ID M4ZTD2_9FLAO Unreviewed; 614 AA.
AC M4ZTD2;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 60.
DE RecName: Full=DNA primase {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811};
DE EC=2.7.7.101 {ECO:0000256|HAMAP-Rule:MF_00974};
GN Name=dnaG {ECO:0000256|HAMAP-Rule:MF_00974,
GN ECO:0000313|EMBL:BAM99588.1};
GN ORFNames=BPAA_296 {ECO:0000313|EMBL:BAM99588.1};
OS Blattabacterium cuenoti BPAA.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Blattabacteriaceae; Blattabacterium.
OX NCBI_TaxID=1229512 {ECO:0000313|EMBL:BAM99588.1, ECO:0000313|Proteomes:UP000011815};
RN [1] {ECO:0000313|EMBL:BAM99588.1, ECO:0000313|Proteomes:UP000011815}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BPAA {ECO:0000313|EMBL:BAM99588.1,
RC ECO:0000313|Proteomes:UP000011815};
RX PubMed=23515978; DOI=10.1098/rsbl.2012.1153;
RA Tokuda G., Elbourne L.D.H., Kinjo Y., Saitoh S., Sabree Z., Hojo M.,
RA Yamada A., Hayashi Y., Shigenobu S., Bandi C., Paulsen I.T., Watanabe H.,
RA Lo N.;
RT "Maintenance of essential amino acid synthesis pathways in the
RT Blattabacterium cuenoti symbiont of a wood-feeding cockroach.";
RL Biol. Lett. 9:20121153-20121153(2013).
CC -!- FUNCTION: RNA polymerase that catalyzes the synthesis of short RNA
CC molecules used as primers for DNA polymerase during DNA replication.
CC {ECO:0000256|HAMAP-Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.;
CC EC=2.7.7.101; Evidence={ECO:0000256|HAMAP-Rule:MF_00974};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRNR:PIRNR002811};
CC Note=Binds 1 zinc ion per monomer. {ECO:0000256|PIRNR:PIRNR002811};
CC -!- SUBUNIT: Monomer. Interacts with DnaB. {ECO:0000256|HAMAP-
CC Rule:MF_00974}.
CC -!- SIMILARITY: Belongs to the DnaG primase family. {ECO:0000256|HAMAP-
CC Rule:MF_00974, ECO:0000256|PIRNR:PIRNR002811}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00974}.
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DR EMBL; AP012548; BAM99588.1; -; Genomic_DNA.
DR RefSeq; WP_015429907.1; NC_020510.1.
DR AlphaFoldDB; M4ZTD2; -.
DR STRING; 1229512.BPAA_296; -.
DR KEGG; blp:BPAA_296; -.
DR PATRIC; fig|1229512.3.peg.292; -.
DR eggNOG; COG0358; Bacteria.
DR HOGENOM; CLU_013501_3_0_10; -.
DR Proteomes; UP000011815; Chromosome.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:1990077; C:primosome complex; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003896; F:DNA primase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd03364; TOPRIM_DnaG_primases; 1.
DR Gene3D; 3.40.1360.10; -; 1.
DR Gene3D; 3.90.980.10; DNA primase, catalytic core, N-terminal domain; 1.
DR Gene3D; 3.90.580.10; Zinc finger, CHC2-type domain; 1.
DR HAMAP; MF_00974; DNA_primase_DnaG; 1.
DR InterPro; IPR037068; DNA_primase_core_N_sf.
DR InterPro; IPR006295; DNA_primase_DnaG.
DR InterPro; IPR036977; DNA_primase_Znf_CHC2.
DR InterPro; IPR030846; DnaG_bac.
DR InterPro; IPR013264; DNAG_N.
DR InterPro; IPR034151; TOPRIM_DnaG_bac.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR002694; Znf_CHC2.
DR NCBIfam; TIGR01391; dnaG; 1.
DR PANTHER; PTHR30313; DNA PRIMASE; 1.
DR PANTHER; PTHR30313:SF2; DNA PRIMASE; 1.
DR Pfam; PF08275; DNAG_N; 1.
DR Pfam; PF13155; Toprim_2; 1.
DR Pfam; PF01807; zf-CHC2; 1.
DR PIRSF; PIRSF002811; DnaG; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SMART; SM00400; ZnF_CHCC; 1.
DR SUPFAM; SSF56731; DNA primase core; 1.
DR SUPFAM; SSF57783; Zinc beta-ribbon; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|HAMAP-Rule:MF_00974};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR002811};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|HAMAP-Rule:MF_00974};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00974};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRNR:PIRNR002811};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 260..341
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 614 AA; 72128 MW; E5F41600555990B4 CRC64;
MISKETIKKI LSASCIEDVI GDFVKLKKSG FNYRGLSPFS NEKTPSLIVS PTKKIWKDFS
SGKGGNIITF LMEHEHFTYE ESLRFLAKKY EIKMDHIDNN NHQEKEEYEK SYLIQDYAKR
FFIYQLHFTK EGQNNGLNYF IQKRGFDINI IHKFELGYAP ISWNLLTINA LKKGFKIRDI
KESGVTVFRK SNHFFDCFRK RVMFPIHSLS GRVIGFGGRS LDSRSFTTTK YINSSESKIF
QKSKILYGLF QAKKNILKEN FCYLVEGYTD VISLHQSGIK NVVSSSGISL TVAQILLIKK
FTKNIVLFYD GDRSGIKASL RGINMMLEQE MNLRILFISN GEDPDLISKK YSFSQFRDFV
AKNSYNFVSF KQKIYEKIHK DDPIKKSFLV KNILNSISKI SNVIQKELYL QEASKVLNIR
QKVLISELKK INKKNVQKPS TVQVKGNSRF FLKKNTLLLL EEKLIQLILN HGNQIIKKKE
HNTTILEEVL HVFKCWNLRF SLNKNQEIFD QICLQKKRID LSQFLDKKNP KFYSLSKWDR
KGIQVSYQKE RINQYISDIL LRYKSLCISK LIQKEIIHYQ NDLKKNKDRK AFLKKIMHLT
DIKNELHKKL HRYV
//
