ID M4XKU7_9PSED Unreviewed; 246 AA.
AC M4XKU7;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 62.
DE RecName: Full=Sulfate transporter CysZ {ECO:0000256|HAMAP-Rule:MF_00468};
GN Name=cysZ {ECO:0000256|HAMAP-Rule:MF_00468};
GN ORFNames=H681_19990 {ECO:0000313|EMBL:AGI25865.1};
OS Pseudomonas sp. ATCC 13867.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1294143 {ECO:0000313|EMBL:AGI25865.1, ECO:0000313|Proteomes:UP000012082};
RN [1] {ECO:0000313|EMBL:AGI25865.1, ECO:0000313|Proteomes:UP000012082}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13867 {ECO:0000313|EMBL:AGI25865.1,
RC ECO:0000313|Proteomes:UP000012082};
RX PubMed=23723394;
RA Ainala S.K., Somasundar A., Park S.;
RT "Complete genome sequence of Pseudomonas denitrificans ATCC 13867.";
RL Genome Announc. 1:E00257-E00213(2013).
RN [2] {ECO:0007829|PDB:6D9Z}
RP X-RAY CRYSTALLOGRAPHY (3.40 ANGSTROMS).
RX PubMed=29792261; DOI=10.7554/eLife.27829;
RA Assur Sanghai Z., Liu Q., Clarke O.B., Belcher-Dufrisne M.,
RA Wiriyasermkul P., Giese M.H., Leal-Pinto E., Kloss B., Tabuso S., Love J.,
RA Punta M., Banerjee S., Rajashankar K.R., Rost B., Logothetis D., Quick M.,
RA Hendrickson W.A., Mancia F.;
RT "Structure-based analysis of CysZ-mediated cellular uptake of sulfate.";
RL Elife 7:0-0(2018).
CC -!- FUNCTION: High affinity, high specificity proton-dependent sulfate
CC transporter, which mediates sulfate uptake. Provides the sulfur source
CC for the cysteine synthesis pathway. {ECO:0000256|HAMAP-Rule:MF_00468}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_00468}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_00468}.
CC -!- SIMILARITY: Belongs to the CysZ family. {ECO:0000256|HAMAP-
CC Rule:MF_00468}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00468}.
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DR EMBL; CP004143; AGI25865.1; -; Genomic_DNA.
DR RefSeq; WP_015478717.1; NC_020829.1.
DR PDB; 6D9Z; X-ray; 3.40 A; A/B/C/D/E/F=1-246.
DR PDBsum; 6D9Z; -.
DR AlphaFoldDB; M4XKU7; -.
DR SMR; M4XKU7; -.
DR STRING; 1294143.H681_19990; -.
DR TCDB; 2.A.121.1.4; the sulfate transporter (cysz) family.
DR KEGG; pdr:H681_19990; -.
DR PATRIC; fig|1294143.3.peg.4051; -.
DR eggNOG; COG2981; Bacteria.
DR HOGENOM; CLU_070331_1_0_6; -.
DR OrthoDB; 5292355at2; -.
DR Proteomes; UP000012082; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015116; F:sulfate transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0000103; P:sulfate assimilation; IEA:InterPro.
DR HAMAP; MF_00468; CysZ; 1.
DR InterPro; IPR022985; Sulfate_CysZ.
DR PANTHER; PTHR37468; SULFATE TRANSPORTER CYSZ; 1.
DR PANTHER; PTHR37468:SF1; SULFATE TRANSPORTER CYSZ; 1.
DR Pfam; PF07264; EI24; 1.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:6D9Z};
KW Amino-acid biosynthesis {ECO:0000256|HAMAP-Rule:MF_00468};
KW Cell inner membrane {ECO:0000256|ARBA:ARBA00022519, ECO:0000256|HAMAP-
KW Rule:MF_00468};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, ECO:0000256|HAMAP-
KW Rule:MF_00468}; Cysteine biosynthesis {ECO:0000256|HAMAP-Rule:MF_00468};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_00468};
KW Sulfate transport {ECO:0000256|ARBA:ARBA00023032, ECO:0000256|HAMAP-
KW Rule:MF_00468};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_00468};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_00468};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|HAMAP-Rule:MF_00468}.
FT TRANSMEM 24..45
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00468"
FT TRANSMEM 65..94
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00468"
FT TRANSMEM 201..234
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00468"
SQ SEQUENCE 246 AA; 27715 MW; BB8BDA431EDAE66A CRC64;
MSTLSGPQYL GEGLKLMMRP GLRLFVLLPL SINLILFIGL IGFAINQFSH WVDWLMPSLP
EWLSFLQFIL WPLFVTLVLL IVFFTFTLIA NLIAAPFNGF LAEKVEVVVR GTDDFPAFSW
AELMAMVPRT IGRELRKLGY FLPRAIALFI LSLIPGLNLI AAPLWLLFGV WMMAVQYIDY
PADNHKLGWN EMLAWLRSKR WACMGFGGIT YLVLLIPLVN LVAMPAAVAG AVLFWVREGG
DQALVK
//
