ID   N9UVD2_9SPHN            Unreviewed;       232 AA.
AC   N9UVD2;
DT   26-JUN-2013, integrated into UniProtKB/TrEMBL.
DT   26-JUN-2013, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   RecName: Full=carboxymethylenebutenolidase {ECO:0000256|ARBA:ARBA00044062};
DE            EC=3.1.1.45 {ECO:0000256|ARBA:ARBA00044062};
GN   ORFNames=EBMC1_06064 {ECO:0000313|EMBL:ENY82430.1};
OS   Sphingopyxis sp. MC1.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingopyxis.
OX   NCBI_TaxID=1174684 {ECO:0000313|EMBL:ENY82430.1, ECO:0000313|Proteomes:UP000013072};
RN   [1] {ECO:0000313|EMBL:ENY82430.1, ECO:0000313|Proteomes:UP000013072}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MC1 {ECO:0000313|EMBL:ENY82430.1,
RC   ECO:0000313|Proteomes:UP000013072};
RA   Lolas I.B., Kjeldal H., Almeida B., Le-Quy V., Gough H.L., Nielsen J.L.;
RT   "Differential expression analysis of membrane associated proteins from
RT   triclosan-degrading sphingopyxis.";
RL   Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC         Evidence={ECO:0000256|ARBA:ARBA00043789};
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000256|ARBA:ARBA00008456}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ENY82430.1}.
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DR   EMBL; AOUN01000003; ENY82430.1; -; Genomic_DNA.
DR   RefSeq; WP_003042109.1; NZ_AOUN01000003.1.
DR   AlphaFoldDB; N9UVD2; -.
DR   PATRIC; fig|1174684.3.peg.1223; -.
DR   eggNOG; COG0412; Bacteria.
DR   Proteomes; UP000013072; Unassembled WGS sequence.
DR   GO; GO:0016787; F:hydrolase activity; IEA:InterPro.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   PANTHER; PTHR46623; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR   PANTHER; PTHR46623:SF11; CARBOXYMETHYLENEBUTENOLIDASE-RELATED; 1.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000013072}.
FT   DOMAIN          17..230
FT                   /note="Dienelactone hydrolase"
FT                   /evidence="ECO:0000259|Pfam:PF01738"
SQ   SEQUENCE   232 AA;  25224 MW;  8FB8987DBE225730 CRC64;
     MSPTNSEIAA LDGEGRIPAY VARPDADTTR AIIVIPEIFG VNEGIRKKAD DWAALGYLAI
     APDIFWRFAP GVELSPDIES EFQEALGYFG QYDADLGVKD IEAAIRWLRS QGATKVGCVG
     FCLGGRLAYM AATRTDIDAS VGYYGVMIDT MLNEAHAIAK PLMLHIPTED HFVDHAAQAK
     IHEALDPNPK VTLHDYPGLD HGFAATMGNR RNDDGAQLAD SRTRDFFAEA LA
//