ID MYO4_ARATH Reviewed; 1134 AA.
AC F4JIU4; O81840; Q9M0J7;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 24-JAN-2024, entry version 72.
DE RecName: Full=Myosin-4;
DE AltName: Full=MYOSIN VIII B;
DE Short=AtVIIIB;
GN Name=VIII-B; Synonyms=VIIIB; OrderedLocusNames=At4g27370;
GN ORFNames=M4I22.180;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=10984423; DOI=10.1242/jcs.113.19.3353;
RA Hodge T., Cope M.J.;
RT "A myosin family tree.";
RL J. Cell Sci. 113:3353-3354(2000).
RN [4]
RP GENE FAMILY.
RX PubMed=11516337; DOI=10.1186/gb-2001-2-7-research0024;
RA Reddy A.S., Day I.S.;
RT "Analysis of the myosins encoded in the recently completed Arabidopsis
RT thaliana genome sequence.";
RL Genome Biol. 2:RESEARCH0024.1-RESEARCH0024.17(2001).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=21233331; DOI=10.1104/pp.110.170720;
RA Peremyslov V.V., Mockler T.C., Filichkin S.A., Fox S.E., Jaiswal P.,
RA Makarova K.S., Koonin E.V., Dolja V.V.;
RT "Expression, splicing, and evolution of the myosin gene family in plants.";
RL Plant Physiol. 155:1191-1204(2011).
CC -!- FUNCTION: Myosin heavy chain that is required for the cell cycle-
CC regulated transport of various organelles and proteins for their
CC segregation. Functions by binding with its tail domain to receptor
CC proteins on organelles and exerting force with its N-terminal motor
CC domain against actin filaments, thereby transporting its cargo along
CC polarized actin cables (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- DOMAIN: IQ domain mediates interaction with calmodulin. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC superfamily. Myosin family. Plant myosin class VIII subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA19731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB81387.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL030978; CAA19731.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161571; CAB81387.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85329.1; -; Genomic_DNA.
DR PIR; A85318; A85318.
DR PIR; T05761; T05761.
DR RefSeq; NP_194467.5; NM_118871.6.
DR AlphaFoldDB; F4JIU4; -.
DR SMR; F4JIU4; -.
DR STRING; 3702.F4JIU4; -.
DR PaxDb; 3702-AT4G27370-1; -.
DR EnsemblPlants; AT4G27370.1; AT4G27370.1; AT4G27370.
DR GeneID; 828845; -.
DR Gramene; AT4G27370.1; AT4G27370.1; AT4G27370.
DR KEGG; ath:AT4G27370; -.
DR Araport; AT4G27370; -.
DR TAIR; AT4G27370; VIIIB.
DR eggNOG; KOG0160; Eukaryota.
DR HOGENOM; CLU_000192_7_2_1; -.
DR InParanoid; F4JIU4; -.
DR PRO; PR:F4JIU4; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; F4JIU4; baseline and differential.
DR Genevisible; F4JIU4; AT.
DR GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005516; F:calmodulin binding; IEA:UniProtKB-KW.
DR GO; GO:0003774; F:cytoskeletal motor activity; ISS:TAIR.
DR GO; GO:0030048; P:actin filament-based movement; TAS:TAIR.
DR CDD; cd01383; MYSc_Myo8; 1.
DR Gene3D; 1.10.10.820; -; 1.
DR Gene3D; 1.20.5.190; -; 1.
DR Gene3D; 1.20.58.530; -; 1.
DR Gene3D; 6.20.240.20; -; 1.
DR Gene3D; 3.40.850.10; Kinesin motor domain; 1.
DR Gene3D; 1.20.120.720; Myosin VI head, motor domain, U50 subdomain; 1.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR036961; Kinesin_motor_dom_sf.
DR InterPro; IPR001609; Myosin_head_motor_dom.
DR InterPro; IPR004009; Myosin_N.
DR InterPro; IPR036022; MYSc_Myo8.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR13140; MYOSIN; 1.
DR PANTHER; PTHR13140:SF763; MYOSIN-4; 1.
DR Pfam; PF00612; IQ; 3.
