ID MON1B_HUMAN Reviewed; 547 AA.
AC Q7L1V2; B4DDZ0; O94949;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 27-MAR-2024, entry version 146.
DE RecName: Full=Vacuolar fusion protein MON1 homolog B;
DE AltName: Full=HSV-1 stimulation-related gene 1 protein;
DE AltName: Full=HSV-I stimulating-related protein;
GN Name=MON1B; Synonyms=HSRG1, KIAA0872, SAND2;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND INDUCTION BY HSV-1.
RC TISSUE=Lung;
RX PubMed=12828340;
RA Dong S.Z., Dong C.H., Liu L.D., Li Q.H.;
RT "Identification of a novel human MON1/SAND family protein in human
RT fibroblasts induced by herpes simplex virus 1 binding.";
RL Acta Virol. 47:27-32(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=10048485; DOI=10.1093/dnares/5.6.355;
RA Nagase T., Ishikawa K., Suyama M., Kikuno R., Hirosawa M., Miyajima N.,
RA Tanaka A., Kotani H., Nomura N., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XII. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 5:355-364(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Urinary bladder;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15616553; DOI=10.1038/nature03187;
RA Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G.,
RA Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E.,
RA Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J., Buckingham J.M.,
RA Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C.,
RA Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M.,
RA Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M.,
RA Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D.,
RA Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L.,
RA Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E.,
RA Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H.,
RA Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y.,
RA Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J.,
RA Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D.,
RA Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S.,
RA Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A.,
RA Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M.,
RA Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H.,
RA Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A.,
RA Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J.,
RA DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J.,
RA Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M.,
RA Myers R.M., Rubin E.M., Pennacchio L.A.;
RT "The sequence and analysis of duplication-rich human chromosome 16.";
RL Nature 432:988-994(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP INTERACTION WITH CCNT2.
RX PubMed=21509660; DOI=10.1007/s11427-011-4160-3;
RA Wu W., Yu X., Li W., Guo L., Liu L., Wang L., Li Q.;
RT "HSV-1 stimulation-related protein HSRG1 inhibits viral gene
RT transcriptional elongation by interacting with Cyclin T2.";
RL Sci. China Life Sci. 54:359-365(2011).
RN [9]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59 AND SER-61, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma, and Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
RN [11]
RP IDENTIFICATION IN A COMPLEX RMC1; CCZ1; MON1A AND MON1B.
RX PubMed=29038162; DOI=10.1128/mcb.00392-17;
RA Pontano Vaites L., Paulo J.A., Huttlin E.L., Harper J.W.;
RT "Systematic analysis of human cells lacking ATG8 proteins uncovers roles
RT for GABARAPs and the CCZ1/MON1 regulator C18orf8/RMC1 in macro and
RT selective autophagic flux.";
RL Mol. Cell. Biol. 0:0-0(2017).
CC -!- SUBUNIT: Interacts with CCNT2; down-regulates CCNT2-mediated activation
CC of viral promoters during herpes simplex virus 1/HHV-1 infection
CC (PubMed:21509660). Found in a complex with RMC1, CCZ1 MON1A and MON1B
CC (PubMed:29038162). {ECO:0000269|PubMed:21509660,
CC ECO:0000269|PubMed:29038162}.
CC -!- INTERACTION:
CC Q7L1V2; Q9P253: VPS18; NbExp=2; IntAct=EBI-2655311, EBI-1053363;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q7L1V2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7L1V2-2; Sequence=VSP_054664, VSP_054665;
CC -!- INDUCTION: Induced in fibroblast KMB17 cells by HSV-1.
CC {ECO:0000269|PubMed:12828340}.
CC -!- SIMILARITY: Belongs to the MON1/SAND family. {ECO:0000305}.
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DR EMBL; AF442486; AAL35292.1; -; mRNA.
DR EMBL; AB020679; BAA74895.2; -; mRNA.
DR EMBL; AK293392; BAG56901.1; -; mRNA.
DR EMBL; AC009139; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC024277; AAH24277.1; -; mRNA.
DR CCDS; CCDS10925.1; -. [Q7L1V2-1]
DR CCDS; CCDS67082.1; -. [Q7L1V2-2]
DR RefSeq; NP_001273568.1; NM_001286639.1.
DR RefSeq; NP_001273569.1; NM_001286640.1. [Q7L1V2-2]
DR RefSeq; NP_055755.1; NM_014940.3. [Q7L1V2-1]
DR AlphaFoldDB; Q7L1V2; -.
DR SMR; Q7L1V2; -.
DR BioGRID; 116546; 20.
DR ComplexPortal; CPX-8152; MON1-CCZ1 guanyl-nucleotide exchange factor complex, MON1B variant.
DR ComplexPortal; CPX-8166; MON1-CCZ1B guanyl-nucleotide exchange factor complex, MON1B variant.
DR IntAct; Q7L1V2; 13.
DR STRING; 9606.ENSP00000248248; -.
DR iPTMnet; Q7L1V2; -.
DR PhosphoSitePlus; Q7L1V2; -.
DR BioMuta; MON1B; -.
DR DMDM; 74738536; -.
DR EPD; Q7L1V2; -.
DR jPOST; Q7L1V2; -.
DR MassIVE; Q7L1V2; -.
DR MaxQB; Q7L1V2; -.
DR PaxDb; 9606-ENSP00000248248; -.
DR PeptideAtlas; Q7L1V2; -.
