ID NIFE_BRADU Reviewed; 547 AA.
AC P26506; Q9ANN3;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2003, sequence version 2.
DT 27-MAR-2024, entry version 121.
DE RecName: Full=Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE;
GN Name=nifE; OrderedLocusNames=blr1745;
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=224911;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=2266945; DOI=10.1007/bf00262436;
RA Aguilar O.M., Taormino J., Thoeny B., Ramseier T., Hennecke H.,
RA Szalay A.A.;
RT "The nifEN genes participating in FeMo cofactor biosynthesis and genes
RT encoding dinitrogenase are part of the same operon in Bradyrhizobium
RT species.";
RL Mol. Gen. Genet. 224:413-420(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=USDA 110spc4;
RX PubMed=11157954; DOI=10.1128/jb.183.4.1405-1412.2001;
RA Goettfert M., Roethlisberger S., Kuendig C., Beck C., Marty R.,
RA Hennecke H.;
RT "Potential symbiosis-specific genes uncovered by sequencing a 410-kb DNA
RT region of the Bradyrhizobium japonicum chromosome.";
RL J. Bacteriol. 183:1405-1412(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
CC -!- FUNCTION: This protein may play a role in the biosynthesis of the
CC prosthetic group of nitrogenase (FeMo cofactor).
CC -!- PATHWAY: Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.
CC -!- SIMILARITY: Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}.
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DR EMBL; X56894; CAA40213.1; -; Genomic_DNA.
DR EMBL; AH010242; AAG60731.1; -; Genomic_DNA.
DR EMBL; BA000040; BAC47010.1; -; Genomic_DNA.
DR PIR; S12263; NIZJME.
DR RefSeq; NP_768385.1; NC_004463.1.
DR RefSeq; WP_011084554.1; NZ_CP011360.1.
DR AlphaFoldDB; P26506; -.
DR SMR; P26506; -.
DR STRING; 224911.AAV28_05655; -.
DR EnsemblBacteria; BAC47010; BAC47010; BAC47010.
DR GeneID; 83314015; -.
DR KEGG; bja:blr1745; -.
DR PATRIC; fig|224911.44.peg.1210; -.
DR eggNOG; COG2710; Bacteria.
DR HOGENOM; CLU_025876_1_1_5; -.
DR InParanoid; P26506; -.
DR OrthoDB; 9762718at2; -.
DR PhylomeDB; P26506; -.
DR UniPathway; UPA00782; -.
DR Proteomes; UP000002526; Chromosome.
DR GO; GO:0016163; F:nitrogenase activity; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR GO; GO:0065003; P:protein-containing complex assembly; IEA:InterPro.
DR CDD; cd01968; Nitrogenase_NifE_I; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.50.12380; Nitrogenase MoFe cofactor biosynthesis protein NifE, C-terminal; 1.
DR Gene3D; 3.40.50.1980; Nitrogenase molybdenum iron protein domain; 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR000510; Nase/OxRdtase_comp1.
DR InterPro; IPR000318; Nase_comp1_CS.
DR InterPro; IPR005973; NifE.
DR NCBIfam; TIGR01283; nifE; 1.
DR PANTHER; PTHR42956; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR PANTHER; PTHR42956:SF1; NITROGENASE IRON-MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN NIFE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF00148; Oxidored_nitro; 1.
DR SUPFAM; SSF53807; Helical backbone' metal receptor; 1.
DR PROSITE; PS00699; NITROGENASE_1_1; 1.
DR PROSITE; PS00090; NITROGENASE_1_2; 1.
PE 3: Inferred from homology;
KW Nitrogen fixation; Reference proteome.
FT CHAIN 1..547
FT /note="Nitrogenase iron-molybdenum cofactor biosynthesis
FT protein NifE"
FT /id="PRO_0000153113"
FT CONFLICT 58
FT /note="T -> N (in Ref. 1; CAA40213)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="G -> A (in Ref. 1; CAA40213)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 547 AA; 59509 MW; F91DDCCF3984308B CRC64;
MSSLAATVQD IFDEPGCAKN GSKSEAERRN GCTRQLQPGS AAGGCAFDGA KVALQPFTDV
AHLVHGPIAC EGNSWDNRGA ASSGSDLWRT AFTTDLSETD IVFGGEKRLC KAIKEIIDKC
DPPAIFVYQT CIPAMIGDDI NAVCKAASRR FSKPVIPINS PGFAGSKNLG NKLAGEALLD
YVIGTREPDY TTPYDINLIG EYNLSGELWQ VKPVLDELGV RILCCISGDG KYREVASSHR
ARAAMLVCSK SMINVARKME QRYGIPFFEG SFYGIQDSSE SLRQIARLLV ERGAPADLLG
RTEAVIAREE ARAWAAIQPY KPRLEGKRAL LMTGGVKSWS VVSALQEAGL ELVGTSVKKS
TMEDKERIKE LMGQDAHMID DMTAREMYKM LKDAEADIML SGGKSQFVAL KAAVPWVDIN
QERCHAYMGY AGIVKLVEEI DNSLSSPMWE QLRRPAPWEA LAKAREQMQS MAAIAGDPVL
AETARRARNI CVCNRVDLGT IEDAISVHGL RSVAAVREHT NAAGGCCQGR IEDMLMSEPS
DMRQAAE
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