ID N2IJZ6_9PSED Unreviewed; 381 AA.
AC N2IJZ6;
DT 26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT 26-JUN-2013, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE SubName: Full=IS605 OrfB family transposase {ECO:0000313|EMBL:ENA27862.1};
GN ORFNames=HMPREF1487_09045 {ECO:0000313|EMBL:ENA27862.1};
OS Pseudomonas sp. HPB0071.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1203578 {ECO:0000313|EMBL:ENA27862.1, ECO:0000313|Proteomes:UP000017050};
RN [1] {ECO:0000313|EMBL:ENA27862.1, ECO:0000313|Proteomes:UP000017050}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HPB0071 {ECO:0000313|EMBL:ENA27862.1,
RC ECO:0000313|Proteomes:UP000017050};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Schmidt T., Dover J., Dai D.,
RA Walker B., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B.,
RA Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., McCowan C., Murphy C., Neiman D., Pearson M.,
RA Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Pseudomonas sp. HPB0071.";
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transposase 35
CC family. {ECO:0000256|ARBA:ARBA00008761}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the transposase 2
CC family. {ECO:0000256|ARBA:ARBA00011044}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ENA27862.1}.
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DR EMBL; AQFP01000021; ENA27862.1; -; Genomic_DNA.
DR RefSeq; WP_010799198.1; NZ_KI517369.1.
DR AlphaFoldDB; N2IJZ6; -.
DR PATRIC; fig|1203578.3.peg.4790; -.
DR HOGENOM; CLU_032903_0_0_6; -.
DR OrthoDB; 5915636at2; -.
DR Proteomes; UP000017050; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0032196; P:transposition; IEA:UniProtKB-KW.
DR InterPro; IPR010095; Cas12f1-like_TNB.
DR InterPro; IPR001959; Transposase.
DR InterPro; IPR021027; Transposase_put_HTH.
DR NCBIfam; NF040570; guided_TnpB; 1.
DR NCBIfam; TIGR01766; IS200/IS605 family accessory protein TnpB-like domain; 1.
DR PANTHER; PTHR30405:SF20; RNA-GUIDED DNA ENDONUCLEASE INSQ-RELATED; 1.
DR PANTHER; PTHR30405; TRANSPOSASE; 1.
DR Pfam; PF12323; HTH_OrfB_IS605; 1.
DR Pfam; PF01385; OrfB_IS605; 1.
DR Pfam; PF07282; OrfB_Zn_ribbon; 1.
PE 3: Inferred from homology;
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000017050};
KW Transposition {ECO:0000256|ARBA:ARBA00022578};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 1..46
FT /note="Transposase putative helix-turn-helix"
FT /evidence="ECO:0000259|Pfam:PF12323"
FT DOMAIN 171..275
FT /note="Probable transposase IS891/IS1136/IS1341"
FT /evidence="ECO:0000259|Pfam:PF01385"
FT DOMAIN 288..356
FT /note="Cas12f1-like TNB"
FT /evidence="ECO:0000259|Pfam:PF07282"
SQ SEQUENCE 381 AA; 42833 MW; 65216814B1F0F80E CRC64;
MTKRAYRYRF YPTPEQAELL AKTFGCVRLV YNTLLRWRTD AFHKEQQKIG YSAASAYLTA
LKKQPELAFL NEVSCVPLQQ SLRHQQSAFK NFFEGRARYP TFKKKRNHQS AEFTASAFKY
RDGELFLAKS SEPLNIRWSR ALPSAPSTIT VSRDAAGRYF VSCLCEFEPQ VLPVTPKIIG
IDMGLKELFV PSEGEPVANA RHTAKYASRL ALAQRRLSRK KLGSANRAKA RQKVARIHAR
IADCRLDGLH KLSRRLINEN QVVCVENLTV KNMIRNPRLS KAIADAGWGE LVRQLQYKGA
WAGRQVVAID RWFPSSKRCS GCGFVMDLMP LSVRSWVCPQ CKAEHDRDVN AALNIKAAGL
AVLALGANVS GRGQEPVSCS Q
//
