ADNIKRLQLI NGKTGQIDYL
TEAQVSCCCL SPYLQYIAFG GEDGAIEILE LLNNRIFQSR IGHKKTVRHI QFTDDGKTLI
SSSDDSSIQV WNWQSEEYVF LQAHQETVKD FKLLKNSKLL SWSFDGTVKV WSIITGRIEK
DFVCHQDTVL SCDISPDATK FSSTSADKTA KIWSFDLLSP LHELRGHRGC VRCSVFSVDS
TLLATGDDNG EIRIWNVSNG ELLHLCAPIS VEEGAATHGG WVTDLCFSPD SKMLVSAGGY
LKWWNVVTGE SSQTFYTNGT YLKKIHVSPD FKTYVTVDNL GILYILQMLE
//