DR ProteomicsDB; 3908; -.
DR ProteomicsDB; 68751; -. [Q7L1V2-1]
DR Pumba; Q7L1V2; -.
DR TopDownProteomics; Q7L1V2-1; -. [Q7L1V2-1]
DR Antibodypedia; 30376; 121 antibodies from 20 providers.
DR DNASU; 22879; -.
DR Ensembl; ENST00000248248.8; ENSP00000248248.3; ENSG00000103111.15. [Q7L1V2-1]
DR Ensembl; ENST00000545553.1; ENSP00000444881.1; ENSG00000103111.15. [Q7L1V2-2]
DR GeneID; 22879; -.
DR KEGG; hsa:22879; -.
DR MANE-Select; ENST00000248248.8; ENSP00000248248.3; NM_014940.4; NP_055755.1.
DR UCSC; uc002fez.5; human. [Q7L1V2-1]
DR AGR; HGNC:25020; -.
DR CTD; 22879; -.
DR DisGeNET; 22879; -.
DR GeneCards; MON1B; -.
DR HGNC; HGNC:25020; MON1B.
DR HPA; ENSG00000103111; Low tissue specificity.
DR MIM; 608954; gene.
DR neXtProt; NX_Q7L1V2; -.
DR OpenTargets; ENSG00000103111; -.
DR PharmGKB; PA142671341; -.
DR VEuPathDB; HostDB:ENSG00000103111; -.
DR eggNOG; KOG0997; Eukaryota.
DR GeneTree; ENSGT00390000006665; -.
DR HOGENOM; CLU_014574_4_1_1; -.
DR InParanoid; Q7L1V2; -.
DR OMA; LEPTDLH; -.
DR OrthoDB; 73361at2759; -.
DR PhylomeDB; Q7L1V2; -.
DR PathwayCommons; Q7L1V2; -.
DR Reactome; R-HSA-8876198; RAB GEFs exchange GTP for GDP on RABs.
DR SignaLink; Q7L1V2; -.
DR BioGRID-ORCS; 22879; 16 hits in 1147 CRISPR screens.
DR ChiTaRS; MON1B; human.
DR GenomeRNAi; 22879; -.
DR Pharos; Q7L1V2; Tbio.
DR PRO; PR:Q7L1V2; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q7L1V2; Protein.
DR Bgee; ENSG00000103111; Expressed in palpebral conjunctiva and 188 other cell types or tissues.
DR ExpressionAtlas; Q7L1V2; baseline and differential.
DR Genevisible; Q7L1V2; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0035658; C:Mon1-Ccz1 complex; IDA:UniProtKB.
DR GO; GO:0019085; P:early viral transcription; IDA:UniProtKB.
DR GO; GO:0019086; P:late viral transcription; IDA:UniProtKB.
DR GO; GO:0006623; P:protein targeting to vacuole; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR043972; FUZ/MON1/HPS1_longin_1.
DR InterPro; IPR043971; FUZ/MON1/HPS1_longin_2.
DR InterPro; IPR043970; FUZ/MON1/HPS1_longin_3.
DR InterPro; IPR004353; Mon1.
DR PANTHER; PTHR13027; SAND PROTEIN-RELATED; 1.
DR PANTHER; PTHR13027:SF13; VACUOLAR FUSION PROTEIN MON1 HOMOLOG B; 1.
DR Pfam; PF19036; Fuz_longin_1; 1.
DR Pfam; PF19037; Fuz_longin_2; 1.
DR Pfam; PF19038; Fuz_longin_3; 1.
DR PRINTS; PR01546; YEAST73DUF.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..547
FT /note="Vacuolar fusion protein MON1 homolog B"
FT /id="PRO_0000285765"
FT REGION 1..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..54
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..98
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22814378"
FT MOD_RES 59
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT MOD_RES 61
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..12
FT /note="MEVGGDTAAPAP -> MVSGQLRFGVKT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054664"
FT VAR_SEQ 13..158
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_054665"
SQ SEQUENCE 547 AA; 59217 MW; 06ED0B7C42AA2D17 CRC64;
MEVGGDTAAP APGGAEDLED TQFPSEEARE GGGVHAVPPD PEDEGLEETG SKDKDQPPSP
SPPPQSEALS STSRLWSPAA PENSPTCSPE SSSGGQGGDP SDEEWRSQRK HVFVLSEAGK
PIYSRYGSVE ALSATMGVMT ALVSFVQSAG DAIRAIYAED HKLVFLQQGP LLLVAMSRTS
QSAAQLRGEL LAVHAQIVST LTRASVARIF AHKQNYDLRR LLAGSERTLD RLLDSMEQDP
GALLLGAVRC VPLARPLRDA LGALLRRCTA PGLALSVLAV GGRLITAAQE RNVLAECRLD
PADLQLLLDW VGAPAFAAGE AWAPVCLPRF NPDGFFYAYV ARLDAMPVCL LLLGTQREAF
HAMAACRRLV EDGMHALGAM RALGEAASFS NASSASAPAY SVQAVGAPGL RHFLYKPLDI
PDHHRQLPQF TSPELEAPYS REEERQRLSD LYHRLHARLH STSRPLRLIY HVAEKETLLA
WVTSKFELYT CLSPLVTKAG AILVVTKLLR WVKKEEDRLF IRYPPKYSTP PATSTDQAAH
NGLFTGL
//
