ID M5E162_9FIRM Unreviewed; 276 AA.
AC M5E162;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE SubName: Full=2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases {ECO:0000313|EMBL:CCU79335.1};
GN ORFNames=HSACCH_01247 {ECO:0000313|EMBL:CCU79335.1};
OS Halanaerobium saccharolyticum subsp. saccharolyticum DSM 6643.
OC Bacteria; Bacillota; Clostridia; Halanaerobiales; Halanaerobiaceae;
OC Halanaerobium.
OX NCBI_TaxID=1293054 {ECO:0000313|EMBL:CCU79335.1, ECO:0000313|Proteomes:UP000012063};
RN [1] {ECO:0000313|Proteomes:UP000012063}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6643 {ECO:0000313|Proteomes:UP000012063};
RX PubMed=23640197; DOI=10.1128/genomea.00187-13;
RA Kivisto A., Larjo A., Ciranna A., Santala V., Roos C., Karp M.;
RT "Genome Sequence of Halanaerobium saccharolyticum subsp. saccharolyticum
RT Strain DSM 6643T, a Halophilic Hydrogen-Producing Bacterium.";
RL Genome Announc. 1:217-225(2013).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|PIRSR:PIRSR006816-1};
CC Note=Binds 1 FAD per subunit. {ECO:0000256|PIRSR:PIRSR006816-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR006816-2};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR006816-2};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCU79335.1}.
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DR EMBL; CAUI01000015; CCU79335.1; -; Genomic_DNA.
DR RefSeq; WP_005488672.1; NZ_CAUI01000015.1.
DR AlphaFoldDB; M5E162; -.
DR STRING; 1293054.HSACCH_01247; -.
DR eggNOG; COG0543; Bacteria.
DR InParanoid; M5E162; -.
DR OrthoDB; 9778346at2; -.
DR Proteomes; UP000012063; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0006221; P:pyrimidine nucleotide biosynthetic process; IEA:InterPro.
DR CDD; cd06219; DHOD_e_trans_like1; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR012165; Cyt_c3_hydrogenase_gsu.
DR InterPro; IPR019480; Dihydroorotate_DH_Fe-S-bd.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR43513; DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT; 1.
DR PANTHER; PTHR43513:SF3; DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED; 1.
DR Pfam; PF10418; DHODB_Fe-S_bind; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR PIRSF; PIRSF006816; Cyc3_hyd_g; 1.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS51384; FAD_FR; 1.
PE 4: Predicted;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR006816-2};
KW FAD {ECO:0000256|PIRSR:PIRSR006816-1};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR006816-1};
KW Iron {ECO:0000256|PIRSR:PIRSR006816-2};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR006816-2};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR006816-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000012063}.
FT DOMAIN 1..95
FT /note="FAD-binding FR-type"
FT /evidence="ECO:0000259|PROSITE:PS51384"
FT BINDING 62..64
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR006816-1"
FT BINDING 222
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR006816-2"
FT BINDING 225
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR006816-2"
FT BINDING 237
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR006816-2"
SQ SEQUENCE 276 AA; 30762 MW; 394284A9D2C1E471 CRC64;
MYEILEKEVL APTIKKLKVK APLIAKKTEP GNFIILRVDE KGERIPLTVA DYERETGIIS
IIFQEVGYST KLLGKMDKGD YIQDIVGPLG HHIEMEGYNK VVLLGGGSGT ALLYPKVRGF
YEQGAEVISI TGARTKNLII LEDELNEYSD RVYIATDDGS YGHHGFVTEI LKDLLEEEDD
IDLVVAIGPV PMMKAVADMT EEYGIETIVS LNTIMVDGTG MCGACRVTVG GERKFTCVDG
PAFDAHQVDF EELMNRLNFY QNEEDLVHYK EVEEDN
//
