ID M5FWI3_DACPD Unreviewed; 2139 AA.
AC M5FWI3;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=separase {ECO:0000256|ARBA:ARBA00012489};
DE EC=3.4.22.49 {ECO:0000256|ARBA:ARBA00012489};
GN ORFNames=DACRYDRAFT_116022 {ECO:0000313|EMBL:EJU02296.1};
OS Dacryopinax primogenitus (strain DJM 731) (Brown rot fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Dacrymycetes;
OC Dacrymycetales; Dacrymycetaceae; Dacryopinax.
OX NCBI_TaxID=1858805 {ECO:0000313|EMBL:EJU02296.1, ECO:0000313|Proteomes:UP000030653};
RN [1] {ECO:0000313|EMBL:EJU02296.1, ECO:0000313|Proteomes:UP000030653}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DJM-731 SS1 {ECO:0000313|EMBL:EJU02296.1,
RC ECO:0000313|Proteomes:UP000030653};
RX PubMed=22745431; DOI=10.1126/science.1221748;
RA Floudas D., Binder M., Riley R., Barry K., Blanchette R.A., Henrissat B.,
RA Martinez A.T., Otillar R., Spatafora J.W., Yadav J.S., Aerts A., Benoit I.,
RA Boyd A., Carlson A., Copeland A., Coutinho P.M., de Vries R.P.,
RA Ferreira P., Findley K., Foster B., Gaskell J., Glotzer D., Gorecki P.,
RA Heitman J., Hesse C., Hori C., Igarashi K., Jurgens J.A., Kallen N.,
RA Kersten P., Kohler A., Kuees U., Kumar T.K.A., Kuo A., LaButti K.,
RA Larrondo L.F., Lindquist E., Ling A., Lombard V., Lucas S., Lundell T.,
RA Martin R., McLaughlin D.J., Morgenstern I., Morin E., Murat C., Nagy L.G.,
RA Nolan M., Ohm R.A., Patyshakuliyeva A., Rokas A., Ruiz-Duenas F.J.,
RA Sabat G., Salamov A., Samejima M., Schmutz J., Slot J.C., St John F.,
RA Stenlid J., Sun H., Sun S., Syed K., Tsang A., Wiebenga A., Young D.,
RA Pisabarro A., Eastwood D.C., Martin F., Cullen D., Grigoriev I.V.,
RA Hibbett D.S.;
RT "The Paleozoic origin of enzymatic lignin decomposition reconstructed from
RT 31 fungal genomes.";
RL Science 336:1715-1719(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=All bonds known to be hydrolyzed by this endopeptidase have
CC arginine in P1 and an acidic residue in P4. P6 is often occupied by
CC an acidic residue or by a hydroxy-amino-acid residue, the
CC phosphorylation of which enhances cleavage.; EC=3.4.22.49;
CC Evidence={ECO:0000256|ARBA:ARBA00000451};
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DR EMBL; JH795862; EJU02296.1; -; Genomic_DNA.
DR STRING; 1858805.M5FWI3; -.
DR HOGENOM; CLU_000777_0_0_1; -.
DR OMA; VAFCEYW; -.
DR OrthoDB; 5479815at2759; -.
DR Proteomes; UP000030653; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:InterPro.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0098813; P:nuclear chromosome segregation; IEA:UniProt.
DR GO; GO:0000280; P:nuclear division; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:InterPro.
DR InterPro; IPR005314; Peptidase_C50.
DR InterPro; IPR030397; SEPARIN_core_dom.
DR PANTHER; PTHR12792; EXTRA SPINDLE POLES 1-RELATED; 1.
DR PANTHER; PTHR12792:SF0; SEPARIN; 1.
DR Pfam; PF03568; Peptidase_C50; 1.
DR PROSITE; PS51700; SEPARIN; 1.
PE 4: Predicted;
KW Chromosome partition {ECO:0000256|ARBA:ARBA00022829};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000030653}.
