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Database: UniProt
Entry: M7Y700_TRIUA
LinkDB: M7Y700_TRIUA
Original site: M7Y700_TRIUA 
ID   M7Y700_TRIUA            Unreviewed;       822 AA.
AC   M7Y700;
DT   29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   29-MAY-2013, sequence version 1.
DT   31-JUL-2019, entry version 49.
DE   RecName: Full=Urease {ECO:0000256|PIRNR:PIRNR001222};
DE            EC=3.5.1.5 {ECO:0000256|PIRNR:PIRNR001222};
DE   AltName: Full=Urea amidohydrolase {ECO:0000256|PIRNR:PIRNR001222};
GN   ORFNames=TRIUR3_27202 {ECO:0000313|EMBL:EMS45668.1};
OS   Triticum urartu (Red wild einkorn) (Crithodium urartu).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4572 {ECO:0000313|EMBL:EMS45668.1};
RN   [1] {ECO:0000313|EMBL:EMS45668.1, ECO:0000313|EnsemblPlants:TRIUR3_27202-P1, ECO:0000313|Proteomes:UP000015106}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=cv. G1812 {ECO:0000313|EnsemblPlants:TRIUR3_27202-P1};
RX   PubMed=23535596; DOI=10.1038/nature11997;
RA   Ling H.Q., Zhao S., Liu D., Wang J., Sun H., Zhang C., Fan H., Li D.,
RA   Dong L., Tao Y., Gao C., Wu H., Li Y., Cui Y., Guo X., Zheng S.,
RA   Wang B., Yu K., Liang Q., Yang W., Lou X., Chen J., Feng M., Jian J.,
RA   Zhang X., Luo G., Jiang Y., Liu J., Wang Z., Sha Y., Zhang B., Wu H.,
RA   Tang D., Shen Q., Xue P., Zou S., Wang X., Liu X., Wang F., Yang Y.,
RA   An X., Dong Z., Zhang K., Zhang X., Luo M.C., Dvorak J., Tong Y.,
RA   Wang J., Yang H., Li Z., Wang D., Zhang A., Wang J.;
RT   "Draft genome of the wheat A-genome progenitor Triticum urartu.";
RL   Nature 496:87-90(2013).
RN   [2] {ECO:0000313|EnsemblPlants:TRIUR3_27202-P1}
RP   IDENTIFICATION.
RG   EnsemblPlants;
RL   Submitted (JUN-2015) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+);
CC         Xref=Rhea:RHEA:20557, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16199, ChEBI:CHEBI:16526, ChEBI:CHEBI:28938;
CC         EC=3.5.1.5; Evidence={ECO:0000256|PIRNR:PIRNR001222};
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516;
CC         Evidence={ECO:0000256|PIRNR:PIRNR001222,
CC         ECO:0000256|PIRSR:PIRSR001222-51};
CC       Note=Binds 2 nickel ions per subunit.
CC       {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-51};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3)
CC       from urea (urease route): step 1/1.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
CC   -!- PTM: Carbamylation allows a single lysine to coordinate two nickel
CC       ions. {ECO:0000256|PIRSR:PIRSR001222-50}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the metallo-
CC       dependent hydrolases superfamily. Urease alpha subunit family.
CC       {ECO:0000256|PIRNR:PIRNR001222}.
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DR   EMBL; KD283478; EMS45668.1; -; Genomic_DNA.
DR   STRING; 4572.TRIUR3_27202-P1; -.
DR   EnsemblPlants; TRIUR3_27202-T1; TRIUR3_27202-P1; TRIUR3_27202.
DR   Gramene; TRIUR3_27202-T1; TRIUR3_27202-P1; TRIUR3_27202.
DR   OMA; CHHLSHD; -.
DR   OrthoDB; 183108at2759; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000015106; Unassembled WGS sequence.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00375; Urease_alpha; 1.
DR   CDD; cd00407; Urease_beta; 1.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   Gene3D; 2.30.40.10; -; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_01953; Urease_alpha; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR006680; Amidohydro-rel.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR032466; Metal_Hydrolase.
DR   InterPro; IPR008221; Urease.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   InterPro; IPR017950; Urease_AS.
DR   InterPro; IPR005848; Urease_asu.
DR   InterPro; IPR017951; Urease_asu_c.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   InterPro; IPR040881; Urease_linker.
DR   InterPro; IPR029754; Urease_Ni-bd.
