ID MDTO_ECOLI Reviewed; 683 AA.
AC P32715; Q2M6M3;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 15-JUL-1998, sequence version 2.
DT 27-MAR-2024, entry version 165.
DE RecName: Full=Multidrug resistance protein MdtO;
GN Name=mdtO; Synonyms=yjcQ; OrderedLocusNames=b4081, JW4042;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PUTATIVE FUNCTION.
RX PubMed=11257026; DOI=10.1128/aac.45.4.1126-1136.2001;
RA Sulavik M.C., Houseweart C., Cramer C., Jiwani N., Murgolo N., Greene J.,
RA DiDomenico B., Shaw K.J., Miller G.H., Hare R., Shimer G.;
RT "Antibiotic susceptibility profiles of Escherichia coli strains lacking
RT multidrug efflux pump genes.";
RL Antimicrob. Agents Chemother. 45:1126-1136(2001).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP OPERON STRUCTURE, AND INDUCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=19429622; DOI=10.1128/jb.00108-09;
RA Shimada T., Yamamoto K., Ishihama A.;
RT "Involvement of the leucine response transcription factor LeuO in
RT regulation of the genes for sulfa drug efflux.";
RL J. Bacteriol. 191:4562-4571(2009).
CC -!- FUNCTION: Could be involved in resistance to puromycin, acriflavine and
CC tetraphenylarsonium chloride.
CC -!- SUBUNIT: Could be part of a tripartite efflux system composed of MdtN,
CC MdtO and MdtP.
CC -!- INTERACTION:
CC P32715; P76237: dgcJ; NbExp=2; IntAct=EBI-555775, EBI-555763;
CC P32715; P07395: pheT; NbExp=4; IntAct=EBI-555775, EBI-555713;
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000305|PubMed:15919996}; Multi-pass membrane protein
CC {ECO:0000305|PubMed:15919996}.
CC -!- INDUCTION: Induced by LeuO, part of the mdtNO operon.
CC {ECO:0000269|PubMed:19429622}.
CC -!- SIMILARITY: Belongs to the MdtO family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC43175.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00006; AAC43175.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAD13464.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE78083.1; -; Genomic_DNA.
DR PIR; H65216; H65216.
DR RefSeq; NP_418505.2; NC_000913.3.
DR RefSeq; WP_001275207.1; NZ_LN832404.1.
DR AlphaFoldDB; P32715; -.
DR BioGRID; 4262952; 6.
DR BioGRID; 852876; 3.
DR ComplexPortal; CPX-6021; SdsRQP multidrug transport complex.
DR DIP; DIP-12556N; -.
DR IntAct; P32715; 3.
DR STRING; 511145.b4081; -.
DR TCDB; 2.A.85.6.1; the aromatic acid exporter (arae) family.
DR PaxDb; 511145-b4081; -.
DR EnsemblBacteria; AAD13464; AAD13464; b4081.
DR GeneID; 948582; -.
DR KEGG; ecj:JW4042; -.
DR KEGG; eco:b4081; -.
DR PATRIC; fig|1411691.4.peg.2620; -.
DR EchoBASE; EB1896; -.
DR eggNOG; COG1289; Bacteria.
DR HOGENOM; CLU_023392_1_0_6; -.
DR InParanoid; P32715; -.
DR OMA; WPGIHTC; -.
DR OrthoDB; 105720at2; -.
DR BioCyc; EcoCyc:YJCQ-MONOMER; -.
DR BioCyc; MetaCyc:YJCQ-MONOMER; -.
DR PRO; PR:P32715; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:1990351; C:transporter complex; NAS:ComplexPortal.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; NAS:ComplexPortal.
DR InterPro; IPR006726; PHBA_efflux_AaeB/fusaric-R.
DR PANTHER; PTHR30509:SF44; INNER MEMBRANE PROTEIN YEEA-RELATED; 1.
DR PANTHER; PTHR30509; P-HYDROXYBENZOIC ACID EFFLUX PUMP SUBUNIT-RELATED; 1.
DR Pfam; PF04632; FUSC; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..683
FT /note="Multidrug resistance protein MdtO"
FT /id="PRO_0000210089"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 426..446
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 457..477
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 683 AA; 76151 MW; F9BE67EE4E01921C CRC64;
MSALNSLPLP VVRLLAFFHE ELSERRPGRV PQTVQLWVGC LLVILISMTF EIPFVALSLA
VLFYGIQSNA FYTKFVAILF VVATVLEIGS LFLIYKWSYG EPLIRLIIAG PILMGCMFLM
RTHRLGLVFF AVAIVAIYGQ TFPAMLDYPE VVVRLTLWCI VVGLYPTLLM TLIGVLWFPS
RAISQMHQAL NDRLDDAISH LTDSLAPLPE TRIEREALAL QKLNVFCLAD DANWRTQNAW
WQSCVATVTY IYSTLNRYDP TSFADSQAII EFRQKLASEI NKLQHAVAEG QCWQSDWRIS
ESEAMAAREC NLENICQTLL QLGQMDPNTP PTPAAKPPSM AADAFTNPDY MRYAVKTLLA
CLICYTFYSG VDWEGIHTCM LTCVIVANPN VGSSYQKMVL RFGGAFCGAI LALLFTLLVM
PWLDNIVELL FVLAPIFLLG AWIATSSERS SYIGTQMVVT FALATLENVF GPVYDLVEIR
DRALGIIIGT VVSAVIYTFV WPESEARTLP QKLAGTLGML SKVMRIPRQQ EVTALRTYLQ
IRIGLHAAFN ACEEMCQRVA LERQLDSEER ALLIERSQTV IRQGRDLLHA WDATWNSAQA
LDNALQPDRA GQFADALEKY AAGLATALSR SPQITLEETP ASQAILPTLL KQEQHVCQLF
ARLPDWTAPA LTPATEQAQG ATQ
//
