ID N1PK43_DOTSN Unreviewed; 459 AA.
AC N1PK43;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Vacuolar calcium ion transporter {ECO:0000256|RuleBase:RU365028};
GN ORFNames=DOTSEDRAFT_129213 {ECO:0000313|EMBL:EME43882.1};
OS Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight
OS fungus) (Mycosphaerella pini).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma.
OX NCBI_TaxID=675120 {ECO:0000313|EMBL:EME43882.1, ECO:0000313|Proteomes:UP000016933};
RN [1] {ECO:0000313|Proteomes:UP000016933}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990 {ECO:0000313|Proteomes:UP000016933};
RX PubMed=23209441; DOI=10.1371/journal.pgen.1003088;
RA de Wit P.J.G.M., van der Burgt A., Oekmen B., Stergiopoulos I.,
RA Abd-Elsalam K.A., Aerts A.L., Bahkali A.H., Beenen H.G., Chettri P.,
RA Cox M.P., Datema E., de Vries R.P., Dhillon B., Ganley A.R.,
RA Griffiths S.A., Guo Y., Hamelin R.C., Henrissat B., Kabir M.S.,
RA Jashni M.K., Kema G., Klaubauf S., Lapidus A., Levasseur A., Lindquist E.,
RA Mehrabi R., Ohm R.A., Owen T.J., Salamov A., Schwelm A., Schijlen E.,
RA Sun H., van den Burg H.A., van Ham R.C.H.J., Zhang S., Goodwin S.B.,
RA Grigoriev I.V., Collemare J., Bradshaw R.E.;
RT "The genomes of the fungal plant pathogens Cladosporium fulvum and
RT Dothistroma septosporum reveal adaptation to different hosts and lifestyles
RT but also signatures of common ancestry.";
RL PLoS Genet. 8:E1003088-E1003088(2012).
RN [2] {ECO:0000313|EMBL:EME43882.1, ECO:0000313|Proteomes:UP000016933}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990 {ECO:0000313|Proteomes:UP000016933};
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
CC -!- FUNCTION: Has a role in promoting intracellular calcium ion
CC sequestration via the exchange of calcium ions for hydrogen ions across
CC the vacuolar membrane. Involved also in manganese ion homeostasis via
CC its uptake into the vacuole. {ECO:0000256|RuleBase:RU365028}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Vacuole membrane
CC {ECO:0000256|RuleBase:RU365028}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000256|ARBA:ARBA00008170, ECO:0000256|RuleBase:RU365028}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|RuleBase:RU365028}.
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DR EMBL; KB446539; EME43882.1; -; Genomic_DNA.
DR AlphaFoldDB; N1PK43; -.
DR STRING; 675120.N1PK43; -.
DR EnsemblFungi; EME43882; EME43882; DOTSEDRAFT_129213.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_1_1_1; -.
DR OMA; EIVMFMV; -.
DR OrthoDB; 276371at2759; -.
DR Proteomes; UP000016933; Unassembled WGS sequence.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1420.30; NCX, central ion-binding region; 2.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR NCBIfam; TIGR00378; cax; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR PANTHER; PTHR31503:SF14; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 3: Inferred from homology;
KW Antiport {ECO:0000256|RuleBase:RU365028};
KW Calcium {ECO:0000256|RuleBase:RU365028};
KW Calcium transport {ECO:0000256|RuleBase:RU365028};
KW Ion transport {ECO:0000256|RuleBase:RU365028};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU365028};
KW Reference proteome {ECO:0000313|Proteomes:UP000016933};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU365028};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU365028}; Transport {ECO:0000256|RuleBase:RU365028};
KW Vacuole {ECO:0000256|RuleBase:RU365028}.
FT TRANSMEM 50..68
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 74..92
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 104..127
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 139..164
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 176..195
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 215..236
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 274..292
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 374..391
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT TRANSMEM 398..418
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU365028"
FT DOMAIN 72..237
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT DOMAIN 273..416
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 459 AA; 50872 MW; 15863E15F940395C CRC64;
MYNEDGHRVN HNGHKVTKGI QPDGESGRSW FHPLYFFRII WSSSCAASKW TNLLWPFTIA
ALVLYFHYPQ HQLWVFICAY IGMVPSANLV GFAGQELARK LPKVAGVMLE TTFGSIVEII
LFTVLIVQGG NDNVTVIRAA ILGSILANLL LCLGLCFFVG GIFHPQQTFH EAISEVGSNL
MLVAGMALVI PTIYYNTLYG RFDTGDGLVE LEREALKISR ATAIVLVVAF AVYVFFQARS
HHGLYEDILQ ADEERDHDRH KDLRKPKLTF TESIVALMLA LMFVSFMAVF LVKEIHFMVD
ERHISDAFVG LILIPVVEKA AEHITAIDEA YDNQMNFALS HVLGASIQTA LLNTPIVVFV
GWGLGYDMSL NFELFDAVVL ILAIMVVGNF LRDEKSDYLE GALCVFVYIL IAVTAWYYPN
PLTEGGTSSA ESSSSPTEGG DPAAIASEAA QRMVRIMMN
//
