ID M9NFI2_DROME Unreviewed; 1817 AA.
AC M9NFI2;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 24-JAN-2024, entry version 73.
DE SubName: Full=Spinophilin, isoform E {ECO:0000313|EMBL:AFH04244.1};
GN Name=Spn {ECO:0000313|EMBL:AFH04244.1,
GN ECO:0000313|FlyBase:FBgn0010905};
GN Synonyms=BcDNA:GM16129 {ECO:0000313|EMBL:AFH04244.1}, c62E-5
GN {ECO:0000313|EMBL:AFH04244.1}, Dmel\CG16757
GN {ECO:0000313|EMBL:AFH04244.1}, E62 {ECO:0000313|EMBL:AFH04244.1}, EL62
GN {ECO:0000313|EMBL:AFH04244.1}, l(3)06911
GN {ECO:0000313|EMBL:AFH04244.1}, spn {ECO:0000313|EMBL:AFH04244.1};
GN ORFNames=CG16757 {ECO:0000313|EMBL:AFH04244.1,
GN ECO:0000313|FlyBase:FBgn0010905}, Dmel_CG16757
GN {ECO:0000313|EMBL:AFH04244.1};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803};
RN [1] {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.H., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Gabor G.L., Abril J.F., Agbayani A.,
RA An H.J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D., Scheeler F., Shen H.,
RA Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T., Spier E.,
RA Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C.,
RA Turner R., Venter E., Wang A.H., Wang X., Wang Z.Y., Wassarman D.A.,
RA Weinstock G.M., Weissenbach J., Williams S.M., WoodageT, Worley K.C.,
RA Wu D., Yang S., Yao Q.A., Ye J., Yeh R.F., Zaveri J.S., Zhan M., Zhang G.,
RA Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.,
RA Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537568;
RA Celniker S.E., Wheeler D.A., Kronmiller B., Carlson J.W., Halpern A.,
RA Patel S., Adams M., Champe M., Dugan S.P., Frise E., Hodgson A.,
RA George R.A., Hoskins R.A., Laverty T., Muzny D.M., Nelson C.R.,
RA Pacleb J.M., Park S., Pfeiffer B.D., Richards S., Sodergren E.J.,
RA Svirskas R., Tabor P.E., Wan K., Stapleton M., Sutton G.G., Venter C.,
RA Weinstock G., Scherer S.E., Myers E.W., Gibbs R.A., Rubin G.M.;
RT "Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster
RT euchromatic genome sequence.";
RL Genome Biol. 3:RESEARCH0079-RESEARCH0079(2002).
RN [3] {ECO:0000313|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537573;
RA Kaminker J.S., Bergman C.M., Kronmiller B., Carlson J., Svirskas R.,
RA Patel S., Frise E., Wheeler D.A., Lewis S.E., Rubin G.M., Ashburner M.,
RA Celniker S.E.;
RT "The transposable elements of the Drosophila melanogaster euchromatin: a
RT genomics perspective.";
RL Genome Biol. 3:RESEARCH0084.1-RESEARCH0084.20(2002).
RN [5] {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=12537574;
RA Hoskins R.A., Smith C.D., Carlson J.W., Carvalho A.B., Halpern A.,
RA Kaminker J.S., Kennedy C., Mungall C.J., Sullivan B.A., Sutton G.G.,
RA Yasuhara J.C., Wakimoto B.T., Myers E.W., Celniker S.E., Rubin G.M.,
RA Karpen G.H.;
RT "Heterochromatic sequences in a Drosophila whole-genome shotgun assembly.";
RL Genome Biol. 3:RESEARCH0085-RESEARCH0085(2002).
RN [6] {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=16110336; DOI=10.1371/journal.pcbi.0010022;
RA Quesneville H., Bergman C.M., Andrieu O., Autard D., Nouaud D.,
RA Ashburner M., Anxolabehere D.;
RT "Combined evidence annotation of transposable elements in genome
RT sequences.";
RL PLoS Comput. Biol. 1:166-175(2005).
RN [7] {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569856; DOI=10.1126/science.1139815;
RA Smith C.D., Shu S., Mungall C.J., Karpen G.H.;
RT "The Release 5.1 annotation of Drosophila melanogaster heterochromatin.";
RL Science 316:1586-1591(2007).
