ID NECA1_MOUSE Reviewed; 352 AA.
AC Q8BG18; Q80W91;
DT 03-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 24-JAN-2024, entry version 153.
DE RecName: Full=N-terminal EF-hand calcium-binding protein 1;
DE Short=EF-hand calcium-binding protein 1;
GN Name=Necab1; Synonyms=Efcbp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Kunming;
RA Wu H., Yu L., Li D.;
RT "Cloning and characterization of human and mouse NECAB1.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Head, Liver, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC TISSUE=Brain;
RX PubMed=12044471; DOI=10.1016/s0306-4522(02)00063-5;
RA Sugita S., Ho A., Suedhof T.C.;
RT "NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual
RT domain structure and a restricted expression pattern.";
RL Neuroscience 112:51-63(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-192 AND SER-197, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Interacts with STX1. May interact with CPNE6.
CC {ECO:0000250|UniProtKB:Q8N987}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12044471}.
CC -!- TISSUE SPECIFICITY: Expressed in brain (at protein level). Expressed in
CC the cerebral cortex only in layer 4, thalamic nuclei (the mediodorsal
CC nucleus), hippocampus (a small band of pyramidal neurons at the
CC boundary between CA1 and CA3), interneurons interspersed throughout the
CC hippocampus proper, interneurons in the hilus, bodies of the neurons
CC but also their dendritic projections (at protein level).
CC {ECO:0000269|PubMed:12044471}.
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DR EMBL; AY278200; AAP32077.1; -; mRNA.
DR EMBL; AK039240; BAC30290.1; -; mRNA.
DR EMBL; AK050307; BAC34179.1; -; mRNA.
DR EMBL; AK081951; BAC38379.1; -; mRNA.
DR EMBL; AL732571; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL831792; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL929383; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC067055; AAH67055.1; -; mRNA.
DR CCDS; CCDS17982.1; -.
DR RefSeq; NP_848732.3; NM_178617.4.
DR AlphaFoldDB; Q8BG18; -.
DR BioGRID; 213379; 1.
DR STRING; 10090.ENSMUSP00000038165; -.
DR iPTMnet; Q8BG18; -.
DR MetOSite; Q8BG18; -.
DR PhosphoSitePlus; Q8BG18; -.
DR MaxQB; Q8BG18; -.
DR PaxDb; 10090-ENSMUSP00000038165; -.
DR ProteomicsDB; 252800; -.
DR Antibodypedia; 6629; 252 antibodies from 22 providers.
DR DNASU; 69352; -.
DR Ensembl; ENSMUST00000041606.14; ENSMUSP00000038165.8; ENSMUSG00000040536.16.
DR Ensembl; ENSMUST00000108273.2; ENSMUSP00000103908.2; ENSMUSG00000040536.16.
DR GeneID; 69352; -.
DR KEGG; mmu:69352; -.
DR UCSC; uc008sbi.2; mouse.
DR AGR; MGI:1916602; -.
DR CTD; 64168; -.
DR MGI; MGI:1916602; Necab1.
DR VEuPathDB; HostDB:ENSMUSG00000040536; -.
DR eggNOG; ENOG502QWRY; Eukaryota.
DR GeneTree; ENSGT00950000183131; -.
DR HOGENOM; CLU_041553_0_0_1; -.
DR InParanoid; Q8BG18; -.
DR OMA; EVLLIQW; -.
DR OrthoDB; 2900383at2759; -.
DR PhylomeDB; Q8BG18; -.
DR TreeFam; TF331029; -.
DR BioGRID-ORCS; 69352; 2 hits in 76 CRISPR screens.
DR ChiTaRS; Necab1; mouse.
DR PRO; PR:Q8BG18; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8BG18; Protein.
DR Bgee; ENSMUSG00000040536; Expressed in medial dorsal nucleus of thalamus and 73 other cell types or tissues.
DR Genevisible; Q8BG18; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0001835; P:blastocyst hatching; IMP:MGI.
DR GO; GO:0042984; P:regulation of amyloid precursor protein biosynthetic process; IBA:GO_Central.
DR Gene3D; 3.30.70.100; -; 1.
DR Gene3D; 1.10.238.10; EF-hand; 1.
DR InterPro; IPR007138; ABM_dom.
DR InterPro; IPR011008; Dimeric_a/b-barrel.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR039862; NECAB1/2/3.
DR PANTHER; PTHR12178; EF-HAND DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR12178:SF11; N-TERMINAL EF-HAND CALCIUM-BINDING PROTEIN 1; 1.
DR Pfam; PF03992; ABM; 1.
DR SMART; SM00054; EFh; 2.
DR SUPFAM; SSF54909; Dimeric alpha+beta barrel; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS51725; ABM; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 1: Evidence at protein level;
KW Calcium; Coiled coil; Cytoplasm; Metal-binding; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..352
FT /note="N-terminal EF-hand calcium-binding protein 1"
FT /id="PRO_0000282610"
FT DOMAIN 26..61
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 60..95
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 252..340
FT /note="ABM"
FT REGION 155..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 135..163
FT /evidence="ECO:0000255"
FT COILED 209..275
FT /evidence="ECO:0000255"
FT BINDING 39
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 41
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 43
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 45
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 50
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9ESB5"
FT MOD_RES 192
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 197
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 244
FT /note="E -> G (in Ref. 1; AAP32077)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 352 AA; 40934 MW; D2B069AA7BA97036 CRC64;
MEDSRETSPS SNNSSEELSS TLQLSKGMSI FLDILRRADK NDDGKLSFEE FKAYFADGVL
SGEELHELFH TIDTHNTNNL DTEELCEYFS QHLGEYENVL AALEDLNLSI LKAMGKTKKD
YQEASNLEQF VTRFLLKETL NQLQSLQNSL ECAMETTEEQ TRQERQGPSK PEVLSIQWPG
KRSSRRVQRH NSFSPNSPQF NVSSPALLEE DNQWMTQINR LQKLIDRLEK KDLKLEPLEE
EIIEENTKPH IMLVQRQMSV TEEDLEEFQL ALKHYVESAS AQSGCLRISI QKLSNESRYM
IYEFWENSSV WNRHLQTNYS KTFQRSNVDF LETPELTSTM LVPASWWILN NN
//
