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Database: UniProt
Entry: MFAL2_YEAST
LinkDB: MFAL2_YEAST
Original site: MFAL2_YEAST 
ID   MFAL2_YEAST             Reviewed;         120 AA.
AC   P32435; D6VU56;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   12-AUG-2020, entry version 125.
DE   RecName: Full=Mating factor alpha-2;
DE   AltName: Full=Alpha-2 mating pheromone;
DE   Contains:
DE     RecName: Full=Mating factor alpha;
DE   Contains:
DE     RecName: Full=Mating factor alpha-like;
DE   Flags: Precursor;
GN   Name=MF(ALPHA)2; Synonyms=MF-ALPHA-2, MFAL2; OrderedLocusNames=YGL089C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=6306574; DOI=10.1093/nar/11.12.4049;
RA   Singh A., Chen E.Y., Lugovoy J.M., Chang C.N., Hitzeman R.A., Seeburg P.H.;
RT   "Saccharomyces cerevisiae contains two discrete genes coding for the alpha-
RT   factor pheromone.";
RL   Nucleic Acids Res. 11:4049-4063(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9290212;
RX   DOI=10.1002/(sici)1097-0061(19970915)13:11<1077::aid-yea152>3.0.co;2-y;
RA   Rieger M., Brueckner M., Schaefer M., Mueller-Auer S.;
RT   "Sequence analysis of 203 kilobases from Saccharomyces cerevisiae
RT   chromosome VII.";
RL   Yeast 13:1077-1090(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RA   Stoetzler D., Kiltz H.-H., Duntze W.;
RT   "Primary structure of alpha-factor peptides from Saccharomyces
RT   cerevisiae.";
RL   Eur. J. Biochem. 69:397-400(1976).
RN   [6]
RP   PROTEIN SEQUENCE OF THE ACTIVE FACTOR.
RX   PubMed=340452; DOI=10.1093/oxfordjournals.jbchem.a131864;
RA   Tanaka T., Kita H., Murakami T., Narita K.;
RT   "Purification and amino acid sequence of mating factor from Saccharomyces
RT   cerevisiae.";
RL   J. Biochem. 82:1681-1687(1977).
CC   -!- FUNCTION: The active factor is excreted into the culture medium by
CC       haploid cells of the alpha mating type and acts on cells of the
CC       opposite mating type (type A). It mediates the conjugation process
CC       between the two types by inhibiting the initiation of DNA synthesis in
CC       type a cells and synchronizing them with type alpha.
DR   EMBL; X01582; CAA25739.1; -; Genomic_DNA.
DR   EMBL; X01582; CAA25740.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X01582; CAA25741.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; Z72611; CAA96795.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08017.1; -; Genomic_DNA.
DR   PIR; S05791; S05791.
DR   RefSeq; NP_011426.1; NM_001180954.1.
DR   BioGRID; 33162; 14.
DR   STRING; 4932.YGL089C; -.
DR   iPTMnet; P32435; -.
DR   PaxDb; P32435; -.
DR   EnsemblFungi; YGL089C_mRNA; YGL089C; YGL089C.
DR   GeneID; 852791; -.
DR   KEGG; sce:YGL089C; -.
DR   EuPathDB; FungiDB:YGL089C; -.
DR   SGD; S000003057; MF(ALPHA)2.
DR   eggNOG; ENOG502S0T3; Eukaryota.
DR   GeneTree; ENSGT00940000176792; -.
DR   HOGENOM; CLU_136956_0_0_1; -.
DR   InParanoid; P32435; -.
DR   KO; K11234; -.
DR   BioCyc; YEAST:G3O-30589-MONOMER; -.
DR   PRO; PR:P32435; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; P32435; protein.
DR   GO; GO:0005576; C:extracellular region; TAS:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:1903135; F:cupric ion binding; IDA:SGD.
DR   GO; GO:0000772; F:mating pheromone activity; IDA:SGD.
DR   GO; GO:0007618; P:mating; IEA:InterPro.
DR   GO; GO:0000750; P:pheromone-dependent signal transduction involved in conjugation with cellular fusion; IMP:SGD.
DR   InterPro; IPR016326; Mating_factor_a.
DR   InterPro; IPR006742; Mating_factor_alpha_C.
DR   InterPro; IPR008675; Mating_factor_alpha_N.
DR   Pfam; PF04648; MF_alpha; 2.
DR   Pfam; PF05436; MF_alpha_N; 1.
DR   PIRSF; PIRSF001866; Mating_factor_alpha; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing; Pheromone;
KW   Reference proteome; Repeat; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   PROPEP          22..86
FT                   /id="PRO_0000419677"
FT   PEPTIDE         87..99
FT                   /note="Mating factor alpha-like"
FT                   /id="PRO_0000021702"
FT   PROPEP          102..107
FT                   /id="PRO_0000419678"
FT   PEPTIDE         108..120
FT                   /note="Mating factor alpha"
FT                   /id="PRO_0000021703"
SQ   SEQUENCE   120 AA;  13271 MW;  10BF3FDB985FBB2D CRC64;
     MKFISTFLTF ILAAVSVTAS SDEDIAQVPA EAIIGYLDFG GDHDIAFLPF SNATASGLLF
     INTTIAEAAE KEQNTTLAKR EAVADAWHWL NLRPGQPMYK REANADAWHW LQLKPGQPMY
//
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