RN [8] {ECO:0000313|EMBL:AFH04244.1, ECO:0000313|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000313|Proteomes:UP000000803};
RX PubMed=17569867; DOI=10.1126/science.1139816;
RA Hoskins R.A., Carlson J.W., Kennedy C., Acevedo D., Evans-Holm M.,
RA Frise E., Wan K.H., Park S., Mendez-Lago M., Rossi F., Villasante A.,
RA Dimitri P., Karpen G.H., Celniker S.E.;
RT "Sequence finishing and mapping of Drosophila melanogaster
RT heterochromatin.";
RL Science 316:1625-1628(2007).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton
CC {ECO:0000256|ARBA:ARBA00004245}.
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DR EMBL; AE014296; AFH04244.1; -; Genomic_DNA.
DR RefSeq; NP_001246573.1; NM_001259644.1.
DR SMR; M9NFI2; -.
DR EnsemblMetazoa; FBtr0304616; FBpp0293158; FBgn0010905.
DR GeneID; 46194; -.
DR AGR; FB:FBgn0010905; -.
DR CTD; 6693; -.
DR FlyBase; FBgn0010905; Spn.
DR VEuPathDB; VectorBase:FBgn0010905; -.
DR GeneTree; ENSGT00940000169407; -.
DR HOGENOM; CLU_001722_0_0_1; -.
DR OrthoDB; 2911512at2759; -.
DR BioGRID-ORCS; 46194; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 46194; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0010905; Expressed in eye disc (Drosophila) and 24 other cell types or tissues.
DR ExpressionAtlas; M9NFI2; baseline and differential.
DR GO; GO:0015629; C:actin cytoskeleton; IBA:GO_Central.
DR GO; GO:0045179; C:apical cortex; HDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0030425; C:dendrite; IBA:GO_Central.
DR GO; GO:0014069; C:postsynaptic density; IBA:GO_Central.
DR GO; GO:0051015; F:actin filament binding; IBA:GO_Central.
DR GO; GO:0042043; F:neurexin family protein binding; IPI:FlyBase.
DR GO; GO:0007015; P:actin filament organization; IBA:GO_Central.
DR GO; GO:0019722; P:calcium-mediated signaling; IBA:GO_Central.
DR GO; GO:1905519; P:negative regulation of presynaptic active zone assembly; IMP:FlyBase.
DR GO; GO:0031175; P:neuron projection development; IBA:GO_Central.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd09512; SAM_Neurabin-like; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 1.10.150.50; Transcription Factor, Ets-1; 1.
DR InterPro; IPR040645; Neurabin-1/2_PDZ.
DR InterPro; IPR043446; Neurabin-like.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR001660; SAM.
DR InterPro; IPR013761; SAM/pointed_sf.
DR PANTHER; PTHR16154; NEURABIN; 1.
DR PANTHER; PTHR16154:SF6; SPINOPHILIN, ISOFORM J; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF17817; PDZ_5; 1.
DR Pfam; PF00536; SAM_1; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00454; SAM; 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF47769; SAM/Pointed domain; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50105; SAM_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Proteomics identification {ECO:0007829|PeptideAtlas:M9NFI2};
KW Reference proteome {ECO:0000313|Proteomes:UP000000803}.
