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Database: UniProt
Entry: MFAL1_YEAST
LinkDB: MFAL1_YEAST
Original site: MFAL1_YEAST 
ID   MFAL1_YEAST             Reviewed;         165 AA.
AC   P01149; D6W3I2; P25041;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   12-AUG-2020, entry version 149.
DE   RecName: Full=Mating factor alpha-1;
DE   AltName: Full=Alpha-1 mating pheromone;
DE   Contains:
DE     RecName: Full=Mating factor alpha;
DE   Flags: Precursor;
GN   Name=MF(ALPHA)1; Synonyms=MF-ALPHA-1, MFAL1; OrderedLocusNames=YPL187W;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6306574; DOI=10.1093/nar/11.12.4049;
RA   Singh A., Chen E.Y., Lugovoy J.M., Chang C.N., Hitzeman R.A., Seeburg P.H.;
RT   "Saccharomyces cerevisiae contains two discrete genes coding for the alpha-
RT   factor pheromone.";
RL   Nucleic Acids Res. 11:4049-4063(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6754095; DOI=10.1016/0092-8674(82)90298-7;
RA   Kurjan J., Herskowitz I.;
RT   "Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor
RT   precursor contains four tandem copies of mature alpha-factor.";
RL   Cell 30:933-943(1982).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Italicus / ATCC 2367 / CBS 423 / IFO 0253 / NRRL Y-1536;
RX   PubMed=2834101; DOI=10.1007/bf00365748;
RA   Kitada K., Hishinuma F.;
RT   "Evidence for preferential multiplication of the internal unit in tandem
RT   repeats of the mating factor alpha genes in Saccharomyces yeasts.";
RL   Curr. Genet. 13:1-5(1988).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169875;
RA   Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA   Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA   Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA   Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA   DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA   Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA   Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA   Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA   Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA   Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA   Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA   Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA   Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA   Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA   Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA   Vo D.H., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL   Nature 387:103-105(1997).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-9.
RX   PubMed=2959859; DOI=10.1128/mcb.7.9.3185;
RA   Inokuchi K., Nakayama A., Hishinuma F.;
RT   "Identification of sequence elements that confer cell-type-specific control
RT   of MF alpha 1 expression in Saccharomyces cerevisiae.";
RL   Mol. Cell. Biol. 7:3185-3193(1987).
RN   [7]
RP   PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RA   Stoetzler D., Kiltz H.-H., Duntze W.;
RT   "Primary structure of alpha-factor peptides from Saccharomyces
RT   cerevisiae.";
RL   Eur. J. Biochem. 69:397-400(1976).
RN   [8]
RP   PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RX   PubMed=340452; DOI=10.1093/oxfordjournals.jbchem.a131864;
RA   Tanaka T., Kita H., Murakami T., Narita K.;
RT   "Purification and amino acid sequence of mating factor from Saccharomyces
RT   cerevisiae.";
RL   J. Biochem. 82:1681-1687(1977).
CC   -!- FUNCTION: The active factor is excreted into the culture medium by
CC       haploid cells of the alpha mating type and acts on cells of the
CC       opposite mating type (type A). It mediates the conjugation process
CC       between the two types by inhibiting the initiation of DNA synthesis in
CC       type a cells and synchronizing them with type alpha.
CC   -!- DOMAIN: The mating factor alpha-1 precursor is identical in S.italicus,
CC       S.uvarum and S.cerevisiae, except for the number of tandem repeat
CC       units: 5, 3 and 4 respectively.
DR   EMBL; X01581; CAA25734.1; -; Genomic_DNA.
DR   EMBL; X01581; CAA25735.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X01581; CAA25736.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X01581; CAA25737.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X01581; CAA25738.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; J01340; AAA88727.1; -; Genomic_DNA.
DR   EMBL; M55016; AAA34778.1; -; Genomic_DNA.
DR   EMBL; Z73543; CAA97899.1; -; Genomic_DNA.
DR   EMBL; M17301; AAA34777.1; -; Genomic_DNA.
DR   EMBL; BK006949; DAA11248.1; -; Genomic_DNA.
DR   PIR; S65199; JFBY1.
DR   RefSeq; NP_015137.1; NM_001184001.1.
DR   BioGRID; 35996; 17.
DR   DIP; DIP-2393N; -.
DR   IntAct; P01149; 2.
DR   STRING; 4932.YPL187W; -.
DR   PaxDb; P01149; -.
DR   PRIDE; P01149; -.
DR   EnsemblFungi; YPL187W_mRNA; YPL187W; YPL187W.
DR   GeneID; 855914; -.
DR   KEGG; sce:YPL187W; -.
DR   EuPathDB; FungiDB:YPL187W; -.
DR   SGD; S000006108; MF(ALPHA)1.
DR   eggNOG; ENOG502S0T3; Eukaryota.
DR   GeneTree; ENSGT00940000176792; -.
DR   HOGENOM; CLU_136956_0_0_1; -.
DR   InParanoid; P01149; -.
DR   KO; K11234; -.
DR   OMA; AWHWLEL; -.
DR   BioCyc; YEAST:G3O-34080-MONOMER; -.
DR   PRO; PR:P01149; -.
DR   Proteomes; UP000002311; Chromosome XVI.
DR   RNAct; P01149; protein.
DR   GO; GO:0005576; C:extracellular region; IDA:SGD.
DR   GO; GO:1903135; F:cupric ion binding; IDA:SGD.
DR   GO; GO:0000772; F:mating pheromone activity; IDA:SGD.
DR   GO; GO:0007618; P:mating; IEA:InterPro.
DR   GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
DR   InterPro; IPR006742; Mating_factor_alpha_C.
DR   InterPro; IPR008675; Mating_factor_alpha_N.
DR   Pfam; PF04648; MF_alpha; 4.
DR   Pfam; PF05436; MF_alpha_N; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Pheromone;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..19
FT                   /note="Or 20"
FT                   /evidence="ECO:0000305|PubMed:340452, ECO:0000305|Ref.7"
FT   PROPEP          20..89
FT                   /id="PRO_0000419673"
FT   PEPTIDE         90..102
FT                   /note="Mating factor alpha"
FT                   /id="PRO_0000021697"
FT   PROPEP          105..110
FT                   /id="PRO_0000419674"
FT   PEPTIDE         111..123
FT                   /note="Mating factor alpha"
FT                   /id="PRO_0000021698"
FT   PROPEP          126..131
FT                   /id="PRO_0000419675"
FT   PEPTIDE         132..144
FT                   /note="Mating factor alpha"
FT                   /id="PRO_0000021699"
FT   PROPEP          147..152
FT                   /id="PRO_0000419676"
FT   PEPTIDE         153..165
FT                   /note="Mating factor alpha"
FT                   /id="PRO_0000021700"
FT   VARIANT         165
FT                   /note="Y -> YKREADAEAWHWLQLKPGQPMY (in strain: Italicus /
FT                   IFO 0253)"
FT   CONFLICT        42
FT                   /note="L -> S (in Ref. 2; AAA88727)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        107
FT                   /note="E -> D (in Ref. 3; AAA34778)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   165 AA;  18642 MW;  826ACB932DE3D128 CRC64;
     MRFPSIFTAV LFAASSALAA PVNTTTEDET AQIPAEAVIG YLDLEGDFDV AVLPFSNSTN
     NGLLFINTTI ASIAAKEEGV SLDKREAEAW HWLQLKPGQP MYKREAEAEA WHWLQLKPGQ
     PMYKREADAE AWHWLQLKPG QPMYKREADA EAWHWLQLKP GQPMY
//
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