ID MAL2_MOUSE Reviewed; 175 AA.
AC Q8BI08;
DT 12-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 24-JAN-2024, entry version 139.
DE RecName: Full=Protein MAL2;
GN Name=Mal2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP PROTEIN SEQUENCE OF 22-32, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
CC -!- FUNCTION: Member of the machinery of polarized transport. Required for
CC the indirect transcytotic route at the step of the egress of the
CC transcytosing cargo from perinuclear endosomes in order for it to
CC travel to the apical surface via a raft-dependent pathway (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with TPD52L2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Apical cell membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Note=Associated with lipid rafts. In
CC polarized epithelial cells, restricted to the apical surface (By
CC similarity). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the MAL family. {ECO:0000305}.
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DR EMBL; AK035986; BAC29268.1; -; mRNA.
DR CCDS; CCDS27470.1; -.
DR RefSeq; NP_849251.1; NM_178920.4.
DR AlphaFoldDB; Q8BI08; -.
DR BioGRID; 222936; 1.
DR STRING; 10090.ENSMUSP00000025356; -.
DR GlyCosmos; Q8BI08; 1 site, No reported glycans.
DR GlyGen; Q8BI08; 1 site.
DR iPTMnet; Q8BI08; -.
DR PhosphoSitePlus; Q8BI08; -.
DR SwissPalm; Q8BI08; -.
DR jPOST; Q8BI08; -.
DR MaxQB; Q8BI08; -.
DR PaxDb; 10090-ENSMUSP00000025356; -.
DR ProteomicsDB; 292083; -.
DR Antibodypedia; 26765; 45 antibodies from 9 providers.
DR DNASU; 105853; -.
DR Ensembl; ENSMUST00000025356.4; ENSMUSP00000025356.3; ENSMUSG00000024479.4.
DR GeneID; 105853; -.
DR KEGG; mmu:105853; -.
DR UCSC; uc007vrm.2; mouse.
DR AGR; MGI:2146021; -.
DR CTD; 114569; -.
DR MGI; MGI:2146021; Mal2.
DR VEuPathDB; HostDB:ENSMUSG00000024479; -.
DR eggNOG; KOG4788; Eukaryota.
DR GeneTree; ENSGT00940000159514; -.
DR HOGENOM; CLU_112950_2_0_1; -.
DR InParanoid; Q8BI08; -.
DR OMA; GFKRWRM; -.
DR OrthoDB; 5356963at2759; -.
DR PhylomeDB; Q8BI08; -.
DR TreeFam; TF316174; -.
DR BioGRID-ORCS; 105853; 1 hit in 77 CRISPR screens.
DR ChiTaRS; Mal2; mouse.
DR PRO; PR:Q8BI08; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q8BI08; Protein.
DR Bgee; ENSMUSG00000024479; Expressed in subiculum and 187 other cell types or tissues.
DR ExpressionAtlas; Q8BI08; baseline and differential.
DR Genevisible; Q8BI08; MM.
DR GO; GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0098686; C:hippocampal mossy fiber to CA3 synapse; ISO:MGI.
DR GO; GO:0045121; C:membrane raft; ISO:MGI.
DR GO; GO:0030672; C:synaptic vesicle membrane; ISO:MGI.
DR GO; GO:0019911; F:structural constituent of myelin sheath; IBA:GO_Central.
DR GO; GO:0042552; P:myelination; IBA:GO_Central.
DR InterPro; IPR013295; MAL.
DR InterPro; IPR008253; Marvel.
DR PANTHER; PTHR22776; MARVEL-CONTAINING POTENTIAL LIPID RAFT-ASSOCIATED PROTEIN; 1.
DR PANTHER; PTHR22776:SF42; PROTEIN MAL2; 1.
DR Pfam; PF01284; MARVEL; 1.
DR PRINTS; PR01884; MALPROTEIN.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..175
FT /note="Protein MAL2"
FT /id="PRO_0000156809"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..65
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..101
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..148
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..175
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 30..174
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 175 AA; 19094 MW; CE28C15CD9B6FA15 CRC64;
MSAGGAVPPP PNPAVSFPAP RVTLPAGPDI LRTYSGAFVC LEIVLGGLVW ILVASSNVPL
PLLQGWVMFV SVTAFFFSLL FLGLFLSGMV TQIDANWNFL DFVYHFIVFV FYFGAFLLEA
AATSLHDLQC NTTMTVKPLL NDNQYNINVA ATVFAFMTTA CYGCSLGLAL RRWRP
//
