ID M7ZNU9_TRIUA Unreviewed; 799 AA.
AC M7ZNU9;
DT 29-MAY-2013, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2013, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE SubName: Full=O-acyltransferase WSD1 {ECO:0000313|EMBL:EMS61797.1};
GN ORFNames=TRIUR3_35192 {ECO:0000313|EMBL:EMS61797.1};
OS Triticum urartu (Red wild einkorn) (Crithodium urartu).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4572 {ECO:0000313|EMBL:EMS61797.1};
RN [1] {ECO:0000313|EMBL:EMS61797.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23535596; DOI=10.1038/nature11997;
RA Ling H.Q., Zhao S., Liu D., Wang J., Sun H., Zhang C., Fan H., Li D.,
RA Dong L., Tao Y., Gao C., Wu H., Li Y., Cui Y., Guo X., Zheng S., Wang B.,
RA Yu K., Liang Q., Yang W., Lou X., Chen J., Feng M., Jian J., Zhang X.,
RA Luo G., Jiang Y., Liu J., Wang Z., Sha Y., Zhang B., Wu H., Tang D.,
RA Shen Q., Xue P., Zou S., Wang X., Liu X., Wang F., Yang Y., An X., Dong Z.,
RA Zhang K., Zhang X., Luo M.C., Dvorak J., Tong Y., Wang J., Yang H., Li Z.,
RA Wang D., Zhang A., Wang J.;
RT "Draft genome of the wheat A-genome progenitor Triticum urartu.";
RL Nature 496:87-90(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004167}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
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DR EMBL; KD090453; EMS61797.1; -; Genomic_DNA.
DR AlphaFoldDB; M7ZNU9; -.
DR STRING; 4572.M7ZNU9; -.
DR eggNOG; ENOG502QTZ2; Eukaryota.
DR UniPathway; UPA00282; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF67; DIACYLGLYCEROL O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000313|EMBL:EMS61797.1};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EMS61797.1}.
FT DOMAIN 335..559
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 617..785
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
SQ SEQUENCE 799 AA; 90006 MW; 6DA41BFD76F61747 CRC64;
MPSCYRSSYT TSPILLDQEG EDVIELNVCR TRRCRAFGTW IGRIMKTYDY INRVEKRFRL
RCTRKFFEIT TFPNIPRHLF WVVSRAEEED HNKAKVHSAA ERGNSALVAK LVKENPQLLT
QNNTFFESLE DACKIEWECE QIANPNTELF HEFDVELFDE EEKVESPTTN DQGARGLMNI
SSNYLAVMLI TRRLHPTLYA RSNPNLRANT SAVKTSAQPI NFPVSANNEF SIVISENSPS
RASKQIVIKR SIDIQFNKSH RSCSPSGAAL PTTRLLPIRT QRSAVAEWTT TDYSATAAAA
EEEPLSPTAR LMETIHIIVT VGLGCPVNLP VFSAGIAAQL ARYPRFRSIQ VTDGSKGPRW
ARTAVNVDDH MIVPTLHPVA VEADPDRALE DYVASLYTLP MDRSRPLWEF HFLDFPTSEA
ASTAVIRLHH SLGDGMSLIT LLLASARSVA DPTRLPAMPE QPARTGAIYV PRRRQPPAAG
VLAAFVTWIW SYLVLVWNTM VDFAIFAVTI VFRRDLHTLF KRVDGDVTSN RSRRFVHRSL
SLDDVKLIKN VMNCTVNDVL VGATSAALSR YYFRKSGSSK ASKLCLRSVL PVNTRPTTSL
QTYVDMIESG KSNDVAWGNQ LGYILLPFHL AMHNDPLAYV RKAKKIIDRK KSSLEVIFTC
KMNAFFVKMF GMKSSTGTQL SSLHLVVMEV HMRNYNIQFK EHSNKVRCTL TPITSGDFTI
MRIDGSPIKE MTYLGWAYTL PTKALAVQYQ SYDNTIKVIL SVDDEVIPDY AQLLDDFTES
FGRIKDASSR LSASIIKKE
//
