ID MUB2_ARATH Reviewed; 124 AA.
AC Q8LCS8; Q9LF36;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 24-JAN-2024, entry version 114.
DE RecName: Full=Membrane-anchored ubiquitin-fold protein 2;
DE Short=AtMUB2;
DE Short=Membrane-anchored ub-fold protein 2;
DE AltName: Full=NTGP5;
GN Name=MUB2; OrderedLocusNames=At5g15460; ORFNames=T20K14.70;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION, NOMENCLATURE, ISOPRENYLATION, PALMITOYLATION AT CYS-115;
RP CYS-117; CYS-119 AND CYS-124, MUTAGENESIS OF CYS-115; CYS-117; CYS-119;
RP CYS-124 AND 115-CYS--CYS-124, TISSUE SPECIFICITY, INDUCTION, AND
RP SUBCELLULAR LOCATION.
RX PubMed=16831869; DOI=10.1074/jbc.m602283200;
RA Downes B.P., Saracco S.A., Lee S.S., Crowell D.N., Vierstra R.D.;
RT "MUBS: a family of ubiquitin-fold proteins that are plasma membrane-
RT anchored by prenylation.";
RL J. Biol. Chem. 281:27145-27157(2006).
CC -!- FUNCTION: May serve as docking site to facilitate the association of
CC other proteins to the plasma membrane.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:16831869};
CC Lipid-anchor {ECO:0000269|PubMed:16831869}.
CC -!- TISSUE SPECIFICITY: Ubiquitous, but three fold higher expression in
CC stamens. {ECO:0000269|PubMed:16831869}.
CC -!- INDUCTION: Not induced by pathogens, cycloheximide and ozone treatment.
CC {ECO:0000269|PubMed:16831869}.
CC -!- PTM: Acylated protein. Probably modified with palmitate
CC (PubMed:16831869). {ECO:0000269|PubMed:16831869}.
CC -!- MISCELLANEOUS: Heat stable and remains soluble at temperatures
CC exceeding 90 degrees Celsius.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC01745.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL391143; CAC01745.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92163.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92164.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69826.1; -; Genomic_DNA.
DR EMBL; BT006108; AAP04093.1; -; mRNA.
DR EMBL; BT005773; AAO64177.1; -; mRNA.
DR EMBL; AY086424; AAM63426.1; -; mRNA.
DR EMBL; AK228599; BAF00514.1; -; mRNA.
DR PIR; T51524; T51524.
DR RefSeq; NP_001318568.1; NM_001343401.1.
DR RefSeq; NP_568315.1; NM_121550.3.
DR RefSeq; NP_850824.1; NM_180493.4.
DR AlphaFoldDB; Q8LCS8; -.
DR SMR; Q8LCS8; -.
DR BioGRID; 16675; 4.
DR STRING; 3702.Q8LCS8; -.
DR PaxDb; 3702-AT5G15460-2; -.
DR ProteomicsDB; 250741; -.
DR EnsemblPlants; AT5G15460.1; AT5G15460.1; AT5G15460.
DR EnsemblPlants; AT5G15460.2; AT5G15460.2; AT5G15460.
DR EnsemblPlants; AT5G15460.4; AT5G15460.4; AT5G15460.
DR GeneID; 831399; -.
DR Gramene; AT5G15460.1; AT5G15460.1; AT5G15460.
DR Gramene; AT5G15460.2; AT5G15460.2; AT5G15460.
DR Gramene; AT5G15460.4; AT5G15460.4; AT5G15460.
DR KEGG; ath:AT5G15460; -.
DR Araport; AT5G15460; -.
DR TAIR; AT5G15460; MUB2.
DR eggNOG; ENOG502RZYK; Eukaryota.
DR HOGENOM; CLU_136465_1_0_1; -.
DR InParanoid; Q8LCS8; -.
DR OMA; CRSPICD; -.
DR OrthoDB; 312659at2759; -.
DR PhylomeDB; Q8LCS8; -.
DR PRO; PR:Q8LCS8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8LCS8; baseline and differential.
DR Genevisible; Q8LCS8; AT.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR CDD; cd01814; Ubl_MUBs_plant; 1.
DR InterPro; IPR017000; MUB.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR040015; UBL3-like.
DR InterPro; IPR039540; UBL3-like_ubiquitin_dom.
DR PANTHER; PTHR13169:SF26; MEMBRANE-ANCHORED UBIQUITIN-FOLD PROTEIN 2; 1.
DR PANTHER; PTHR13169; UBIQUITIN-LIKE PROTEIN 3 HCG-1 PROTEIN; 1.
DR Pfam; PF13881; Rad60-SLD_2; 1.
DR PIRSF; PIRSF032572; MUB; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Reference proteome.
FT CHAIN 1..124
FT /note="Membrane-anchored ubiquitin-fold protein 2"
FT /id="PRO_0000248164"
FT DOMAIN 8..74
FT /note="Ubiquitin-like"
FT LIPID 115
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 117
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 119
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000305|PubMed:16831869"
FT LIPID 124
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000305|PubMed:16831869"
FT MUTAGEN 115..124
FT /note="CVCLCFGARC->SVSLSFGARS: Loss of membrane
FT localization."
FT /evidence="ECO:0000269|PubMed:16831869"
FT MUTAGEN 115
FT /note="C->S: No effect on localization."
FT /evidence="ECO:0000269|PubMed:16831869"
FT MUTAGEN 117
FT /note="C->S: No effect on localization."
FT /evidence="ECO:0000269|PubMed:16831869"
FT MUTAGEN 119
FT /note="C->S: Loss of membrane localization."
FT /evidence="ECO:0000269|PubMed:16831869"
FT MUTAGEN 124
FT /note="C->S: Loss of membrane localization."
FT /evidence="ECO:0000269|PubMed:16831869"
SQ SEQUENCE 124 AA; 13709 MW; F8F27BE021537DC2 CRC64;
MAEVKDQLEI KFRLNDGSDI GPKLFPDATT VATLKETVVA QWPRDKENGP KTVKDVKLIS
AGRILENNKT VGDCRSPVGN FSGAVTTMHV IIQHQVTEKE KKKKKPKGDL KQNKCVCLCF
GARC
//
