ID MTDTR_PSEAE Reviewed; 110 AA.
AC Q9HUH5;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=Multidrug transporter PA4990;
GN OrderedLocusNames=PA4990;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBCELLULAR LOCATION.
RX PubMed=11574548; DOI=10.1074/jbc.m108231200;
RA Ninio S., Rotem D., Schuldiner S.;
RT "Functional analysis of novel multidrug transporters from human
RT pathogens.";
RL J. Biol. Chem. 276:48250-48256(2001).
CC -!- FUNCTION: Confers resistance to ethidium bromide, acriflavine and
CC methyl viologen. {ECO:0000269|PubMed:11574548}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=2426 uM for methyl viologen {ECO:0000269|PubMed:11574548};
CC Vmax=4800 nmol/min/mg enzyme with methyl viologen as substrate
CC {ECO:0000269|PubMed:11574548};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:11574548};
CC Multi-pass membrane protein {ECO:0000269|PubMed:11574548}.
CC -!- SIMILARITY: Belongs to the drug/metabolite transporter (DMT)
CC superfamily. Small multidrug resistance (SMR) (TC 2.A.7.1) family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG08375.1; -; Genomic_DNA.
DR PIR; C83021; C83021.
DR RefSeq; NP_253677.1; NC_002516.2.
DR RefSeq; WP_003095735.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HUH5; -.
DR SMR; Q9HUH5; -.
DR STRING; 208964.PA4990; -.
DR PaxDb; 208964-PA4990; -.
DR DNASU; 880147; -.
DR GeneID; 880147; -.
DR KEGG; pae:PA4990; -.
DR PATRIC; fig|208964.12.peg.5230; -.
DR PseudoCAP; PA4990; -.
DR HOGENOM; CLU_133067_0_2_6; -.
DR InParanoid; Q9HUH5; -.
DR OrthoDB; 9808638at2; -.
DR PhylomeDB; Q9HUH5; -.
DR BioCyc; PAER208964:G1FZ6-5106-MONOMER; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015199; F:amino-acid betaine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015297; F:antiporter activity; IBA:GO_Central.
DR GO; GO:0015220; F:choline transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015871; P:choline transport; IBA:GO_Central.
DR GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR GO; GO:0042221; P:response to chemical; IEA:UniProt.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.10.3730.20; -; 1.
DR InterPro; IPR000390; Small_drug/metabolite_transptr.
DR InterPro; IPR045324; Small_multidrug_res.
DR PANTHER; PTHR30561:SF1; MULTIDRUG TRANSPORTER EMRE; 1.
DR PANTHER; PTHR30561; SMR FAMILY PROTON-DEPENDENT DRUG EFFLUX TRANSPORTER SUGE; 1.
DR Pfam; PF00893; Multi_Drug_Res; 1.
DR SUPFAM; SSF103481; Multidrug resistance efflux transporter EmrE; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..110
FT /note="Multidrug transporter PA4990"
FT /id="PRO_0000425956"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 110 AA; 11489 MW; 95ED027B31DF52FE CRC64;
MTNYLYLAIA IAAEVVATTS LKAVAGFSKP LPLLLVVGGY VLAFSMLVLV MRTLPVGVVY
AIWSGLGIVL VSLVAMFVYG QRLDPAALLG IGLIIAGVLV IQLFSRASGH
//
