ID MUT7_CAEEL Reviewed; 910 AA.
AC P34607;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 27-MAR-2024, entry version 155.
DE RecName: Full=Exonuclease mut-7;
DE EC=3.1.-.-;
DE AltName: Full=Exonuclease 3'-5' domain-containing protein 3 homolog;
GN Name=mut-7; ORFNames=ZK1098.8;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=7906398; DOI=10.1038/368032a0;
RA Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA Wilkinson-Sproat J., Wohldman P.;
RT "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT elegans.";
RL Nature 368:32-38(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=10535732; DOI=10.1016/s0092-8674(00)81645-1;
RA Ketting R.F., Haverkamp T.H., van Luenen H.G., Plasterk R.H.;
RT "Mut-7 of C. elegans, required for transposon silencing and RNA
RT interference, is a homolog of Werner syndrome helicase and RNaseD.";
RL Cell 99:133-141(1999).
CC -!- FUNCTION: Represses the transposition of Tc1, Tc3, Tc4, and Tc5,
CC perhaps by degrading transposon-specific messages. Also affects sperm
CC development, sensitivity to RNAi of mainly germline expressed genes,
CC silencing of some germline transgenes, X chromosome loss, and is
CC required for cosuppression (functional silencing of chromosomal loci
CC induced by transgenes) and for silencing induced by antisense RNA
CC oligomers. {ECO:0000269|PubMed:10535732}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- INTERACTION:
CC P34607; Q19672: rde-2; NbExp=8; IntAct=EBI-476365, EBI-476355;
CC -!- SIMILARITY: Belongs to the mut-7 family. {ECO:0000305}.
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DR EMBL; Z22176; CAA80137.1; -; Genomic_DNA.
DR PIR; S40930; S40930.
DR RefSeq; NP_499105.1; NM_066704.6.
DR AlphaFoldDB; P34607; -.
DR SMR; P34607; -.
DR BioGRID; 41542; 8.
DR IntAct; P34607; 1.
DR STRING; 6239.ZK1098.8.1; -.
DR EPD; P34607; -.
DR PaxDb; 6239-ZK1098-8; -.
DR PeptideAtlas; P34607; -.
DR EnsemblMetazoa; ZK1098.8.1; ZK1098.8.1; WBGene00003504.
DR GeneID; 176347; -.
DR KEGG; cel:CELE_ZK1098.8; -.
DR UCSC; ZK1098.8; c. elegans.
DR AGR; WB:WBGene00003504; -.
DR WormBase; ZK1098.8; CE00370; WBGene00003504; mut-7.
DR eggNOG; KOG2207; Eukaryota.
DR GeneTree; ENSGT00390000006843; -.
DR HOGENOM; CLU_354213_0_0_1; -.
DR InParanoid; P34607; -.
DR OMA; SSKLRMC; -.
DR OrthoDB; 52347at2759; -.
DR PhylomeDB; P34607; -.
DR PRO; PR:P34607; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00003504; Expressed in germ line (C elegans) and 4 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:WormBase.
DR GO; GO:1990633; C:mutator focus; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IBA:GO_Central.
DR GO; GO:0044748; F:3'-5'-exoribonuclease activity involved in mature miRNA 3'-end processing; ISS:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0008306; P:associative learning; IMP:WormBase.
DR GO; GO:0008355; P:olfactory learning; IMP:WormBase.
DR GO; GO:0016441; P:post-transcriptional gene silencing; IMP:WormBase.
DR GO; GO:0044747; P:pre-miRNA 3'-end processing; ISS:WormBase.
DR GO; GO:0035194; P:regulatory ncRNA-mediated post-transcriptional gene silencing; IMP:WormBase.
DR CDD; cd06146; mut-7_like_exo; 1.
DR CDD; cd18772; PIN_Mut7-C-like; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR037432; Mut-7_DEDDy_dom.
DR InterPro; IPR002782; Mut7-C_RNAse_dom.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR47765; 3'-5' EXONUCLEASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR47765:SF2; EXONUCLEASE MUT-7 HOMOLOG; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR Pfam; PF01927; Mut7-C; 1.
DR SMART; SM00474; 35EXOc; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 1: Evidence at protein level;
KW Exonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome; RNA-mediated gene silencing.
FT CHAIN 1..910
FT /note="Exonuclease mut-7"
FT /id="PRO_0000096650"
FT DOMAIN 404..605
FT /note="3'-5' exonuclease"
SQ SEQUENCE 910 AA; 105569 MW; 5512D15423517FCD CRC64;
MEEEPYKRKL TKAEKKAKYR TDYAEPLKSR REVLKAIMNG PESERERKVR AKNREFFNED
YRSGVNIYGM AVDMMKAMPD RGKTSGQSLA VWYLEDFGVW LKESGQETEL RQKYLTGTIQ
INALDVCTIG QKQLLSEIFD ITKEKFTEDI TQLLDAAIKK QDFSVAADMA IQYNLLRDHH
FEHLVLPLML SGKDQTAYKL ISNNERMQQQ LVEFFDRMVG ISVVAVEEML KPYKETKIMT
IPMEKLTGKT LDKLISTIIN KNTHEYNFSR ELSKFAKNHS QNGNLKALKF NISERYEKGK
SDDNYFQHMV ETFTKAEDVR EPILFYLWSS NDTEKQIDAI CFAIYLGIAS SSSYQLPNVM
RDFFRQPDSK LREAKELLVR RKTLQVPLNG EQLFVFENER RTQIHMVKTE SEMNYLCSEI
KSLSDEPAPV YVGFDSEWKP SNLTAVHDSK IAIIQLFFKN CVWLVDCVEL EKANMADDWW
QKFASRLFGD SPVKVVGFDM RNDLDAMATI PALKSSMKIE DTKNAFDLKR LAENVCDIDM
EILELPKKTF KLADLTHYLL GLELDKTEQC SNWQCRPLRK KQIVYAALDA VVVVETFKKI
LSIVEEKNKD ADIEKIVRES NVMAPKKDKG HKSYRKLKTI PWLELYDILR SHRNPTRSPQ
RPHDIKVIVD TMLIGFGKNL RRVGIDVILP KDVSDFRKYL KEIERVGGEH LRHIITVPSK
SYEALKMDYD NYTIAIPELN NMSPVDQLIE FFDLFNVDIR PEDVYPRCTE CNSRLQIKFP
GPVLHFLHQY CVIHVQNVYR ADMSEFPLEE WWNRMLHINP DDYDGVKVEM SRPSPTSKWI
VATVPTGCLH ITRQTALHTN LPDGIEVRIH KVPDDEFKRR NLSFYVCGEC GTVACDGRGN
QASESTSQEC
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