ID MCA8_ARATH Reviewed; 381 AA.
AC Q9SA41; Q683B3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 24-JAN-2024, entry version 122.
DE RecName: Full=Metacaspase-8;
DE Short=AtMC8;
DE EC=3.4.22.-;
DE AltName: Full=Metacaspase 2e;
DE Short=AtMCP2e;
GN Name=AMC8; Synonyms=MCP2E; OrderedLocusNames=At1g16420; ORFNames=F3O9.22;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AUTOCATALYTIC CLEAVAGE, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=15326173; DOI=10.1074/jbc.m406329200;
RA Vercammen D., van de Cotte B., De Jaeger G., Eeckhout D., Casteels P.,
RA Vandepoele K., Vandenberghe I., van Beeumen J., Inze D., van Breusegem F.;
RT "Type II metacaspases Atmc4 and Atmc9 of Arabidopsis thaliana cleave
RT substrates after arginine and lysine.";
RL J. Biol. Chem. 279:45329-45336(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Ikeda Y., Krishnamurthy N., Chua N.-H.;
RT "Characterization of metacaspases.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=20565583; DOI=10.1111/j.1364-3703.2004.00206.x;
RA Watanabe N., Lam E.;
RT "Recent advance in the study of caspase-like proteases and Bax inhibitor-1
RT in plants: their possible roles as regulator of programmed cell death.";
RL Mol. Plant Pathol. 5:65-70(2004).
RN [7]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, INDUCTION, AND MUTAGENESIS OF
RP CYS-140.
RX PubMed=17998208; DOI=10.1074/jbc.m704185200;
RA He R., Drury G.E., Rotari V.I., Gordon A., Willer M., Farzaneh T.,
RA Woltering E.J., Gallois P.;
RT "Metacaspase-8 modulates programmed cell death induced by ultraviolet light
RT and H2O2 in Arabidopsis.";
RL J. Biol. Chem. 283:774-783(2008).
CC -!- FUNCTION: Cysteine protease that cleaves specifically after arginine
CC residues. Does not cleave caspase-specific substrates. May be involved
CC in the modulation of programmed cell death activated by oxidative
CC stress. {ECO:0000269|PubMed:17998208}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.5-8.5 with t-butoxycarbonyl-GRR-aminomethylcoumarin
CC as substrate. {ECO:0000269|PubMed:17998208};
CC -!- INDUCTION: By UV-C light, hydrogen peroxide and methyl viologen.
CC {ECO:0000269|PubMed:17998208}.
CC -!- PTM: Proteolytically processed; by an autocatalytic mechanism.
CC -!- SIMILARITY: Belongs to the peptidase C14B family. {ECO:0000305}.
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DR EMBL; AY219833; AAP44521.1; -; mRNA.
DR EMBL; AY322530; AAP84711.1; -; mRNA.
DR EMBL; AC006341; AAD34694.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29449.1; -; Genomic_DNA.
DR EMBL; AK175204; BAD42967.1; -; mRNA.
DR PIR; E86299; E86299.
DR RefSeq; NP_173092.1; NM_101508.4.
DR AlphaFoldDB; Q9SA41; -.
DR SMR; Q9SA41; -.
DR BioGRID; 23452; 1.
DR STRING; 3702.Q9SA41; -.
DR MEROPS; C14.046; -.
DR iPTMnet; Q9SA41; -.
DR PaxDb; 3702-AT1G16420-1; -.
DR EnsemblPlants; AT1G16420.1; AT1G16420.1; AT1G16420.
DR GeneID; 838212; -.
DR Gramene; AT1G16420.1; AT1G16420.1; AT1G16420.
DR KEGG; ath:AT1G16420; -.
DR Araport; AT1G16420; -.
DR TAIR; AT1G16420; MC8.
DR eggNOG; KOG1546; Eukaryota.
DR HOGENOM; CLU_029389_4_1_1; -.
DR InParanoid; Q9SA41; -.
DR OMA; TGQTNIE; -.
DR OrthoDB; 555417at2759; -.
DR PhylomeDB; Q9SA41; -.
DR PRO; PR:Q9SA41; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SA41; baseline and differential.
DR Genevisible; Q9SA41; AT.
DR GO; GO:0004197; F:cysteine-type endopeptidase activity; IDA:TAIR.
DR GO; GO:0010421; P:hydrogen peroxide-mediated programmed cell death; IMP:TAIR.
DR GO; GO:0012501; P:programmed cell death; IMP:TAIR.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR GO; GO:0042542; P:response to hydrogen peroxide; IMP:TAIR.
DR GO; GO:0006979; P:response to oxidative stress; IMP:TAIR.
DR GO; GO:0010225; P:response to UV-C; IEP:TAIR.
DR Gene3D; 3.40.50.12660; -; 2.
DR InterPro; IPR011600; Pept_C14_caspase.
DR PANTHER; PTHR48104; METACASPASE-4; 1.
DR PANTHER; PTHR48104:SF18; METACASPASE-8; 1.
DR Pfam; PF00656; Peptidase_C14; 1.
PE 1: Evidence at protein level;
KW Autocatalytic cleavage; Hydrolase; Protease; Reference proteome;
KW S-nitrosylation; Thiol protease.
FT CHAIN 1..381
FT /note="Metacaspase-8"
FT /id="PRO_0000334606"
FT ACT_SITE 86
FT /evidence="ECO:0000250"
FT ACT_SITE 140
FT MOD_RES 140
FT /note="S-nitrosocysteine"
FT /evidence="ECO:0000250|UniProtKB:Q9FYE1"
FT MUTAGEN 140
FT /note="C->A: Loss of protease activity."
FT /evidence="ECO:0000269|PubMed:17998208"
FT CONFLICT 99
FT /note="D -> G (in Ref. 5; BAD42967)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 381 AA; 42521 MW; 75131FA680035E2A CRC64;
MAKKALLIGI NYPGTAVELR GCVNDVHRMQ KCLIELYGFA NKDIVIMIDT DKSCIQPTGK
NICDELDNLI ASGQSGDFLV FHYSGHGTRI PPGIEDSEDP TGFDECITPC DMNLIKDQQF
REMVSRVKEG CQLTIISDSC HSGGLIQEVK EQIGESHMKP VDKVKEQIEE SHMKQPKLGI
ASYFLNIVMN LLATCGVSKS QRDRGGGEES FRGEIELEKD ETLDIKTRYL PFESYLSLLK
EQTGQTNIEP VRIRQTLLKL FGEDPSPNRQ RGLSDLGNCE VDAGDSGASR LNAVTDNGIL
LSGCQTDQRS EDVYVTRTGK AYGAFSDAIQ MILSAPRKDK KKITNKELVS EARVFLKKRG
YSQRPGLYCH DRFVDKPFIC Y
//
