ID   M7CM60_CHEMY            Unreviewed;       690 AA.
AC   M7CM60;
DT   29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2013, sequence version 1.
DT   27-MAR-2024, entry version 38.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   ORFNames=UY3_00442 {ECO:0000313|EMBL:EMP42282.1};
OS   Chelonia mydas (Green sea-turtle) (Chelonia agassizi).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Testudinata; Testudines; Cryptodira; Durocryptodira;
OC   Americhelydia; Chelonioidea; Cheloniidae; Chelonia.
OX   NCBI_TaxID=8469 {ECO:0000313|EMBL:EMP42282.1, ECO:0000313|Proteomes:UP000031443};
RN   [1] {ECO:0000313|Proteomes:UP000031443}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23624526; DOI=10.1038/ng.2615;
RA   Wang Z., Pascual-Anaya J., Zadissa A., Li W., Niimura Y., Huang Z., Li C.,
RA   White S., Xiong Z., Fang D., Wang B., Ming Y., Chen Y., Zheng Y.,
RA   Kuraku S., Pignatelli M., Herrero J., Beal K., Nozawa M., Li Q., Wang J.,
RA   Zhang H., Yu L., Shigenobu S., Wang J., Liu J., Flicek P., Searle S.,
RA   Wang J., Kuratani S., Yin Y., Aken B., Zhang G., Irie N.;
RT   "The draft genomes of soft-shell turtle and green sea turtle yield insights
RT   into the development and evolution of the turtle-specific body plan.";
RL   Nat. Genet. 45:701-706(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC       {ECO:0000256|ARBA:ARBA00004906}.
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DR   EMBL; KB476411; EMP42282.1; -; Genomic_DNA.
DR   AlphaFoldDB; M7CM60; -.
DR   STRING; 8469.M7CM60; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000031443; Unassembled WGS sequence.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR   CDD; cd00078; HECTc; 1.
DR   CDD; cd00201; WW; 1.
DR   Gene3D; 2.20.70.10; -; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   InterPro; IPR001202; WW_dom.
DR   InterPro; IPR036020; WW_dom_sf.
DR   PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR   PANTHER; PTHR11254:SF299; NEDD4-LIKE E3 UBIQUITIN-PROTEIN LIGASE WWP1; 1.
DR   Pfam; PF00632; HECT; 1.
DR   Pfam; PF00397; WW; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SMART; SM00456; WW; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   SUPFAM; SSF51045; WW domain; 1.
DR   PROSITE; PS50237; HECT; 1.
DR   PROSITE; PS01159; WW_DOMAIN_1; 1.
DR   PROSITE; PS50020; WW_DOMAIN_2; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000031443};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   DOMAIN          72..105
FT                   /note="WW"
FT                   /evidence="ECO:0000259|PROSITE:PS50020"
FT   DOMAIN          139..438
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   ACT_SITE        406
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   690 AA;  78518 MW;  C56FD62770C019BE CRC64;
     MCSTHPTTSK LNHMFFGCFS SFVLPTGLHM DQTTRKRTVL NTYFKRLCIA RVGAVGADLV
     QKQVTISLQN EDPLPEGWEI RYTREGVRYF VDHNTRTTTF NDPRTGKSSV NKGPQIAYER
     SFRWKLAHFR YLCQIMALKP YDLRRRLYVI FRGEEGLDYG GLAREWFFLL SHEVLNPMYC
     LFEYAGKSNY CLQINPASTI NPDHLSYFCF IGRFIAMALF HGKFIDTGFS LPFYKRMLSK
     KLTIKDLESI DTEFYNSLIW IRDNNIEECN LEMYFCVDME LLGKVTSHEL KSGGSNILVT
     EDNKEEYIGL MAEWRFSRGV REQTKAFLDG FNEVVPLQWL QYFDEKELEF VKDTDNEVRM
     RLLQFVTGTC RLPLGGFAEL MGSNGPQKFC IEKVGKETWL PRSHTCFNRL DLPPYKSYEQ
     LKEKLLFAIE ETEGFDLDCD DDHLWERYQD RLREGDRCSD RWREQCREPE WYRCPEPCRE
     PDRCCEYDQW CKYDRRHKRK RHWDCEQSRD CERCQDWIQE QCRVCMCGDG RCIRVGLPLD
     GTVRVGGGAA VCDDAGSVRV TRSHAAANVS GVDGSWDSTT VQVGEPVRTG LNGSSLGAGV
     NGADIGACTL GCAGAGRDSE AGIGVPATVE VGQSSPACTV SSGPEIENGT RSLVLAVVPE
     SGGAAGLYQS PTAVQYLMLL RGAVHQSSQC
//