FT DOMAIN 1261..1349
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 1725..1769
FT /note="SAM"
FT /evidence="ECO:0000259|PROSITE:PS50105"
FT REGION 89..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 183..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 369..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 656..722
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 956..990
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1016..1038
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1385..1410
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1656..1704
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1787..1817
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1457..1519
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 99..113
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 114..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 273..294
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..419
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 430..463
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..480
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..505
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 506..546
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 554..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 657..681
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 696..722
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 956..973
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1016..1031
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1388..1407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1787..1808
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1817 AA; 198356 MW; 578F00E42E966340 CRC64;
MEKPMHHAPA PVGKVSQIAN IFQRKPIEIQ PVEQLSAVAA AHAAAAAAAA AAHHAHVQGA
PAVRTESHSA RFNNARALFE KLGVESNSNV SSRLLRSGSR EDNLCDGSDR SSSRSSDRSQ
SPPKRRTPFP SGVSLVHNNN NAAIVAQNGV PPEQRLSNSK FIVEPAAQVV PTSVVKYPQH
NISRLKSEEP SPVPPPASGS VSALFASSGG DKPEKPERKF NSRELIEKQK KWTSHFTKTK
TTRTHSDLNR CDIIRTVPGT GLIMDSEKVA KPAMEPPQPP PNASPNPPMR AQAPPEIKPR
SGKIGSPVKS PPLPPIPAVK PKNVSPVKFN PDRLRQSPTK TADNSPPPPP AKSAAVLQRS
LMQEQQELLR NSCDQGVAPI PPEKPRKKSV DLIEDTLPLT NCSTPSSCAS PTSSYLMQPA
KRGSLDGGSG NGQYPGNGLS GSTNSATSGS PVASASSGPS SPVHTEDEKQ ENESTEKSEM
EYYHGGNYNS VPRRRRSENE GRKSVDESSP SANNSQQQQQ HSIPGSAAGS PQRVANKRSS
ITVNMPAAGL GQRPPSIIST TSQDEGGFNE SAPELKAKLQ PAYDQTEEQP HSLNYVDVGY
RLNPDGSESR EVYGSEAELY DTAKVTDMQR KFHGANGFGQ ESSTVYAIIK PDVQESQPVA
PSRSVLIQSP NSSSVEGSPL HRGSYSSPPV GVVSPIRRRN SSNQDQSVGG GGSAKTTPQC
SPARSALVKG IAPIASIDAH EEEELDLVEE DEHLAVEYVE VLELQQDEEE EEAPVLPERR
APAQGSLELQ DLEYADTSAG EDEEDIINHL KDGDVLDVEL IDDVVDEVIK VHVNHSVATA
PSIQAATPAA AIPREDSLPD DMTAAEAERL LSSSILENKI RQQSLLSDEQ AKEVEQILNA
APSVGVAVAT VVATATSPTS IKNLIEDLPG QSAVAASAAN GEQDIQIAAV PAIVEEDEDE
EEDFPEDDEE DHARADFDAN GGDADGDSDD VEAVDIVGYG HASTALNATF VKADSTETET
TTTTPSTATT ATTRHDDDEP EWLRDVLEAP KRSLENLLIT SATSSRAPGQ REELENGYDL
HEKHSDLNQT YITGGESLHE SIVSVESTQS DATLNQTTTI DDSIISSKHN STYSLADAEQ
ATSSTVLSTG VTELDDSQYY IPEYPPVRSK EVLVEAGVHY FEDGNFWMEV PGLLDFDDDD
CSYPPITVRK NPKVRFSSGP IHVYSTFSVN DYDRRNEDVD PVAASAEYEL EKRVEKMHVF
PVELMKGPEG LGLSIIGMGV GADAGLEKLG IFVKTITDNG AAARDGRIQV NDQIIEVDGK
SLVGVTQAYA ASVLRNTSGL VKFQIGRERD PENSEVAQLI RLSLQADREK EERLKRQQEE
YLRRTLDYSE DSTQPVSANS SVCEGPSSPV QVEHPMEVEA THSQEVESLK RLLQESEMGC
LVKEEIIQNL KRKLVKLETT GNENELLSER LRQSERELGN IRKEAANLQN MLQQSQGQYM
ALDKKYNKAK RLVREYQQRE LDMCHREEFY QQLLQEKDTE YNALVKKLKD RVINLEHELQ
ETQRKAGFPV GLPYDSATLK LTPQMMRKTP PKPLFHKLET ELSDTEISDL SPDGDGVKTA
TVERKVPVKD ELDAAVPQHE LLDNSINKTK IDLVPWQQQH HQQIQQQPSA HTTGPPSPTS
MSSGCSSPGY SPSRTLDLSG SSSSFSDRKA MAAGYTYKGG PVHEWTKDQV GHWLMGIELE
RYIPVFKENN VEGGALLTLD SKDFKTLGIC GDDKHRLKKR LKDLKANIEK ERKDMERERR
EREKAIRKAE KKAAKKK
//