DR Pfam; PF00063; Myosin_head; 1.
DR PRINTS; PR00193; MYOSINHEAVY.
DR SMART; SM00015; IQ; 3.
DR SMART; SM00242; MYSc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50096; IQ; 3.
DR PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR PROSITE; PS51844; SH3_LIKE; 1.
PE 3: Inferred from homology;
KW Actin-binding; ATP-binding; Calmodulin-binding; Coiled coil; Motor protein;
KW Myosin; Nucleotide-binding; Reference proteome; Repeat.
FT CHAIN 1..1134
FT /note="Myosin-4"
FT /id="PRO_0000422860"
FT DOMAIN 110..160
FT /note="Myosin N-terminal SH3-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01190"
FT DOMAIN 164..830
FT /note="Myosin motor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT DOMAIN 832..861
FT /note="IQ 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 855..884
FT /note="IQ 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 891..920
FT /note="IQ 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 589..623
FT /note="Actin-binding"
FT /evidence="ECO:0000255"
FT REGION 710..732
FT /note="Actin-binding"
FT /evidence="ECO:0000250"
FT REGION 913..939
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 953..999
FT /evidence="ECO:0000255"
FT BINDING 255..262
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 304..312
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1134 AA; 129832 MW; 86CFF7F87F88389A CRC64;
MMKSSVKEIL ESLRLLDSSE RSSSLPSPST FRAPMPLIRQ SLPAKFRNAI SLESKTIEKE
DKDWSTEQIT QSAEKEKTGN EVVKISTAQM SRAKNSHDPE WINSAEYFVR EKLCVWCRVA
ANGQWHLGKI HSTSSSDDVC VMLSANDDVR TMEEIFPANP EILEGVEDLT QLSYLNEPSL
LYNLRVRYSQ DLIYSKAGPV LIAVNPFKNV QIYGEEFLSA YQKNALDAPH VYAVADAAYD
DMMREEKNQS IIISGESGAG KTETAKYAMQ YLEALGGGSF GVENEILKTN CILEAFGNAK
TSRNDNSSRF GKLMEIHFSA KGKICGAKLE TFSLDQSRVA QLCNGERCYH IFYQLCAGAS
PILKERLKIK AASEYNYLNQ SNCLTIDRTD DAQKFHKLME AFNIVQIPQE YQERTFALLA
AVLWLGNVSF EVIDNENHVE VVADEAVTNV AMLMGCNSKK LMVVLSTCKL QAGRDCIAKR
LTLRQATDMR DSLAKIIYAS LFNWLVEQIN ISLEVGNSRT GRSISILDIY GFESFKDNSF
EQFCINYANE RLQQHFNRHL FKLEQEEYEG DGIDWTKVEF IDNQECLNLI EKKPIGLVSL
LNEESNFPKA TDTTFANKLK QHLNANSCFK GERGRGFRIK HYAGEVLYNT NGFLEKNRDP
LHVDLIQLLS LCKCQLLNLF STKMHHDFLK PATFSDSMNQ SVIAKFKGQL FKLMNKLEDT
TPHFIRCIKP NSNQLPGLYE ENHVLQQLRC CGVLEIVRIS RSGYPTRLTH QELAVRYGCL
LLDTRISQDP LSTSKAILKQ CNLPPEMYQV GYTKIYLRTG VISVLEERKK YVLRGILGLQ
KQFRGYQTRE YFHNMRNAAV ILQSYIRGEN ARRNYIVVGE SAIVSTAITK ELDAAIHLQY
MVRKWLARKL LNSTQQKNKP RNEKKKTRRK STKRVSEDKE LLSEQFEVQP CVLADLQSRV
LKVEAAIMQK EDENTALQEE LQRFEERWLE NETRMKSMED TWQKHMSSMQ MSLAAACKVL
APDKTASHGT DSEDTMSFGT PTKELKGSLS DVNNLSTEFD QRSVIIHEDP KSLVEVKSDS
ISNRKQHAEE LRRLKSRFEK WKKDYKTRLR ETKARVRLNG DEGRHRNWWC KKSY
//
