ID M4RBJ8_9BIFI Unreviewed; 1190 AA.
AC M4RBJ8;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 53.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN Name=dnaE {ECO:0000313|EMBL:AGH40851.1};
GN ORFNames=D805_0584 {ECO:0000313|EMBL:AGH40851.1};
OS Bifidobacterium thermophilum RBL67.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Bifidobacterium.
OX NCBI_TaxID=1254439 {ECO:0000313|EMBL:AGH40851.1, ECO:0000313|Proteomes:UP000011835};
RN [1] {ECO:0000313|EMBL:AGH40851.1, ECO:0000313|Proteomes:UP000011835}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RBL67 {ECO:0000313|EMBL:AGH40851.1,
RC ECO:0000313|Proteomes:UP000011835};
RX PubMed=23640377;
RA Jans C., Lacroix C., Follador R., Stevens M.J.;
RT "Complete Genome Sequence of the Probiotic Bifidobacterium thermophilum
RT Strain RBL67.";
RL Genome Announc. 1:E00191-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP004346; AGH40851.1; -; Genomic_DNA.
DR RefSeq; WP_015450112.1; NC_020546.1.
DR AlphaFoldDB; M4RBJ8; -.
DR KEGG; btp:D805_0584; -.
DR PATRIC; fig|1254439.12.peg.586; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_0_0_11; -.
DR Proteomes; UP000011835; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000313|EMBL:AGH40851.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AGH40851.1}.
FT DOMAIN 8..75
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1190 AA; 132855 MW; 31BF981DDA66F042 CRC64;
MTDSGNFVQL HNHTHYSLLD GASKIPDLVK RVKELGMPAV GITDHGNMHG AYELWRNAVD
QGIKPIIGIE AYVTPETARQ DKTRVSWDTN WVPQPHHERN PDDVSGGGLI THLTLWAEND
EGLVNLIKAS SVANLEGRVM RYPRMDKEVL QRYHKGVICS SGCPSGIIQT RLRLGQFDEA
LRAAGEMQDI FGKDNFYIEL MDHGLEIERR VTKDLLTIAK KLNAPLLATN DSHYVHEEDR
DAQDAMLCIN SGSRLDDPDR FKFDGSGYYI KSAQEMRDLF KELPEACDNT LAIADRCNVI
FDDHEDGAFM PRFDCPEGWD ETSLFLKHVD EGLKRRYNGN PPIEVLKQAD YECGVICQMQ
FCGYFLVVAD YINWAKSHGV MVGPGRGSAA GSMVAYAMGI TELDPLKHGL IFERFLNPER
VSLPDIDVDF DPDGRMKVLD YVANKYGHDK VAQCVIYGTI KTKQALKDSA RIMGYEFSVG
ERITKALPPS KNGKDASLHD IFDPTSKRYA EAREFRELYD ADPDAKRITE EAKGIEGLIR
QTGVHACATI MGSEAITNTS PLLERTDGTI TTTFEYHTCE TLGLVKMDFL GLSNLTVIRD
TLTNIRNNGK EPIDYTKIPL DDKPTYELLA RGDTLGVFQL DSDGMRSLLK TLKPDNFNDI
SALIALYRPG PMDMDSHNNY AKRKNGLQKI TPIHPEVAEP LKEVLGETYG LIVYQEQVQS
AARILAGYSL GKADVLRRAM GKKKPEVLAK EKVPFFQGMK EHGYSMEAAQ AVWDILVPFS
GYAFNKAHSA AYGLISYWTA YLKTHYPVEF MAALLQNERT NKDKTALYLG EARRMGIKVL
PPDVNESRLE YSAVGDVVRF GLGAIRNVGD KAVEDIIAER ETARGKYVNF MDFIRRVPVT
ALNRRLVESL IKAGAFDSID PNRRALYEIH ETAIDSVIGL KRKQAEGQFD LFADDDDGGA
DMMGDASVVV PDIEEWDKKT KLNFEREMLG LYVSDHPLSG MTAILANLRE MSIAHLIDRA
KTMGDSQQVT IAGLVTNVDR RVSKKGNPWA IVTVEDMESS IQCMFFGKVY ENAAAQLAID
EVVQVRGQVE VRDETVSLRA TEMQVPSLEA DDSRPVVITL PPVALDRDHA MQLGRVLMNH
PGYCPVKLAI MGEKGDAQVL TFGDRFRVKR DTSLFAEIKI LFGPSCLPAA
//
