ID MEXR_PSEAE Reviewed; 147 AA.
AC P52003; Q5EEX3; Q5EEX4; Q5EEX5; Q83UE4;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 27-MAR-2024, entry version 144.
DE RecName: Full=Multidrug resistance operon repressor;
GN Name=mexR; OrderedLocusNames=PA0424;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANT TRP-70.
RC STRAIN=PAO;
RX PubMed=8878574; DOI=10.1128/aac.40.9.2021;
RA Poole K., Tetro K., Zhao Q., Neshat S., Heinrichs D.E., Bianco N.;
RT "Expression of the multidrug resistance operon mexA-mexB-oprM in
RT Pseudomonas aeruginosa: mexR encodes a regulator of operon expression.";
RL Antimicrob. Agents Chemother. 40:2021-2028(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=55699, 58823, 65629, and GRR;
RA Ho S.E., Navaratnam P.;
RT "Mutation in MexAB-OprM regulator gene, MexR contribute to the carbapenem
RT resistance in Pseudomonas aeruginosa.";
RL Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PA36, PA41, and PA48;
RA Yi M., Wang P., Liu Y., Huang H.;
RT "Mutation in MexAB-OprM regulator gene, MexR contributes to the multidrug
RT resistance in Pseudomonas aeruginosa.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS), AND SUBUNIT.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=12034710; DOI=10.1074/jbc.m111381200;
RA Lim D., Poole K., Strynadka N.C.J.;
RT "Crystal structure of the MexR repressor of the mexRAB-oprM multidrug
RT efflux operon of Pseudomonas aeruginosa.";
RL J. Biol. Chem. 277:29253-29259(2002).
CC -!- FUNCTION: Repressor of the mexAB-oprM multidrug resistance operon. Also
CC represses its own expression. Many variants lead to increased
CC expression of the mexAB-oprM operon.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12034710}.
CC -!- INTERACTION:
CC P52003; Q9HXS2: armR; NbExp=2; IntAct=EBI-6400193, EBI-6400203;
CC P52003; P52003: mexR; NbExp=2; IntAct=EBI-6400193, EBI-6400193;
CC P52003; Q8GQ91: armR; Xeno; NbExp=5; IntAct=EBI-6400193, EBI-15730301;
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DR EMBL; U23763; AAA69479.1; -; Genomic_DNA.
DR EMBL; AY190292; AAO40255.1; -; Genomic_DNA.
DR EMBL; AY190294; AAO40257.1; -; Genomic_DNA.
DR EMBL; AY190293; AAO40256.1; -; Genomic_DNA.
DR EMBL; AY190295; AAO40258.1; -; Genomic_DNA.
DR EMBL; AY899299; AAW82614.1; -; Genomic_DNA.
DR EMBL; AY899300; AAW82615.1; -; Genomic_DNA.
DR EMBL; AY899301; AAW82616.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG03813.1; -; Genomic_DNA.
DR PIR; C83593; C83593.
DR RefSeq; NP_249115.1; NC_002516.2.
DR RefSeq; WP_003114897.1; NZ_QZGE01000016.1.
DR PDB; 1LNW; X-ray; 2.10 A; A/B/C/D/E/F/G/H=1-147.
DR PDB; 3ECH; X-ray; 1.80 A; A/B=1-142.
DR PDB; 3MEX; X-ray; 2.10 A; A/B=1-142.
DR PDB; 4ZZL; X-ray; 2.19 A; A/B=5-139.
DR PDBsum; 1LNW; -.
DR PDBsum; 3ECH; -.
DR PDBsum; 3MEX; -.
DR PDBsum; 4ZZL; -.
DR AlphaFoldDB; P52003; -.
DR SASBDB; P52003; -.
DR SMR; P52003; -.
DR DIP; DIP-46302N; -.
DR IntAct; P52003; 2.
DR MINT; P52003; -.
DR STRING; 208964.PA0424; -.
DR PaxDb; 208964-PA0424; -.
DR DNASU; 877857; -.
DR GeneID; 877857; -.
DR KEGG; pae:PA0424; -.
DR PATRIC; fig|208964.12.peg.446; -.
DR PseudoCAP; PA0424; -.
DR HOGENOM; CLU_083287_18_6_6; -.
DR InParanoid; P52003; -.
DR OrthoDB; 6196575at2; -.
DR PhylomeDB; P52003; -.
DR BioCyc; PAER208964:G1FZ6-428-MONOMER; -.
DR EvolutionaryTrace; P52003; -.
DR PHI-base; PHI:11921; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0032993; C:protein-DNA complex; IPI:CollecTF.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IPI:CollecTF.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:CollecTF.
DR GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:PseudoCAP.
DR GO; GO:0050709; P:negative regulation of protein secretion; IMP:PseudoCAP.
DR GO; GO:0034763; P:negative regulation of transmembrane transport; IMP:PseudoCAP.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR000835; HTH_MarR-typ.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR35790; HTH-TYPE TRANSCRIPTIONAL REGULATOR PCHR; 1.
DR PANTHER; PTHR35790:SF4; HTH-TYPE TRANSCRIPTIONAL REGULATOR PCHR; 1.
DR Pfam; PF01047; MarR; 1.
DR PRINTS; PR00598; HTHMARR.
DR SMART; SM00347; HTH_MARR; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS50995; HTH_MARR_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..147
FT /note="Multidrug resistance operon repressor"
FT /id="PRO_0000054365"
FT DOMAIN 6..140
FT /note="HTH marR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00345"
FT VARIANT 69
FT /note="T -> A (in strain: PA48)"
FT VARIANT 70
FT /note="R -> W (in strain: OCR1; leads to increased
FT expression of mexAB-oprM)"
FT /evidence="ECO:0000269|PubMed:8878574"
FT VARIANT 95
FT /note="L -> F (in strain: PA41)"
FT VARIANT 126
FT /note="V -> E (in strain: 58823, 65629, 55699 and GRR)"
FT VARIANT 130
FT /note="T -> S (in strain: PA36)"
FT HELIX 9..30
FT /evidence="ECO:0007829|PDB:3ECH"
FT HELIX 37..48
FT /evidence="ECO:0007829|PDB:3ECH"
FT TURN 49..51
FT /evidence="ECO:0007829|PDB:3ECH"
FT HELIX 54..61
FT /evidence="ECO:0007829|PDB:3ECH"
FT HELIX 67..77
FT /evidence="ECO:0007829|PDB:3ECH"
FT STRAND 80..82
FT /evidence="ECO:0007829|PDB:3ECH"
FT STRAND 87..97
FT /evidence="ECO:0007829|PDB:1LNW"
FT HELIX 99..120
FT /evidence="ECO:0007829|PDB:3ECH"
FT HELIX 125..140
FT /evidence="ECO:0007829|PDB:3ECH"
SQ SEQUENCE 147 AA; 16965 MW; 59F5146394BE89DD CRC64;
MNYPVNPDLM PALMAVFQHV RTRIQSELDC QRLDLTPPDV HVLKLIDEQR GLNLQDLGRQ
MCRDKALITR KIRELEGRNL VRRERNPSDQ RSFQLFLTDE GLAIHQHAEA IMSRVHDELF
APLTPVEQAT LVHLLDQCLA AQPLEDI
//
