ID M5X751_PRUPE Unreviewed; 1029 AA.
AC M5X751;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 64.
DE RecName: Full=Translation initiation factor IF-2, chloroplastic {ECO:0000256|ARBA:ARBA00044105};
GN ORFNames=PRUPE_4G144300 {ECO:0000313|EMBL:ONI12102.1};
OS Prunus persica (Peach) (Amygdalus persica).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX NCBI_TaxID=3760 {ECO:0000313|EMBL:ONI12102.1, ECO:0000313|Proteomes:UP000006882};
RN [1] {ECO:0000313|EMBL:ONI12102.1, ECO:0000313|Proteomes:UP000006882}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nemared {ECO:0000313|Proteomes:UP000006882};
RX PubMed=23525075; DOI=10.1038/ng.2586;
RA Verde I., Abbott A.G., Scalabrin S., Jung S., Shu S., Marroni F.,
RA Zhebentyayeva T., Dettori M.T., Grimwood J., Cattonaro F., Zuccolo A.,
RA Rossini L., Jenkins J., Vendramin E., Meisel L.A., Decroocq V.,
RA Sosinski B., Prochnik S., Mitros T., Policriti A., Cipriani G., Dondini L.,
RA Ficklin S., Goodstein D.M., Xuan P., Del Fabbro C., Aramini V., Copetti D.,
RA Gonzalez S., Horner D.S., Falchi R., Lucas S., Mica E., Maldonado J.,
RA Lazzari B., Bielenberg D., Pirona R., Miculan M., Barakat A., Testolin R.,
RA Stella A., Tartarini S., Tonutti P., Arus P., Orellana A., Wells C.,
RA Main D., Vizzotto G., Silva H., Salamini F., Schmutz J., Morgante M.,
RA Rokhsar D.S.;
RT "The high-quality draft genome of peach (Prunus persica) identifies unique
RT patterns of genetic diversity, domestication and genome evolution.";
RL Nat. Genet. 45:487-494(2013).
CC -!- FUNCTION: One of the essential components for the initiation of protein
CC synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis
CC and promotes its binding to the 30S ribosomal subunits. Also involved
CC in the hydrolysis of GTP during the formation of the 70S ribosomal
CC complex. {ECO:0000256|ARBA:ARBA00025162}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733}.
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DR EMBL; CM007654; ONI12102.1; -; Genomic_DNA.
DR RefSeq; XP_007213706.1; XM_007213644.1.
DR AlphaFoldDB; M5X751; -.
DR STRING; 3760.M5X751; -.
DR EnsemblPlants; ONI12102; ONI12102; PRUPE_4G144300.
DR GeneID; 18778617; -.
DR Gramene; ONI12102; ONI12102; PRUPE_4G144300.
DR KEGG; pper:18778617; -.
DR eggNOG; KOG1145; Eukaryota.
DR HOGENOM; CLU_006301_7_1_1; -.
DR OMA; CGIGVED; -.
DR OrthoDB; 169393at2759; -.
DR Proteomes; UP000006882; Chromosome g4.
DR GO; GO:0009507; C:chloroplast; IEA:EnsemblPlants.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003729; F:mRNA binding; IEA:EnsemblPlants.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR CDD; cd01887; IF2_eIF5B; 1.
DR CDD; cd03702; IF2_mtIF2_II; 1.
DR CDD; cd03692; mtIF2_IVc; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR HAMAP; MF_00100_B; IF_2_B; 1.
DR InterPro; IPR044145; IF2_II.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR000178; TF_IF2_bacterial-like.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00487; IF-2; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF5; TR-TYPE G DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 2.
DR PROSITE; PS51722; G_TR_2; 1.
DR PROSITE; PS01176; IF2; 1.
PE 3: Inferred from homology;
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000006882}.
FT DOMAIN 500..673
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 1029 AA; 109623 MW; 26F6F08919FD824D CRC64;
MLILVGSMQG TMASVASLVS LGSVTLLGSS ERSRSLVRKV SLSKASLKGS RRWHCVRLSV
CKCSVTTTDF VAKQGNEVSL DSNNYRGSTD VSNANADFVL KPSPKPVLKS SGGSNNEPLV
GIDAADWDPS RISGDSDEED GDEERNKVIE SLGEVLEKAE KLETSRAGEL GTKKDSSSVN
KPAPSNASTN LRNAKPVNSE TTSKSKTLKS VWRKGDTVAN VQKVVKESPK LNNTIPEEEL
KTGGGLKADS QPHASLRPPQ PPLRPQPKLQ AKPSAAPPPM VKKPVVLKDV GAAPKSSGID
ETDSSTQTKE RKPILIDKFA SKKPAVDSVI SQAVLAPSKP GKGPPPGRFK DGYRKKNDPG
GRRRKVDDEI PDEEASELNV SIPGAARKGR KWSKASRKAA RLQAAKEAAP VKVEILEVGE
DGMLIDDLAY YLAINESQIL GSLYAKGIKP DGVQTLDKDM VKMICKEHDV EVIDADPVKV
EEMAKKKEIL DEDDLDKLED RPPVLTIMGH VDHGKTTLLD YIRKSKVAAS EAGGITQGIG
AYKVLVPIDG KVQSCVFLDT PGHEAFGAMR ARGARVTDIA IIVVAADDGI RPQTKEAIAH
AKAAGVPIVI AINKIDKDGA NPDRVMQELS SIGLMPEDWG GDVPMVQISA LKGKNIDELL
ETVMLVAELQ DLKANPHRSA KGTVIEAGLH KSKGPLVTLI VQNGTLRRGD IIVCGGAFGK
VRALFDDGGN RVDEAGPSIP VQVLGLNNVP VAGDEFDVVG SLDVAREKAE SRAESLRSER
ISAKAGDGRV TLSSLASAVS SGKLSGLDLH QLNIILKVDL QGSIEAVRQA LQVLPQDNVT
LKFLLEATGD VSTSDVDLAA ASKAIVFGFN VKVPGSVKSY GENKGVEIRL YRVIYELIDD
VRNAMEGLLE PVEEQVTIGS AEVRAVFSSG SGRVAGCMIN EGKVVKGCGV QVIRRGKVVH
VGLLDSLKRV KEIVKEVNAG LECGIGVEDY DDWEEGDILE AFNTVQKKRT LEEASASMAA
AVEGAGIQL
//
