ID M5DRM0_9GAMM Unreviewed; 868 AA.
AC M5DRM0;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN ORFNames=TOL_1637 {ECO:0000313|EMBL:CCU72061.1};
OS Thalassolituus oleivorans MIL-1.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Oceanospirillaceae; Thalassolituus.
OX NCBI_TaxID=1298593 {ECO:0000313|EMBL:CCU72061.1, ECO:0000313|Proteomes:UP000011866};
RN [1] {ECO:0000313|EMBL:CCU72061.1, ECO:0000313|Proteomes:UP000011866}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MIL-1 {ECO:0000313|EMBL:CCU72061.1,
RC ECO:0000313|Proteomes:UP000011866};
RX PubMed=23599290; DOI=10.1128/genomeA.00141-13;
RA Golyshin P.N., Werner J., Chernikova T.N., Tran H., Ferrer M.,
RA Yakimov M.M., Teeling H., Golyshina O.V.;
RT "Genome Sequence of Thalassolituus oleivorans MIL-1 (DSM 14913T).";
RL Genome Announc. 1:1-3(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; HF680312; CCU72061.1; -; Genomic_DNA.
DR AlphaFoldDB; M5DRM0; -.
DR STRING; 187493.CN03_10005; -.
DR KEGG; tol:TOL_1637; -.
DR PATRIC; fig|1298593.3.peg.1568; -.
DR eggNOG; COG1048; Bacteria.
DR HOGENOM; CLU_013476_2_1_6; -.
DR Proteomes; UP000011866; Chromosome.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000011866}.
FT DOMAIN 69..541
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 663..795
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 868 AA; 94804 MW; A45F51AFA194A508 CRC64;
MINMNTEFRK PLPNSVLEYY DTRAAVDAIK PGAYAKLPYT SRILAEQLVR RCDPAVLTES
LVQLIDRKRD LDFPWYPARV VTHDILGQTA LVDLAGLRDA IAEQGGDPSK VNPVVQTQLI
VDHSLAVECG GFDPDAFQKN RDIEERRNED RFHFIEWTKT AFDNVDVIPA GNGIMHQINL
EKMSPVVQAR DGVAYPDTCV GTDSHTPHVD ALGVISVGVG GLEAETVMLG HPSMMRLPDI
VGVELTGKRQ SGITATDIVL ALTEFLRKER VVGAYLEFYG EGASSLTIGD RATISNMTPE
YGATAAMFSI DEQTIEYLTL TGREPEQVAL VEAFAKETGL WADDLKTAEY ERVLSFDLSS
VGRNIAGPSN PHARVSTADL AAKGISGNLA SAQAEEAQGL MPDGAVIIAA ITSCTNTSNP
RNVVAAGLLA KKANELGLVR KPWVKSSFAP GSKVAELYLK DSGLLPELEK LGFGIVAFAC
TTCNGMSGAL DPVIQQEIID RDLYATAVLS GNRNFDGRIH PYAKQAFLAS PPLVVAYAIA
GTIRFDIEQD VLAVVDGKEI RLKDIWPSDE EIDAIVKASV KPEQFREIYI PMFDLGEFKK
ADSPLYDWRP MSTYIRRPPY WEGALAGERS LKGMRPLAVL PDNITTDHLS PSNAILMNSA
AGEYLHKMGV PEEDFNSYAT HRGDHLTAQR ATFANPKLLN EMVLDDNGNV QQGSLARIEP
EGKVTRMWEA IETYMERKQP LIIVAGADYG QGSSRDWAAK GVRLAGVEAI AAEGFERIHR
TNLIGMGVLP LEFKPGTTRK TLGLDGTETY DVIGERSARC DLTLVITRKN GEVVEVPMTC
RLDTADEVIT YDAGGVLQKF AQDFLKNA
//
