ID NGAP_DICDI Reviewed; 877 AA.
AC Q54Y08;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 110.
DE RecName: Full=Probable Ras GTPase-activating-like protein ngap;
DE Short=DdNGAP;
GN Name=ngap; ORFNames=DDB_G0278483;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP INDUCTION [LARGE SCALE ANALYSIS].
RX PubMed=18590548; DOI=10.1186/1471-2180-8-109;
RA Carilla-Latorre S., Calvo-Garrido J., Bloomfield G., Skelton J., Kay R.R.,
RA Ivens A., Martinez J.L., Escalante R.;
RT "Dictyostelium transcriptional responses to Pseudomonas aeruginosa: common
RT and specific effects from PAO1 and PA14 strains.";
RL BMC Microbiol. 8:109-109(2008).
CC -!- FUNCTION: May function as a Ras GTPase-activating protein.
CC {ECO:0000250}.
CC -!- INDUCTION: Up-regulated by P.aeruginosa, PAO1 strain and PA14 strain
CC infection. {ECO:0000269|PubMed:18590548}.
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DR EMBL; AAFI02000023; EAL68414.1; -; Genomic_DNA.
DR RefSeq; XP_642394.1; XM_637302.1.
DR AlphaFoldDB; Q54Y08; -.
DR SMR; Q54Y08; -.
DR STRING; 44689.Q54Y08; -.
DR PaxDb; 44689-DDB0220496; -.
DR EnsemblProtists; EAL68414; EAL68414; DDB_G0278483.
DR GeneID; 8621599; -.
DR KEGG; ddi:DDB_G0278483; -.
DR dictyBase; DDB_G0278483; ngap.
DR eggNOG; KOG1032; Eukaryota.
DR eggNOG; KOG2059; Eukaryota.
DR HOGENOM; CLU_328024_0_0_1; -.
DR InParanoid; Q54Y08; -.
DR OMA; LMPDHPG; -.
DR Reactome; R-DDI-5218921; VEGFR2 mediated cell proliferation.
DR Reactome; R-DDI-5658442; Regulation of RAS by GAPs.
DR Reactome; R-DDI-8849471; PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases.
DR PRO; PR:Q54Y08; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005813; C:centrosome; TAS:dictyBase.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0031256; C:leading edge membrane; IDA:dictyBase.
DR GO; GO:0005096; F:GTPase activator activity; IMP:dictyBase.
DR GO; GO:0005543; F:phospholipid binding; IDA:dictyBase.
DR GO; GO:0046580; P:negative regulation of Ras protein signal transduction; IMP:dictyBase.
DR GO; GO:0051592; P:response to calcium ion; IMP:dictyBase.
DR CDD; cd00030; C2; 1.
DR CDD; cd05128; RasGAP_GAP1_like; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR039360; Ras_GTPase.
DR InterPro; IPR023152; RasGAP_CS.
DR InterPro; IPR001936; RasGAP_dom.
DR InterPro; IPR008936; Rho_GTPase_activation_prot.
DR PANTHER; PTHR10194; RAS GTPASE-ACTIVATING PROTEINS; 1.
DR PANTHER; PTHR10194:SF149; RAS GTPASE-ACTIVATING-LIKE PROTEIN NGAP-RELATED; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00616; RasGAP; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00323; RasGAP; 1.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF48350; GTPase activation domain, GAP; 1.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS00509; RAS_GTPASE_ACTIV_1; 1.
DR PROSITE; PS50018; RAS_GTPASE_ACTIV_2; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; GTPase activation; Reference proteome.
FT CHAIN 1..877
FT /note="Probable Ras GTPase-activating-like protein ngap"
FT /id="PRO_0000393333"
FT DOMAIN 72..218
FT /note="C2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00041"
FT DOMAIN 575..769
FT /note="Ras-GAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00167"
FT REGION 350..456
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 820..848
FT /evidence="ECO:0000255"
FT COMPBIAS 359..456
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 877 AA; 98378 MW; 67BF5A9BC3CD49E5 CRC64;
MMESLTVNEN TINEFEIKND IIIEQQQIQS KTSIPLLNIK DGNTTTTNTI NEINNNNNNI
ETSTMSRKSL MTPSATYESL IRQQKTKDCN LTVKVFEARN LIEARLRKRE MVKNGKSFKR
AQNLLTEISS PNLMTFSDTT DPYCTVQLEK QKQRTRTIPK KLNPFWCEEF QLEISDPASA
KLVLSVMDEK KYSNDEHIGK LVIPINTLKD QKERELWFPL TQPQSSKKVP QIQILFNFKP
ISLTDPSQPG HIQWKVLFGR NLSHSLATPL PSMSAASMGG STSTENISNV YTNTTPFINW
SVRSKKGDIV IDEEGIAWQD ALTNGITREL KDSIECITFT LWRYEPKTFS DDGDISGLKS
PRGTNSSASG SGFIPSTPPP SSHQKSNDTT ATTTPSSTPS TPISPSSQSN NIKTPDSKTR
SSSNASTNTP QTTPKSTGTS GGPAPPETIN LSSSINSTSS LISNGSINVI DSSNNLHHHH
NNGTNIMNPL KDGYEQYFIG QGIVLASHID IEKPNDQWLC LYPKQTLDNK FGDIRLKLKY
SEEVVLPLQS YQPLLELLQQ ENLYTITLLG KVTKHRESVS NNLIRVFEKT GKCLYLLKSL
TDHEIDSTNN PDIIFRGNSL ATKSVDLFMK LIGIPYLSQT IGPLIKKIYS SKKSCEIDPT
KLEKGEDIKK NCKNLLSWVK KMTTAILSSV NNCPGPLREV FKSIQDKVVQ RYPKDEITRY
TAVSGFIFLR FFCPAILAPK LFDLMPDHPG IKTTRSLILI AKTLQNLANQ VEFGEYKEDF
MKDMNRFVID NMENMKSFIN TLSTVPADCP PGALQSPIIL EKELACLYRH LIKQRQDMAE
EMESTESEPK TQQEKDSFGK LIKILNSLDE DVQLASN
//