DR   Pfam; PF01979; Amidohydro_1; 1.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   Pfam; PF00699; Urease_beta; 1.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   Pfam; PF18473; Urease_linker; 1.
DR   PIRSF; PIRSF001222; Urease; 1.
DR   PRINTS; PR01752; UREASE.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   SUPFAM; SSF51338; SSF51338; 2.
DR   SUPFAM; SSF51556; SSF51556; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR01792; urease_alph; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
DR   PROSITE; PS01120; UREASE_1; 1.
DR   PROSITE; PS00145; UREASE_2; 1.
DR   PROSITE; PS51368; UREASE_3; 1.
PE   3: Inferred from homology;
KW   Complete proteome {ECO:0000313|Proteomes:UP000015106};
KW   Hydrolase {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PROSITE-
KW   ProRule:PRU00700};
KW   Metal-binding {ECO:0000256|PIRNR:PIRNR001222,
KW   ECO:0000256|PIRSR:PIRSR001222-51};
KW   Nickel {ECO:0000256|PIRNR:PIRNR001222, ECO:0000256|PIRSR:PIRSR001222-
KW   51}; Reference proteome {ECO:0000313|Proteomes:UP000015106}.
FT   DOMAIN      379    822       Urease. {ECO:0000259|PROSITE:PS51368}.
FT   ACT_SITE    570    570       Proton donor. {ECO:0000256|PIRSR:
FT                                PIRSR611612-52, ECO:0000256|PROSITE-
FT                                ProRule:PRU00700}.
FT   METAL       384    384       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       386    386       Nickel 1; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       467    467       Nickel 1; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       467    467       Nickel 2; via carbamate group.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       496    496       Nickel 2; via pros nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       522    522       Nickel 2; via tele nitrogen.
FT                                {ECO:0000256|PIRSR:PIRSR001222-51}.
FT   METAL       610    610       Nickel 1. {ECO:0000256|PIRSR:PIRSR001222-
FT                                51}.
FT   BINDING     469    469       Substrate. {ECO:0000256|PROSITE-ProRule:
FT                                PRU00700}.
FT   MOD_RES     467    467       N6-carboxylysine. {ECO:0000256|PIRSR:
FT                                PIRSR001222-50}.
SQ   SEQUENCE   822 AA;  88039 MW;  47A4087028E8E279 CRC64;
     MKLVPREAEK LALHGAGFLA QKRLARGLRL NYTEAIALIA AQILEFVRDG DKTVTDLMDL
     GKQMLGRRQV LPAVPYLLDT VQVQLAYRMS VIQPNTLGVP SLEKFSGSDV EDYPGEVHFC
     SGRIILNLHR RALTLKVVNK ADRPIQIGSH YHFIEANPYL VFDRHRAYGM RLNIPAGTAV
     RFEPGDAKGV TLVSIGGHKV IRGGNGIADG AVDSSQLNEV MQKITENGFG HEDYPDASEG
     LIGDGTFDCS VDHEKYSSMY GPTTGDKIRL GDTDLFAEIE KDFAVYGDEC IFGGGKVLRD
     GMGQSAGYPA SASLDTVITN AVVIDYTGIY KADIGIKDGL IIAIGKAGNP DVMDGVHSNM
     IVGVNTEVIA AQGMIVTAGG IDCHVHFICP QLVNEAIASG ITTLVGGGTG PAHGTCATTC
     TPAPSQMKLM LQSTDEFPIN VGFTGKGNTA KPEGLSEIIM AGAMGLKLHE DWGSTPAVID
     NCLSVAEAFD IQVNIHTDTL NEAGCVEHTI AAFKDRSIHT YHSEGAGGGH APDIIKVCGV
     KNVLPSSTNP TRPFTSNTVD EHLDMLMVCH HLDKNIPEDV AFAESRIRAE TIAGEDILHD
     MGAISIISSD SQAMGRIGEV IIRTWQTANK MKVQRGRLHG PGDSDPAKDN DNFRIRRYIA
     KYTINPAIVS GFSDFVGSVE AGKLADLVLW KPAFFGAKPE LIIKGGAIAW ANMGDPNASI
     PTPEPVMMRP MFGAYGKAGS SHSIAFVSKA AKEAGVASEY KLAKRVEAVG GVRHLSKLDM
     KLNDALPKIE VDPETYTVTA DGEVLTCQPA ATVPLSRNYF LF
//
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