ID N2BSH1_9ACTN Unreviewed; 432 AA.
AC N2BSH1;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=Peptidase T {ECO:0000313|EMBL:EMZ41493.1};
GN ORFNames=HMPREF1091_00467 {ECO:0000313|EMBL:EMZ41493.1};
OS Atopobium minutum 10063974.
OC Bacteria; Actinomycetota; Coriobacteriia; Coriobacteriales; Atopobiaceae;
OC Atopobium.
OX NCBI_TaxID=997872 {ECO:0000313|EMBL:EMZ41493.1, ECO:0000313|Proteomes:UP000012651};
RN [1] {ECO:0000313|EMBL:EMZ41493.1, ECO:0000313|Proteomes:UP000012651}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10063974 {ECO:0000313|EMBL:EMZ41493.1,
RC ECO:0000313|Proteomes:UP000012651};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Lambert T., Marvaud J.-C.,
RA Courvalin P., Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M.,
RA Haas B., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M.,
RA Chapman S.B., Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Neiman D.,
RA Pearson M., Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Atopobium minutum 10063974.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR037215-2};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000256|PIRSR:PIRSR037215-
CC 2};
CC -!- SIMILARITY: Belongs to the peptidase M20B family.
CC {ECO:0000256|ARBA:ARBA00009692}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EMZ41493.1}.
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DR EMBL; AGXC01000002; EMZ41493.1; -; Genomic_DNA.
DR RefSeq; WP_002563242.1; NZ_KB822533.1.
DR AlphaFoldDB; N2BSH1; -.
DR PATRIC; fig|997872.3.peg.466; -.
DR HOGENOM; CLU_053676_0_0_11; -.
DR OrthoDB; 9804934at2; -.
DR Proteomes; UP000012651; Unassembled WGS sequence.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0045148; F:tripeptide aminopeptidase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006518; P:peptide metabolic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd03892; M20_peptT; 1.
DR Gene3D; 3.30.70.360; -; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR InterPro; IPR001261; ArgE/DapE_CS.
DR InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR InterPro; IPR002933; Peptidase_M20.
DR InterPro; IPR011650; Peptidase_M20_dimer.
DR InterPro; IPR010161; Peptidase_M20B.
DR NCBIfam; TIGR01882; peptidase-T; 1.
DR PANTHER; PTHR42994; PEPTIDASE T; 1.
DR PANTHER; PTHR42994:SF1; PEPTIDASE T; 1.
DR Pfam; PF07687; M20_dimer; 1.
DR Pfam; PF01546; Peptidase_M20; 1.
DR PIRSF; PIRSF037215; Peptidase_M20B; 1.
DR SUPFAM; SSF55031; Bacterial exopeptidase dimerisation domain; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
DR PROSITE; PS00759; ARGE_DAPE_CPG2_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR037215-2};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000012651};
KW Zinc {ECO:0000256|PIRSR:PIRSR037215-2}.
FT DOMAIN 228..331
FT /note="Peptidase M20 dimerisation"
FT /evidence="ECO:0000259|Pfam:PF07687"
FT ACT_SITE 100
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-1"
FT ACT_SITE 196
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-1"
FT BINDING 98
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
FT BINDING 162
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
FT BINDING 162
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
FT BINDING 197
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
FT BINDING 219
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
FT BINDING 402
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR037215-2"
SQ SEQUENCE 432 AA; 46577 MW; 4D463D3ED6D7C7BC CRC64;
MTDSTTPQNY TGTPIPEGVS DVVARFMRYV QVYTTSDPNN DTQVPSTATQ FDMARMLEAD
LLELGCEDVA VDEHAYVTAT VPASADAQGL PALGLCAHMD SAPDAPGKGV APHIVHYEGG
PLVSGIVNGE PVQSTPEQVP DLDKFVGMDI VCSDGTTLLS ADDKAGVAEI MSLIARLKEH
PEIAHPTLKI AFVPDEEIGH GAELLDLDSF GAAWCYTVDG ETLGEVNYET FSACEANITF
TGVMVHPGSA KDIMVNAITV AREFDALLPA HERPEHTEGY EGFFMPTEIS GDANVVKLQY
IVRDFDSAKF EARQQLLFEI ADFLNKRHGE GTVEISIKPE YRNMAECFEG LEFLVDNALE
ANREAGITDA HCVAVRGGTD GSQLTFRGLP CPNIATGGYN AHSVREFVPV PSLEITVDVL
EHLVTKFAVA QQ
//