FT DOMAIN 1952..2049
FT /note="Peptidase C50"
FT /evidence="ECO:0000259|PROSITE:PS51700"
FT REGION 1..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 121..147
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 956..999
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1209..1238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..39
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2139 AA; 235848 MW; 0D821BF14A375FFC CRC64;
MPPAKTTRSA NTTQATSTRN RTTSGKTVKS TNANSQGSDD VDALAGRMTG TLRLNATSSV
GRKPTRTTST TVRTENPHSK STAENVPVTP KLELSPTERG KHAMRTVNST MQSLVEAIQK
GWKAPHAQSK PTAPIRSRAL NKQEDSGEGV CTLESITSTA AKCRSALSSM RGLKDQVDIK
PVDIERAAVN VAGKLLGLEM WTLVLEFLTD SRPSVLDLYE HLPLSARITK TSTPAVGKSR
TGQRVPSSRS VVKESTSFGQ HCELLLFPTP SDPDNIDVAA LNVIASSQLY ALTSLVHLVN
PASIRELSQT LAAPGNMLDF VPVLQTRLGS KFYSSLALST FRAIATRLSS SEAHSPDVLF
SVRLWALRCF IQADGINGND FWDQTMRWAA SYLKTIPSGS KDQEKAASAQ IMTVFSELVD
RAGARPDGRD FANGASFGKM CDWWISLARR SGNLEILTTV NNILASTSTT ATSIKSSSNL
DTRVAANESQ GMDDAGMLLS KIYARVSHVE ALVSKLAKDG DDKGVEDHLD AILSDIRHLR
RLYTSGVDGV HRLQRETERL RHSCAKLLEK TLEASLSGGR LRPACIAVLE SLIDVAESTS
TPADGDSVPN KPSAEFQKDM MSGALDSLLT LARVVLSPTQ HLTYSLALGY LERAHKLTTS
IPSGETVQYL RCLGAAHQNA AAVLYKHNKA EFAIRFAEQA YLISVQMFEA SDGANNEGSA
LREQLGRRAE ILASCYLKQG DRKLAYRSYC DALSWISFNT ISALQSTLAK ESVFASFAAQ
PTVSALIERV TRLAVSDLLL PPKEASLRHQ LEGRAVPTEV IGGLLEYQIL TMDNMLHKPE
CLPAISNILD ELLNLYAPDI FPIRRSRVLL RNLEHLYHIG SNSSALIASD ILEGFVGENK
ANDAGLAGYI PLYQAFTHVW LAIQSHGNAD SPSMPAVVQH TDEAVRIFRQ VFIPGSVPKP
RSASPKSRSL GKGPSKPRPA QTPKTPARGR RAVSTKTPKV TTSLTLSNDR ATITSAALDN
VERLYKHLEL LSDLLKFLVL SFQRIDLLEI MRHFNQHRIE RSLSCDEMLR TTTELAYENA
RLGKTGRASH LYAQAMNCAL HDDCTTPLSD SARILYLLRY ASSLVIHGNL EKSSSLYQEA
LETAAAMETD GPNTTVGRIR TRTLVLEQAA IASSVYALIK DEQDDMPSCL NALEQSLRLW
NRAMDNVMRL NPSHSPPPPS DLDNPFVDDD TTTKPKQSAA KIDTRKCVAL DGLQWRVAAG
MFQALLFLAE YHCRAGAPRA ALLYFGKAVE MADGLAAPLP HGTALALQEE LKLMCEDPVG
EGFSRSDELL GQLPTAGLIE MRRIQGDQCV KEENKDLGRQ IYASADTILK DLEKTYTLAM
TTQKTPLKIS RKRVEKPVAD GELDVLLPTL QAALLRQQVW LFKEPSEAPE FEELLTRLLS
LPPTRNLKAE ENSLLGRVHM HDACNQFRND LFLSSLTESI IAVPMGMISQ QCLTPSASSI
QVLDTLHKAE QYFADALELM LCKGRISETR SVSVNLALIH AFQTMLGKTS SNGPRLAASL
LEMCAGTSLH RQMLESTDLK LQPSKHSDDL TWPSTAPKHV PAKQNQVLLG STISDEDICS
ADDDDEEDLD RSYWQATRDR YIALLSDVGL QGPAQVDVLP SNWTVISINV TEDQNTILIA
RHRSRQEPLI LCLPLDRQGK RDGEDDQFTF QSAIDELDNI IENSNKGILA AKEVAGKDAT
ASWWSERGEL DRRLGELLET IEFCWLGAFK TVLCEPPRAT KESLKSLRNR LEKIFQGAIK
ALDKNSPASR TRLHDNILDC FASLSTKCRD EELEDMIYFV LDLYHFHGIP VAVAEIDVDM
LVVDIRSALE EFAANPPVLC SSQADPHLFL ILDKNVQGIP WESIPILRGR SVSRIPSISF
LVDRIELSRH RRGPETEPTA SPRKIDRVVV DPRRAFYMLN VSRDLTRTEQ QFSPWLKEMD
QIGWRGIIGR PPMAGEMLRA LESSELVLYF GHGGGEQYVR SQQIRRLSRC ASVMLWGCSS
GALRSMGEFD RFGTPHNYML AGCPNVVATL WDVTDRDIDR FSDSVFKKMH LDRAHVETWS
ASQPSMTSIV AAVAQSRDVC KLKYLNGAAP VVYGIPYYL
//
